Xanthomonas albilineans needs an OmpA family outer protein for disease symptom development and multiplication in the sugarcane stalk : [Abstracts]
Xanthomonas albilineans (Xa) is a systemic, xylem-invading pathogen that causes sugarcane leaf scald. Xa produces albicidin, a potent antibiotic and phytotoxin which blocks chloroplast differentiation, thus causing the foliar symptoms of the disease. Albicidin is the only known pathogenicity factor in Xa, yet albicidin deficient mutants are still able to colonize the sugarcane plant. In an attempt to identify other major pathogenicity factors, we screened 1,216 independent Tn5 insertions in Xa strain XaFL07-1 by single inoculation onto sugarcane cultivar CP80-1743. Mutants were screened for reduced pathogenicity (i.e., capacity to induce leaf symptoms and to multiply in the sugarcane stalk). Five independent Tn5 insertions were found in gene XALc_0557, which is predicted to encode an OmpA family outer membrane protein. Each of these insertions resulted in a mutant strain that elicited very slight to no symptoms and was not able to move as efficiently within the sugarcane stalk, both spatially and in intensity, as wild type XaFL07-1. Additional phenotypic studies showed that these mutants: 1) produced albicidin, 2) were less motile and 3) were slower growing than the wild type Xa in vitro. However, these OmpA mutants were able to multiply in sugarcane leaf tissue to levels similar to the wild-type strain XaFL07-1. Complementation analyses are currently underway. (Texte intégral)
Main Authors: | , , , , |
---|---|
Format: | article biblioteca |
Language: | eng |
Subjects: | H20 - Maladies des plantes, Xanthomonas albilineans, Saccharum officinarum, mutant, testage, pouvoir pathogène, protéine microbienne, http://aims.fao.org/aos/agrovoc/c_27422, http://aims.fao.org/aos/agrovoc/c_6727, http://aims.fao.org/aos/agrovoc/c_5013, http://aims.fao.org/aos/agrovoc/c_7683, http://aims.fao.org/aos/agrovoc/c_5629, http://aims.fao.org/aos/agrovoc/c_12537, |
Online Access: | http://agritrop.cirad.fr/568040/ http://agritrop.cirad.fr/568040/1/document_568040.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-568040 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5680402024-01-28T21:15:51Z http://agritrop.cirad.fr/568040/ http://agritrop.cirad.fr/568040/ Xanthomonas albilineans needs an OmpA family outer protein for disease symptom development and multiplication in the sugarcane stalk : [Abstracts]. Rott Philippe, Fleites Laura, Marlow Gary C., Royer Monique, Gabriel Dean W.. 2010. Phytopathology, 100 (6S), suppl., Résumé, S111 2010 APS Annual Meeting, Charlotte, États-Unis, 7 Août 2010/11 Août 2010.http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2010.100.6.S1 <http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2010.100.6.S1> Xanthomonas albilineans needs an OmpA family outer protein for disease symptom development and multiplication in the sugarcane stalk : [Abstracts] Rott, Philippe Fleites, Laura Marlow, Gary C. Royer, Monique Gabriel, Dean W. eng 2010 Phytopathology H20 - Maladies des plantes Xanthomonas albilineans Saccharum officinarum mutant testage pouvoir pathogène protéine microbienne http://aims.fao.org/aos/agrovoc/c_27422 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_5013 http://aims.fao.org/aos/agrovoc/c_7683 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_12537 Xanthomonas albilineans (Xa) is a systemic, xylem-invading pathogen that causes sugarcane leaf scald. Xa produces albicidin, a potent antibiotic and phytotoxin which blocks chloroplast differentiation, thus causing the foliar symptoms of the disease. Albicidin is the only known pathogenicity factor in Xa, yet albicidin deficient mutants are still able to colonize the sugarcane plant. In an attempt to identify other major pathogenicity factors, we screened 1,216 independent Tn5 insertions in Xa strain XaFL07-1 by single inoculation onto sugarcane cultivar CP80-1743. Mutants were screened for reduced pathogenicity (i.e., capacity to induce leaf symptoms and to multiply in the sugarcane stalk). Five independent Tn5 insertions were found in gene XALc_0557, which is predicted to encode an OmpA family outer membrane protein. Each of these insertions resulted in a mutant strain that elicited very slight to no symptoms and was not able to move as efficiently within the sugarcane stalk, both spatially and in intensity, as wild type XaFL07-1. Additional phenotypic studies showed that these mutants: 1) produced albicidin, 2) were less motile and 3) were slower growing than the wild type Xa in vitro. However, these OmpA mutants were able to multiply in sugarcane leaf tissue to levels similar to the wild-type strain XaFL07-1. Complementation analyses are currently underway. (Texte intégral) article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/568040/1/document_568040.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2010.100.6.S1 info:eu-repo/semantics/altIdentifier/purl/http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2010.100.6.S1 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
H20 - Maladies des plantes Xanthomonas albilineans Saccharum officinarum mutant testage pouvoir pathogène protéine microbienne http://aims.fao.org/aos/agrovoc/c_27422 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_5013 http://aims.fao.org/aos/agrovoc/c_7683 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_12537 H20 - Maladies des plantes Xanthomonas albilineans Saccharum officinarum mutant testage pouvoir pathogène protéine microbienne http://aims.fao.org/aos/agrovoc/c_27422 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_5013 http://aims.fao.org/aos/agrovoc/c_7683 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_12537 |
spellingShingle |
H20 - Maladies des plantes Xanthomonas albilineans Saccharum officinarum mutant testage pouvoir pathogène protéine microbienne http://aims.fao.org/aos/agrovoc/c_27422 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_5013 http://aims.fao.org/aos/agrovoc/c_7683 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_12537 H20 - Maladies des plantes Xanthomonas albilineans Saccharum officinarum mutant testage pouvoir pathogène protéine microbienne http://aims.fao.org/aos/agrovoc/c_27422 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_5013 http://aims.fao.org/aos/agrovoc/c_7683 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_12537 Rott, Philippe Fleites, Laura Marlow, Gary C. Royer, Monique Gabriel, Dean W. Xanthomonas albilineans needs an OmpA family outer protein for disease symptom development and multiplication in the sugarcane stalk : [Abstracts] |
description |
Xanthomonas albilineans (Xa) is a systemic, xylem-invading pathogen that causes sugarcane leaf scald. Xa produces albicidin, a potent antibiotic and phytotoxin which blocks chloroplast differentiation, thus causing the foliar symptoms of the disease. Albicidin is the only known pathogenicity factor in Xa, yet albicidin deficient mutants are still able to colonize the sugarcane plant. In an attempt to identify other major pathogenicity factors, we screened 1,216 independent Tn5 insertions in Xa strain XaFL07-1 by single inoculation onto sugarcane cultivar CP80-1743. Mutants were screened for reduced pathogenicity (i.e., capacity to induce leaf symptoms and to multiply in the sugarcane stalk). Five independent Tn5 insertions were found in gene XALc_0557, which is predicted to encode an OmpA family outer membrane protein. Each of these insertions resulted in a mutant strain that elicited very slight to no symptoms and was not able to move as efficiently within the sugarcane stalk, both spatially and in intensity, as wild type XaFL07-1. Additional phenotypic studies showed that these mutants: 1) produced albicidin, 2) were less motile and 3) were slower growing than the wild type Xa in vitro. However, these OmpA mutants were able to multiply in sugarcane leaf tissue to levels similar to the wild-type strain XaFL07-1. Complementation analyses are currently underway. (Texte intégral) |
format |
article |
topic_facet |
H20 - Maladies des plantes Xanthomonas albilineans Saccharum officinarum mutant testage pouvoir pathogène protéine microbienne http://aims.fao.org/aos/agrovoc/c_27422 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_5013 http://aims.fao.org/aos/agrovoc/c_7683 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_12537 |
author |
Rott, Philippe Fleites, Laura Marlow, Gary C. Royer, Monique Gabriel, Dean W. |
author_facet |
Rott, Philippe Fleites, Laura Marlow, Gary C. Royer, Monique Gabriel, Dean W. |
author_sort |
Rott, Philippe |
title |
Xanthomonas albilineans needs an OmpA family outer protein for disease symptom development and multiplication in the sugarcane stalk : [Abstracts] |
title_short |
Xanthomonas albilineans needs an OmpA family outer protein for disease symptom development and multiplication in the sugarcane stalk : [Abstracts] |
title_full |
Xanthomonas albilineans needs an OmpA family outer protein for disease symptom development and multiplication in the sugarcane stalk : [Abstracts] |
title_fullStr |
Xanthomonas albilineans needs an OmpA family outer protein for disease symptom development and multiplication in the sugarcane stalk : [Abstracts] |
title_full_unstemmed |
Xanthomonas albilineans needs an OmpA family outer protein for disease symptom development and multiplication in the sugarcane stalk : [Abstracts] |
title_sort |
xanthomonas albilineans needs an ompa family outer protein for disease symptom development and multiplication in the sugarcane stalk : [abstracts] |
url |
http://agritrop.cirad.fr/568040/ http://agritrop.cirad.fr/568040/1/document_568040.pdf |
work_keys_str_mv |
AT rottphilippe xanthomonasalbilineansneedsanompafamilyouterproteinfordiseasesymptomdevelopmentandmultiplicationinthesugarcanestalkabstracts AT fleiteslaura xanthomonasalbilineansneedsanompafamilyouterproteinfordiseasesymptomdevelopmentandmultiplicationinthesugarcanestalkabstracts AT marlowgaryc xanthomonasalbilineansneedsanompafamilyouterproteinfordiseasesymptomdevelopmentandmultiplicationinthesugarcanestalkabstracts AT royermonique xanthomonasalbilineansneedsanompafamilyouterproteinfordiseasesymptomdevelopmentandmultiplicationinthesugarcanestalkabstracts AT gabrieldeanw xanthomonasalbilineansneedsanompafamilyouterproteinfordiseasesymptomdevelopmentandmultiplicationinthesugarcanestalkabstracts |
_version_ |
1792498419573456896 |