Monitoring the banana response to infection by Mycosphaerella fijiensis : N76
Mycosphaerella fijiensis is a hemibiotrophic ascomycete causing the black leaf streak disease (BLSD) of banana that is commonly called Black Sigatoka disease. BLSD is regarded as the most economically important threat to banana production and affects almost all edible banana cultivars grown worldwide. The M. fijiensis infection cycle goes through a long symptomless phase in the plant, during which the fungus grows in the mesophyll intercellular space, before the appearance of disease symptoms in the form of necrotic areas. Despite the economical importance of the BLSD disease, little is known about the physiological and molecular events occurring during the pathogen life cycle in the plant and on the genetic bases of resistance. To better characterize the interaction, we first established a bioassay based on detached leaves maintained in vitro, associated to phenotyping with an image analysis software package (Visilog® Noesis). This allows higher throughput phenotyping and more precise monitoring of the reaction of banana accessions to M. fijiensis strains. In a preliminary experiment, we also analysed transcriptome changes in three accessions showing contrasted reactions to M. fijiensis, using RNA-Seq. First results of this analysis will be presented. (Texte intégral)
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dig-cirad-fr-5686482021-01-04T12:20:05Z http://agritrop.cirad.fr/568648/ http://agritrop.cirad.fr/568648/ Monitoring the banana response to infection by Mycosphaerella fijiensis : N76. Carreel Françoise, Da Silva Corinne, Labadie Karine, Souquet Marlène, Habas Rémy, Pignolet Luc, Zapater Marie-Françoise, Yahiaoui Nabila. 2013. In : International conference "Plant Diseases and Resistance Mechanisms" programme and abstracts, Vienna, Austria, February 20-22th 2013. Vienna International Plant Conference Association (VIPCA). Vienna : VIPCA, Résumé, 73. International Conference "Plant Diseases and Resistance Mechanisms", Vienne, Autriche, 20 Février 2013/22 Février 2013. Researchers Monitoring the banana response to infection by Mycosphaerella fijiensis : N76 Carreel, Françoise Da Silva, Corinne Labadie, Karine Souquet, Marlène Habas, Rémy Pignolet, Luc Zapater, Marie-Françoise Yahiaoui, Nabila eng 2013 VIPCA International conference "Plant Diseases and Resistance Mechanisms" programme and abstracts, Vienna, Austria, February 20-22th 2013 H20 - Maladies des plantes U30 - Méthodes de recherche Mycosphaerella fijiensis is a hemibiotrophic ascomycete causing the black leaf streak disease (BLSD) of banana that is commonly called Black Sigatoka disease. BLSD is regarded as the most economically important threat to banana production and affects almost all edible banana cultivars grown worldwide. The M. fijiensis infection cycle goes through a long symptomless phase in the plant, during which the fungus grows in the mesophyll intercellular space, before the appearance of disease symptoms in the form of necrotic areas. Despite the economical importance of the BLSD disease, little is known about the physiological and molecular events occurring during the pathogen life cycle in the plant and on the genetic bases of resistance. To better characterize the interaction, we first established a bioassay based on detached leaves maintained in vitro, associated to phenotyping with an image analysis software package (Visilog® Noesis). This allows higher throughput phenotyping and more precise monitoring of the reaction of banana accessions to M. fijiensis strains. In a preliminary experiment, we also analysed transcriptome changes in three accessions showing contrasted reactions to M. fijiensis, using RNA-Seq. First results of this analysis will be presented. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/568648/1/document_568648.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html |
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H20 - Maladies des plantes U30 - Méthodes de recherche H20 - Maladies des plantes U30 - Méthodes de recherche Carreel, Françoise Da Silva, Corinne Labadie, Karine Souquet, Marlène Habas, Rémy Pignolet, Luc Zapater, Marie-Françoise Yahiaoui, Nabila Monitoring the banana response to infection by Mycosphaerella fijiensis : N76 |
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Mycosphaerella fijiensis is a hemibiotrophic ascomycete causing the black leaf streak disease (BLSD) of banana that is commonly called Black Sigatoka disease. BLSD is regarded as the most economically important threat to banana production and affects almost all edible banana cultivars grown worldwide. The M. fijiensis infection cycle goes through a long symptomless phase in the plant, during which the fungus grows in the mesophyll intercellular space, before the appearance of disease symptoms in the form of necrotic areas. Despite the economical importance of the BLSD disease, little is known about the physiological and molecular events occurring during the pathogen life cycle in the plant and on the genetic bases of resistance. To better characterize the interaction, we first established a bioassay based on detached leaves maintained in vitro, associated to phenotyping with an image analysis software package (Visilog® Noesis). This allows higher throughput phenotyping and more precise monitoring of the reaction of banana accessions to M. fijiensis strains. In a preliminary experiment, we also analysed transcriptome changes in three accessions showing contrasted reactions to M. fijiensis, using RNA-Seq. First results of this analysis will be presented. (Texte intégral) |
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conference_item |
topic_facet |
H20 - Maladies des plantes U30 - Méthodes de recherche |
author |
Carreel, Françoise Da Silva, Corinne Labadie, Karine Souquet, Marlène Habas, Rémy Pignolet, Luc Zapater, Marie-Françoise Yahiaoui, Nabila |
author_facet |
Carreel, Françoise Da Silva, Corinne Labadie, Karine Souquet, Marlène Habas, Rémy Pignolet, Luc Zapater, Marie-Françoise Yahiaoui, Nabila |
author_sort |
Carreel, Françoise |
title |
Monitoring the banana response to infection by Mycosphaerella fijiensis : N76 |
title_short |
Monitoring the banana response to infection by Mycosphaerella fijiensis : N76 |
title_full |
Monitoring the banana response to infection by Mycosphaerella fijiensis : N76 |
title_fullStr |
Monitoring the banana response to infection by Mycosphaerella fijiensis : N76 |
title_full_unstemmed |
Monitoring the banana response to infection by Mycosphaerella fijiensis : N76 |
title_sort |
monitoring the banana response to infection by mycosphaerella fijiensis : n76 |
publisher |
VIPCA |
url |
http://agritrop.cirad.fr/568648/ http://agritrop.cirad.fr/568648/1/document_568648.pdf |
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