Reversible oxygen-tolerant hydrogenase carried by free-living N2-fixing bacteria isolated from the rhizospheres of rice, maize, and wheat
Hydrogen production by microorganisms is often described as a promising sustainable and clean energy source, but still faces several obstacles, which prevent practical application. Among them, oxygen sensitivity of hydrogenases represents one of the major limitations hampering the biotechnological implementation of photobiological production processes. Here, we describe a hierarchical biodiversity-based approach, including a chemochromic screening of hydrogenase activity of hundreds of bacterial strains collected from several ecosystems, followed by mass spectrometry measurements of hydrogenase activity of a selection of the H2-oxidizing bacterial strains identified during the screen. In all, 131 of 1266 strains, isolated from cereal rhizospheres and basins containing irradiating waste, were scored as H2-oxidizing bacteria, including Pseudomonas sp., Serratia sp., Stenotrophomonas sp., Enterobacter sp., Rahnella sp., Burkholderia sp., and Ralstonia sp. isolates. Four free-living N2-fixing bacteria harbored a high and oxygen-tolerant hydrogenase activity, which was not fully inhibited within entire cells up to 150-250 lmol/L O2 concentration or within soluble protein extracts up to 25-30 lmol/L. The only hydrogenase-related genes that we could reveal in these strains were of the hyc type (subunits of formate hydrogenlyase complex). The four free-living N2-fixing bacteria were closely related to Enterobacter radicincitans based on the sequences of four genes (16S rRNA, rpoB, hsp60, and hycE genes). These results should bring interesting prospects for microbial biohydrogen production and might have ecophysiological significance for bacterial adaptation to the oxic-anoxic interfaces in the rhizosphere.
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dig-cirad-fr-5671032024-01-28T21:05:34Z http://agritrop.cirad.fr/567103/ http://agritrop.cirad.fr/567103/ Reversible oxygen-tolerant hydrogenase carried by free-living N2-fixing bacteria isolated from the rhizospheres of rice, maize, and wheat. Roumagnac Philippe, Richaud Pierre, Barakat Mohamed, Ortet Philippe, Roncato Marie-Anne, Heulin Thierry, Peltier Gilles, Achouak Wafa, Cournac Laurent. 2012. MicrobiologyOpen, 1 (4) : 349-361.https://doi.org/10.1002/mbo3.37 <https://doi.org/10.1002/mbo3.37> Reversible oxygen-tolerant hydrogenase carried by free-living N2-fixing bacteria isolated from the rhizospheres of rice, maize, and wheat Roumagnac, Philippe Richaud, Pierre Barakat, Mohamed Ortet, Philippe Roncato, Marie-Anne Heulin, Thierry Peltier, Gilles Achouak, Wafa Cournac, Laurent eng 2012 MicrobiologyOpen P34 - Biologie du sol Oryza sativa Zea mays Triticum métabolisme activité enzymatique rhizosphère bactérie fixatrice de l'azote gène hydrogène oxygène Enterobacter Pseudomonas spectrométrie de masse Serratia Burkholderiaceae Stenotrophomonas http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_7950 http://aims.fao.org/aos/agrovoc/c_4769 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_6569 http://aims.fao.org/aos/agrovoc/c_27939 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_3724 http://aims.fao.org/aos/agrovoc/c_5477 http://aims.fao.org/aos/agrovoc/c_23918 http://aims.fao.org/aos/agrovoc/c_6304 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_14210 http://aims.fao.org/aos/agrovoc/c_37846 http://aims.fao.org/aos/agrovoc/c_3efe6f9c France Viet Nam Égypte Sénégal Martinique http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_8227 http://aims.fao.org/aos/agrovoc/c_2503 http://aims.fao.org/aos/agrovoc/c_6970 http://aims.fao.org/aos/agrovoc/c_4635 Hydrogen production by microorganisms is often described as a promising sustainable and clean energy source, but still faces several obstacles, which prevent practical application. Among them, oxygen sensitivity of hydrogenases represents one of the major limitations hampering the biotechnological implementation of photobiological production processes. Here, we describe a hierarchical biodiversity-based approach, including a chemochromic screening of hydrogenase activity of hundreds of bacterial strains collected from several ecosystems, followed by mass spectrometry measurements of hydrogenase activity of a selection of the H2-oxidizing bacterial strains identified during the screen. In all, 131 of 1266 strains, isolated from cereal rhizospheres and basins containing irradiating waste, were scored as H2-oxidizing bacteria, including Pseudomonas sp., Serratia sp., Stenotrophomonas sp., Enterobacter sp., Rahnella sp., Burkholderia sp., and Ralstonia sp. isolates. Four free-living N2-fixing bacteria harbored a high and oxygen-tolerant hydrogenase activity, which was not fully inhibited within entire cells up to 150-250 lmol/L O2 concentration or within soluble protein extracts up to 25-30 lmol/L. The only hydrogenase-related genes that we could reveal in these strains were of the hyc type (subunits of formate hydrogenlyase complex). The four free-living N2-fixing bacteria were closely related to Enterobacter radicincitans based on the sequences of four genes (16S rRNA, rpoB, hsp60, and hycE genes). These results should bring interesting prospects for microbial biohydrogen production and might have ecophysiological significance for bacterial adaptation to the oxic-anoxic interfaces in the rhizosphere. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/567103/1/document_567103.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1002/mbo3.37 10.1002/mbo3.37 info:eu-repo/semantics/altIdentifier/doi/10.1002/mbo3.37 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1002/mbo3.37 |
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P34 - Biologie du sol Oryza sativa Zea mays Triticum métabolisme activité enzymatique rhizosphère bactérie fixatrice de l'azote gène hydrogène oxygène Enterobacter Pseudomonas spectrométrie de masse Serratia Burkholderiaceae Stenotrophomonas http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_7950 http://aims.fao.org/aos/agrovoc/c_4769 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_6569 http://aims.fao.org/aos/agrovoc/c_27939 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_3724 http://aims.fao.org/aos/agrovoc/c_5477 http://aims.fao.org/aos/agrovoc/c_23918 http://aims.fao.org/aos/agrovoc/c_6304 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_14210 http://aims.fao.org/aos/agrovoc/c_37846 http://aims.fao.org/aos/agrovoc/c_3efe6f9c http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_8227 http://aims.fao.org/aos/agrovoc/c_2503 http://aims.fao.org/aos/agrovoc/c_6970 http://aims.fao.org/aos/agrovoc/c_4635 P34 - Biologie du sol Oryza sativa Zea mays Triticum métabolisme activité enzymatique rhizosphère bactérie fixatrice de l'azote gène hydrogène oxygène Enterobacter Pseudomonas spectrométrie de masse Serratia Burkholderiaceae Stenotrophomonas http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_7950 http://aims.fao.org/aos/agrovoc/c_4769 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_6569 http://aims.fao.org/aos/agrovoc/c_27939 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_3724 http://aims.fao.org/aos/agrovoc/c_5477 http://aims.fao.org/aos/agrovoc/c_23918 http://aims.fao.org/aos/agrovoc/c_6304 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_14210 http://aims.fao.org/aos/agrovoc/c_37846 http://aims.fao.org/aos/agrovoc/c_3efe6f9c http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_8227 http://aims.fao.org/aos/agrovoc/c_2503 http://aims.fao.org/aos/agrovoc/c_6970 http://aims.fao.org/aos/agrovoc/c_4635 |
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P34 - Biologie du sol Oryza sativa Zea mays Triticum métabolisme activité enzymatique rhizosphère bactérie fixatrice de l'azote gène hydrogène oxygène Enterobacter Pseudomonas spectrométrie de masse Serratia Burkholderiaceae Stenotrophomonas http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_7950 http://aims.fao.org/aos/agrovoc/c_4769 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_6569 http://aims.fao.org/aos/agrovoc/c_27939 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_3724 http://aims.fao.org/aos/agrovoc/c_5477 http://aims.fao.org/aos/agrovoc/c_23918 http://aims.fao.org/aos/agrovoc/c_6304 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_14210 http://aims.fao.org/aos/agrovoc/c_37846 http://aims.fao.org/aos/agrovoc/c_3efe6f9c http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_8227 http://aims.fao.org/aos/agrovoc/c_2503 http://aims.fao.org/aos/agrovoc/c_6970 http://aims.fao.org/aos/agrovoc/c_4635 P34 - Biologie du sol Oryza sativa Zea mays Triticum métabolisme activité enzymatique rhizosphère bactérie fixatrice de l'azote gène hydrogène oxygène Enterobacter Pseudomonas spectrométrie de masse Serratia Burkholderiaceae Stenotrophomonas http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_7950 http://aims.fao.org/aos/agrovoc/c_4769 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_6569 http://aims.fao.org/aos/agrovoc/c_27939 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_3724 http://aims.fao.org/aos/agrovoc/c_5477 http://aims.fao.org/aos/agrovoc/c_23918 http://aims.fao.org/aos/agrovoc/c_6304 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_14210 http://aims.fao.org/aos/agrovoc/c_37846 http://aims.fao.org/aos/agrovoc/c_3efe6f9c http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_8227 http://aims.fao.org/aos/agrovoc/c_2503 http://aims.fao.org/aos/agrovoc/c_6970 http://aims.fao.org/aos/agrovoc/c_4635 Roumagnac, Philippe Richaud, Pierre Barakat, Mohamed Ortet, Philippe Roncato, Marie-Anne Heulin, Thierry Peltier, Gilles Achouak, Wafa Cournac, Laurent Reversible oxygen-tolerant hydrogenase carried by free-living N2-fixing bacteria isolated from the rhizospheres of rice, maize, and wheat |
description |
Hydrogen production by microorganisms is often described as a promising sustainable and clean energy source, but still faces several obstacles, which prevent practical application. Among them, oxygen sensitivity of hydrogenases represents one of the major limitations hampering the biotechnological implementation of photobiological production processes. Here, we describe a hierarchical biodiversity-based approach, including a chemochromic screening of hydrogenase activity of hundreds of bacterial strains collected from several ecosystems, followed by mass spectrometry measurements of hydrogenase activity of a selection of the H2-oxidizing bacterial strains identified during the screen. In all, 131 of 1266 strains, isolated from cereal rhizospheres and basins containing irradiating waste, were scored as H2-oxidizing bacteria, including Pseudomonas sp., Serratia sp., Stenotrophomonas sp., Enterobacter sp., Rahnella sp., Burkholderia sp., and Ralstonia sp. isolates. Four free-living N2-fixing bacteria harbored a high and oxygen-tolerant hydrogenase activity, which was not fully inhibited within entire cells up to 150-250 lmol/L O2 concentration or within soluble protein extracts up to 25-30 lmol/L. The only hydrogenase-related genes that we could reveal in these strains were of the hyc type (subunits of formate hydrogenlyase complex). The four free-living N2-fixing bacteria were closely related to Enterobacter radicincitans based on the sequences of four genes (16S rRNA, rpoB, hsp60, and hycE genes). These results should bring interesting prospects for microbial biohydrogen production and might have ecophysiological significance for bacterial adaptation to the oxic-anoxic interfaces in the rhizosphere. |
format |
article |
topic_facet |
P34 - Biologie du sol Oryza sativa Zea mays Triticum métabolisme activité enzymatique rhizosphère bactérie fixatrice de l'azote gène hydrogène oxygène Enterobacter Pseudomonas spectrométrie de masse Serratia Burkholderiaceae Stenotrophomonas http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_7950 http://aims.fao.org/aos/agrovoc/c_4769 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_6569 http://aims.fao.org/aos/agrovoc/c_27939 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_3724 http://aims.fao.org/aos/agrovoc/c_5477 http://aims.fao.org/aos/agrovoc/c_23918 http://aims.fao.org/aos/agrovoc/c_6304 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_14210 http://aims.fao.org/aos/agrovoc/c_37846 http://aims.fao.org/aos/agrovoc/c_3efe6f9c http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_8227 http://aims.fao.org/aos/agrovoc/c_2503 http://aims.fao.org/aos/agrovoc/c_6970 http://aims.fao.org/aos/agrovoc/c_4635 |
author |
Roumagnac, Philippe Richaud, Pierre Barakat, Mohamed Ortet, Philippe Roncato, Marie-Anne Heulin, Thierry Peltier, Gilles Achouak, Wafa Cournac, Laurent |
author_facet |
Roumagnac, Philippe Richaud, Pierre Barakat, Mohamed Ortet, Philippe Roncato, Marie-Anne Heulin, Thierry Peltier, Gilles Achouak, Wafa Cournac, Laurent |
author_sort |
Roumagnac, Philippe |
title |
Reversible oxygen-tolerant hydrogenase carried by free-living N2-fixing bacteria isolated from the rhizospheres of rice, maize, and wheat |
title_short |
Reversible oxygen-tolerant hydrogenase carried by free-living N2-fixing bacteria isolated from the rhizospheres of rice, maize, and wheat |
title_full |
Reversible oxygen-tolerant hydrogenase carried by free-living N2-fixing bacteria isolated from the rhizospheres of rice, maize, and wheat |
title_fullStr |
Reversible oxygen-tolerant hydrogenase carried by free-living N2-fixing bacteria isolated from the rhizospheres of rice, maize, and wheat |
title_full_unstemmed |
Reversible oxygen-tolerant hydrogenase carried by free-living N2-fixing bacteria isolated from the rhizospheres of rice, maize, and wheat |
title_sort |
reversible oxygen-tolerant hydrogenase carried by free-living n2-fixing bacteria isolated from the rhizospheres of rice, maize, and wheat |
url |
http://agritrop.cirad.fr/567103/ http://agritrop.cirad.fr/567103/1/document_567103.pdf |
work_keys_str_mv |
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