Variations of DNA methylation in Eucalyptus urophylla x Eucalyptus grandis shoot tips and apical meristems of different physiological ages
Global DNA methylation was assessed by high-performance liquid chromatography (HPLC) for the first time in Eucalyptus urophylla×Eucalyptus grandis shoot tips comparing three outdoor and one in vitro sources of related genotypes differing in their physiological age. The DNA methylation levels found were consistent with those reported for other Angiosperms using the same HPLC technology. Notwithstanding noticeable time-related fluctuations within each source of plant material, methylation rate was overall higher for the mature clone (13.7%) than for the rejuvenated line of the same clone (12.6%) and for the juvenile offspring seedlings (11.8%). The in vitro microshoots of the mature clone were less methylated (11.3%) than the other outdoor origins, but the difference with the juvenile seedlings was not significant. Immunofluorescence investigations on shoot apices established that the mature source could be distinguished from the rejuvenated and juvenile origins by a higher density of cells with methylated nuclei in leaf primordia. Shoot apical meristems (SAMs) from the mature clone also showed a greater proportion and more methylated cells than SAMs from the rejuvenated and juvenile origins. The nuclei of these latter were characterized by fewer and more dispersed labeled spots than for the mature source. Our findings establish that physiological ageing induced quantitative and qualitative variations of DNA methylation at shoot tip, SAM and even cellular levels. Overall this DNA methylation increased with maturation and conversely decreased with rejuvenation to reach the lower scores and to show the immunolabeling patterns that characterized juvenile material nuclei.
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Subjects: | F30 - Génétique et amélioration des plantes, F62 - Physiologie végétale - Croissance et développement, Eucalyptus urophylla, Eucalyptus grandis, http://aims.fao.org/aos/agrovoc/c_26492, http://aims.fao.org/aos/agrovoc/c_2693, http://aims.fao.org/aos/agrovoc/c_1811, |
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dig-cirad-fr-5610652022-12-30T18:33:41Z http://agritrop.cirad.fr/561065/ http://agritrop.cirad.fr/561065/ Variations of DNA methylation in Eucalyptus urophylla x Eucalyptus grandis shoot tips and apical meristems of different physiological ages. Mankessi François, Saya Aubin Rachel, Favreau Bénédicte, Doulbeau Sylvie, Conejero Geneviève, Lartaud Marc, Verdeil Jean-Luc, Monteuuis Olivier. 2011. Physiologia Plantarum, 143 (2) : 178-187.https://doi.org/10.1111/j.1399-3054.2011.01491.x <https://doi.org/10.1111/j.1399-3054.2011.01491.x> Researchers Variations of DNA methylation in Eucalyptus urophylla x Eucalyptus grandis shoot tips and apical meristems of different physiological ages Mankessi, François Saya, Aubin Rachel Favreau, Bénédicte Doulbeau, Sylvie Conejero, Geneviève Lartaud, Marc Verdeil, Jean-Luc Monteuuis, Olivier eng 2011 Physiologia Plantarum F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Eucalyptus urophylla Eucalyptus grandis http://aims.fao.org/aos/agrovoc/c_26492 http://aims.fao.org/aos/agrovoc/c_2693 Congo http://aims.fao.org/aos/agrovoc/c_1811 Global DNA methylation was assessed by high-performance liquid chromatography (HPLC) for the first time in Eucalyptus urophylla×Eucalyptus grandis shoot tips comparing three outdoor and one in vitro sources of related genotypes differing in their physiological age. The DNA methylation levels found were consistent with those reported for other Angiosperms using the same HPLC technology. Notwithstanding noticeable time-related fluctuations within each source of plant material, methylation rate was overall higher for the mature clone (13.7%) than for the rejuvenated line of the same clone (12.6%) and for the juvenile offspring seedlings (11.8%). The in vitro microshoots of the mature clone were less methylated (11.3%) than the other outdoor origins, but the difference with the juvenile seedlings was not significant. Immunofluorescence investigations on shoot apices established that the mature source could be distinguished from the rejuvenated and juvenile origins by a higher density of cells with methylated nuclei in leaf primordia. Shoot apical meristems (SAMs) from the mature clone also showed a greater proportion and more methylated cells than SAMs from the rejuvenated and juvenile origins. The nuclei of these latter were characterized by fewer and more dispersed labeled spots than for the mature source. Our findings establish that physiological ageing induced quantitative and qualitative variations of DNA methylation at shoot tip, SAM and even cellular levels. Overall this DNA methylation increased with maturation and conversely decreased with rejuvenation to reach the lower scores and to show the immunolabeling patterns that characterized juvenile material nuclei. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/561065/1/document_561065.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1111/j.1399-3054.2011.01491.x 10.1111/j.1399-3054.2011.01491.x info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1399-3054.2011.01491.x info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1111/j.1399-3054.2011.01491.x |
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F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Eucalyptus urophylla Eucalyptus grandis http://aims.fao.org/aos/agrovoc/c_26492 http://aims.fao.org/aos/agrovoc/c_2693 http://aims.fao.org/aos/agrovoc/c_1811 F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Eucalyptus urophylla Eucalyptus grandis http://aims.fao.org/aos/agrovoc/c_26492 http://aims.fao.org/aos/agrovoc/c_2693 http://aims.fao.org/aos/agrovoc/c_1811 |
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F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Eucalyptus urophylla Eucalyptus grandis http://aims.fao.org/aos/agrovoc/c_26492 http://aims.fao.org/aos/agrovoc/c_2693 http://aims.fao.org/aos/agrovoc/c_1811 F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Eucalyptus urophylla Eucalyptus grandis http://aims.fao.org/aos/agrovoc/c_26492 http://aims.fao.org/aos/agrovoc/c_2693 http://aims.fao.org/aos/agrovoc/c_1811 Mankessi, François Saya, Aubin Rachel Favreau, Bénédicte Doulbeau, Sylvie Conejero, Geneviève Lartaud, Marc Verdeil, Jean-Luc Monteuuis, Olivier Variations of DNA methylation in Eucalyptus urophylla x Eucalyptus grandis shoot tips and apical meristems of different physiological ages |
description |
Global DNA methylation was assessed by high-performance liquid chromatography (HPLC) for the first time in Eucalyptus urophylla×Eucalyptus grandis shoot tips comparing three outdoor and one in vitro sources of related genotypes differing in their physiological age. The DNA methylation levels found were consistent with those reported for other Angiosperms using the same HPLC technology. Notwithstanding noticeable time-related fluctuations within each source of plant material, methylation rate was overall higher for the mature clone (13.7%) than for the rejuvenated line of the same clone (12.6%) and for the juvenile offspring seedlings (11.8%). The in vitro microshoots of the mature clone were less methylated (11.3%) than the other outdoor origins, but the difference with the juvenile seedlings was not significant. Immunofluorescence investigations on shoot apices established that the mature source could be distinguished from the rejuvenated and juvenile origins by a higher density of cells with methylated nuclei in leaf primordia. Shoot apical meristems (SAMs) from the mature clone also showed a greater proportion and more methylated cells than SAMs from the rejuvenated and juvenile origins. The nuclei of these latter were characterized by fewer and more dispersed labeled spots than for the mature source. Our findings establish that physiological ageing induced quantitative and qualitative variations of DNA methylation at shoot tip, SAM and even cellular levels. Overall this DNA methylation increased with maturation and conversely decreased with rejuvenation to reach the lower scores and to show the immunolabeling patterns that characterized juvenile material nuclei. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Eucalyptus urophylla Eucalyptus grandis http://aims.fao.org/aos/agrovoc/c_26492 http://aims.fao.org/aos/agrovoc/c_2693 http://aims.fao.org/aos/agrovoc/c_1811 |
author |
Mankessi, François Saya, Aubin Rachel Favreau, Bénédicte Doulbeau, Sylvie Conejero, Geneviève Lartaud, Marc Verdeil, Jean-Luc Monteuuis, Olivier |
author_facet |
Mankessi, François Saya, Aubin Rachel Favreau, Bénédicte Doulbeau, Sylvie Conejero, Geneviève Lartaud, Marc Verdeil, Jean-Luc Monteuuis, Olivier |
author_sort |
Mankessi, François |
title |
Variations of DNA methylation in Eucalyptus urophylla x Eucalyptus grandis shoot tips and apical meristems of different physiological ages |
title_short |
Variations of DNA methylation in Eucalyptus urophylla x Eucalyptus grandis shoot tips and apical meristems of different physiological ages |
title_full |
Variations of DNA methylation in Eucalyptus urophylla x Eucalyptus grandis shoot tips and apical meristems of different physiological ages |
title_fullStr |
Variations of DNA methylation in Eucalyptus urophylla x Eucalyptus grandis shoot tips and apical meristems of different physiological ages |
title_full_unstemmed |
Variations of DNA methylation in Eucalyptus urophylla x Eucalyptus grandis shoot tips and apical meristems of different physiological ages |
title_sort |
variations of dna methylation in eucalyptus urophylla x eucalyptus grandis shoot tips and apical meristems of different physiological ages |
url |
http://agritrop.cirad.fr/561065/ http://agritrop.cirad.fr/561065/1/document_561065.pdf |
work_keys_str_mv |
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