The direct protein-protein interaction results in the arms race co-evolution between Magnaporthe oryzae AVR-Pik and rice Pik

Between pathogen and host, antagonistic interactions impose strong reciprocal selection on each organism, leading to the development of arms race evolutionary dynamics. However, studies on specific recognition and co-evolution between resistance (R-) gene and avirulence (AVR-) gene are still limited. Here we show that AVRPik of Magnaporthe oryzae, the rice blast pathogen, and cognate rice R-gene Pik exhibit high levels of DNA polymorphisms causing amino acid changes. We found a tight recognition specificity of AVRPik alleles by different Pik alleles. Pik is composed of two kinds of CC-NBS-LRR, Pik1 and Pik2. We found that AVR-Pik physically interacts with the N-terminal coiled-coil domain of Pik1 in yeast 2-hybrid assay as well as in in-planta co-immunoprecipitation assay. Furthermore, this binding specificity corresponds to the recognition specificity between AVR-Pik and Pik alleles. These data suggest that the direct protein-protein interaction results in the arms race co-evolution between AVR-Pik and Pik. (Texte intégral)

Bibliographic Details

Main Authors:	Kanzaki, Hiroyuki, Yoshida, Kentaro, Saitoh, Hiromasa, Hirabuchi, Akiko, Alaux, Ludovic, Fournier, Elisabeth, Tharreau, Didier, Terauchi, Ryohei
Format:	conference_item biblioteca
Language:	eng
Published:	IS-MPMI
Subjects:	H20 - Maladies des plantes, F30 - Génétique et amélioration des plantes,
Online Access:	http://agritrop.cirad.fr/565196/ http://agritrop.cirad.fr/565196/1/document_565196.pdf
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