Symbiotic and molecular characterisation of the rhizobia associated with Acacia mangium x Acacia auriculiformis hybrids and both parental species
In the context of an increasing utilisation of the interspecific hybrid Acacia mangium x A. auriculiformis as a plantation tree for pulp production in Southeast Asia, the symbiotic characterisation of this hybrid was carried out in comparison with that of its both parental pure species. Rhizobium strains of diverse geographical origins were isolated from Acacia mangium, Acacia auriculiformis and the hybrid A. mangium x A. auriculiformis. Two types of strains could be distinguished according to their growth rate in pure culture, the Acacia hybrid strains having a fast growth conversely to the strains isolated from both parental species which had a far lower growth. The inoculation experiments performed in in vitro or greenhouse conditions showed that all rhizobium strains isolated from both parental species and their hybrid were infective and effective with these same three species, except one that formed unefficient nodules in A. mangium. However, although no specificity in strain infectivity was observed, they showed very variable effectivities according to the species tested. Thus, the strains isolated from the hybrid A. mangium x A. auriculiformis had a higher effectivity than strains isolated from A. mangium or A. auriculiformis when all these strains were inoculated to the Acacia hybrid. Conversely, the strains isolated from the hybrid A. mangium x A. auriculiformis generally exhibited a lower effectivity than A. mangium or A. auriculiformis strains when inoculated to each pure parental species. Then, the rhizobium strains were genetically characterised through partial sequencing of the intertranscript space region 16S-23S of the ribosomal DNA (ITS). The resulting phylogenetic tree showed that, except for few ones, all strains isolated from the hybrid A. mangium x A. auriculiformis were grouped within a same branch phylogenetically close to Bradyrhizobium japonicum reference strains whereas all strains isolated from A. mangium and A. auriculiformis, regardless of their geographic origin, were grouped in another branch including Bradyrhizobium elkanii reference strains. Since all the A. mangium x A. auriculiformis hybrid strains of our study originated from Malaysia, other ones from more diverse origins should be isolated and characterised in order to confirm this differentiation between them according to the hybrid or pure species status of the host plant of isolation. (Texte intégral)
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Subjects: | P34 - Biologie du sol, K10 - Production forestière, F62 - Physiologie végétale - Croissance et développement, Bradyrhizobium, Acacia auriculiformis, Acacia mangium, http://aims.fao.org/aos/agrovoc/c_27138, http://aims.fao.org/aos/agrovoc/c_35, http://aims.fao.org/aos/agrovoc/c_42, http://aims.fao.org/aos/agrovoc/c_4533, |
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dig-cirad-fr-5623082020-01-15T17:01:24Z http://agritrop.cirad.fr/562308/ http://agritrop.cirad.fr/562308/ Symbiotic and molecular characterisation of the rhizobia associated with Acacia mangium x Acacia auriculiformis hybrids and both parental species. Tentcheva Koleva Diana, Le Roux Christine, Prin Yves, Moo Hanna, Japarudin Yani, Galiana Antoine. 2004. In : 2nd Mediterranean Conference of Rhizobiology, Oran, Algeria, 23-25 May, 2004. s.l. : s.n., Résumé, 2 p, Mediterranean Conference of Rhizobiology. 2, Oran, Algérie, 23 Mai 2004/25 Mai 2004. Researchers Symbiotic and molecular characterisation of the rhizobia associated with Acacia mangium x Acacia auriculiformis hybrids and both parental species Tentcheva Koleva, Diana Le Roux, Christine Prin, Yves Moo, Hanna Japarudin, Yani Galiana, Antoine eng 2004 s.n. 2nd Mediterranean Conference of Rhizobiology, Oran, Algeria, 23-25 May, 2004 P34 - Biologie du sol K10 - Production forestière F62 - Physiologie végétale - Croissance et développement Bradyrhizobium Acacia auriculiformis Acacia mangium http://aims.fao.org/aos/agrovoc/c_27138 http://aims.fao.org/aos/agrovoc/c_35 http://aims.fao.org/aos/agrovoc/c_42 Malaisie http://aims.fao.org/aos/agrovoc/c_4533 In the context of an increasing utilisation of the interspecific hybrid Acacia mangium x A. auriculiformis as a plantation tree for pulp production in Southeast Asia, the symbiotic characterisation of this hybrid was carried out in comparison with that of its both parental pure species. Rhizobium strains of diverse geographical origins were isolated from Acacia mangium, Acacia auriculiformis and the hybrid A. mangium x A. auriculiformis. Two types of strains could be distinguished according to their growth rate in pure culture, the Acacia hybrid strains having a fast growth conversely to the strains isolated from both parental species which had a far lower growth. The inoculation experiments performed in in vitro or greenhouse conditions showed that all rhizobium strains isolated from both parental species and their hybrid were infective and effective with these same three species, except one that formed unefficient nodules in A. mangium. However, although no specificity in strain infectivity was observed, they showed very variable effectivities according to the species tested. Thus, the strains isolated from the hybrid A. mangium x A. auriculiformis had a higher effectivity than strains isolated from A. mangium or A. auriculiformis when all these strains were inoculated to the Acacia hybrid. Conversely, the strains isolated from the hybrid A. mangium x A. auriculiformis generally exhibited a lower effectivity than A. mangium or A. auriculiformis strains when inoculated to each pure parental species. Then, the rhizobium strains were genetically characterised through partial sequencing of the intertranscript space region 16S-23S of the ribosomal DNA (ITS). The resulting phylogenetic tree showed that, except for few ones, all strains isolated from the hybrid A. mangium x A. auriculiformis were grouped within a same branch phylogenetically close to Bradyrhizobium japonicum reference strains whereas all strains isolated from A. mangium and A. auriculiformis, regardless of their geographic origin, were grouped in another branch including Bradyrhizobium elkanii reference strains. Since all the A. mangium x A. auriculiformis hybrid strains of our study originated from Malaysia, other ones from more diverse origins should be isolated and characterised in order to confirm this differentiation between them according to the hybrid or pure species status of the host plant of isolation. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/562308/1/document_562308.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=213463 |
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P34 - Biologie du sol K10 - Production forestière F62 - Physiologie végétale - Croissance et développement Bradyrhizobium Acacia auriculiformis Acacia mangium http://aims.fao.org/aos/agrovoc/c_27138 http://aims.fao.org/aos/agrovoc/c_35 http://aims.fao.org/aos/agrovoc/c_42 http://aims.fao.org/aos/agrovoc/c_4533 P34 - Biologie du sol K10 - Production forestière F62 - Physiologie végétale - Croissance et développement Bradyrhizobium Acacia auriculiformis Acacia mangium http://aims.fao.org/aos/agrovoc/c_27138 http://aims.fao.org/aos/agrovoc/c_35 http://aims.fao.org/aos/agrovoc/c_42 http://aims.fao.org/aos/agrovoc/c_4533 |
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P34 - Biologie du sol K10 - Production forestière F62 - Physiologie végétale - Croissance et développement Bradyrhizobium Acacia auriculiformis Acacia mangium http://aims.fao.org/aos/agrovoc/c_27138 http://aims.fao.org/aos/agrovoc/c_35 http://aims.fao.org/aos/agrovoc/c_42 http://aims.fao.org/aos/agrovoc/c_4533 P34 - Biologie du sol K10 - Production forestière F62 - Physiologie végétale - Croissance et développement Bradyrhizobium Acacia auriculiformis Acacia mangium http://aims.fao.org/aos/agrovoc/c_27138 http://aims.fao.org/aos/agrovoc/c_35 http://aims.fao.org/aos/agrovoc/c_42 http://aims.fao.org/aos/agrovoc/c_4533 Tentcheva Koleva, Diana Le Roux, Christine Prin, Yves Moo, Hanna Japarudin, Yani Galiana, Antoine Symbiotic and molecular characterisation of the rhizobia associated with Acacia mangium x Acacia auriculiformis hybrids and both parental species |
description |
In the context of an increasing utilisation of the interspecific hybrid Acacia mangium x A. auriculiformis as a plantation tree for pulp production in Southeast Asia, the symbiotic characterisation of this hybrid was carried out in comparison with that of its both parental pure species. Rhizobium strains of diverse geographical origins were isolated from Acacia mangium, Acacia auriculiformis and the hybrid A. mangium x A. auriculiformis. Two types of strains could be distinguished according to their growth rate in pure culture, the Acacia hybrid strains having a fast growth conversely to the strains isolated from both parental species which had a far lower growth. The inoculation experiments performed in in vitro or greenhouse conditions showed that all rhizobium strains isolated from both parental species and their hybrid were infective and effective with these same three species, except one that formed unefficient nodules in A. mangium. However, although no specificity in strain infectivity was observed, they showed very variable effectivities according to the species tested. Thus, the strains isolated from the hybrid A. mangium x A. auriculiformis had a higher effectivity than strains isolated from A. mangium or A. auriculiformis when all these strains were inoculated to the Acacia hybrid. Conversely, the strains isolated from the hybrid A. mangium x A. auriculiformis generally exhibited a lower effectivity than A. mangium or A. auriculiformis strains when inoculated to each pure parental species. Then, the rhizobium strains were genetically characterised through partial sequencing of the intertranscript space region 16S-23S of the ribosomal DNA (ITS). The resulting phylogenetic tree showed that, except for few ones, all strains isolated from the hybrid A. mangium x A. auriculiformis were grouped within a same branch phylogenetically close to Bradyrhizobium japonicum reference strains whereas all strains isolated from A. mangium and A. auriculiformis, regardless of their geographic origin, were grouped in another branch including Bradyrhizobium elkanii reference strains. Since all the A. mangium x A. auriculiformis hybrid strains of our study originated from Malaysia, other ones from more diverse origins should be isolated and characterised in order to confirm this differentiation between them according to the hybrid or pure species status of the host plant of isolation. (Texte intégral) |
format |
conference_item |
topic_facet |
P34 - Biologie du sol K10 - Production forestière F62 - Physiologie végétale - Croissance et développement Bradyrhizobium Acacia auriculiformis Acacia mangium http://aims.fao.org/aos/agrovoc/c_27138 http://aims.fao.org/aos/agrovoc/c_35 http://aims.fao.org/aos/agrovoc/c_42 http://aims.fao.org/aos/agrovoc/c_4533 |
author |
Tentcheva Koleva, Diana Le Roux, Christine Prin, Yves Moo, Hanna Japarudin, Yani Galiana, Antoine |
author_facet |
Tentcheva Koleva, Diana Le Roux, Christine Prin, Yves Moo, Hanna Japarudin, Yani Galiana, Antoine |
author_sort |
Tentcheva Koleva, Diana |
title |
Symbiotic and molecular characterisation of the rhizobia associated with Acacia mangium x Acacia auriculiformis hybrids and both parental species |
title_short |
Symbiotic and molecular characterisation of the rhizobia associated with Acacia mangium x Acacia auriculiformis hybrids and both parental species |
title_full |
Symbiotic and molecular characterisation of the rhizobia associated with Acacia mangium x Acacia auriculiformis hybrids and both parental species |
title_fullStr |
Symbiotic and molecular characterisation of the rhizobia associated with Acacia mangium x Acacia auriculiformis hybrids and both parental species |
title_full_unstemmed |
Symbiotic and molecular characterisation of the rhizobia associated with Acacia mangium x Acacia auriculiformis hybrids and both parental species |
title_sort |
symbiotic and molecular characterisation of the rhizobia associated with acacia mangium x acacia auriculiformis hybrids and both parental species |
publisher |
s.n. |
url |
http://agritrop.cirad.fr/562308/ http://agritrop.cirad.fr/562308/1/document_562308.pdf |
work_keys_str_mv |
AT tentchevakolevadiana symbioticandmolecularcharacterisationoftherhizobiaassociatedwithacaciamangiumxacaciaauriculiformishybridsandbothparentalspecies AT lerouxchristine symbioticandmolecularcharacterisationoftherhizobiaassociatedwithacaciamangiumxacaciaauriculiformishybridsandbothparentalspecies AT prinyves symbioticandmolecularcharacterisationoftherhizobiaassociatedwithacaciamangiumxacaciaauriculiformishybridsandbothparentalspecies AT moohanna symbioticandmolecularcharacterisationoftherhizobiaassociatedwithacaciamangiumxacaciaauriculiformishybridsandbothparentalspecies AT japarudinyani symbioticandmolecularcharacterisationoftherhizobiaassociatedwithacaciamangiumxacaciaauriculiformishybridsandbothparentalspecies AT galianaantoine symbioticandmolecularcharacterisationoftherhizobiaassociatedwithacaciamangiumxacaciaauriculiformishybridsandbothparentalspecies |
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1758023096897372160 |