Symbiont diversity and non-random hybridization among indigenous (Ms) and invasive (B) biotypes of Bemisia tabaci
The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is a worldwide pest and a vector of numerous plant viruses. B. tabaci is composed of dozens of morphologically indistinguishable biotypes and its taxonomic status is still controversial. This phloemfeeder harbours the primary symbiont Portiera aleyrodidarum and potentially six secondary symbionts: Cardinium, Arsenophonus, Hamiltonella, Rickettsia, Wolbachia and Fritschea. In the southwest Indian Ocean, La Re´union hosts two biotypes of this species: B (invasive) and Ms (indigenous). A multiplex PCR was developed to study the symbiont community of B. tabaci on La Re´union. Symbiont community prevalence and composition, host mitochondrial and nuclear genetic diversity, as well as host plant and localization, were described on field populations of La Re´union for B and Ms B. tabaci biotypes and their hybrids. A clear association between symbiotypes and biotypes was shown. Cardinium, Arsenophonus and Rickettsia were found in the Ms biotype (73.6%, 64.2% and 3.3%, respectively). Hamiltonella (exclusively) and Rickettsia were found in the B biotype (78% and 91.2%, respectively). Hybrids harboured all symbiotypes found in Ms and B populations, but with a higher prevalence of Ms symbiotypes than expected under random hybridization. An unexpected majority was Cardinium mono-infected (65.6%), and a striking minority (9%) harboured Cardinium ? Arsenophonus. In the hybrids only, genetic diversity was linked to symbiotype. Among the hybrids, significant links were found between symbiotypes and: (i) mitochondrial COI sequences, i.e. maternal origin; and (ii) alleles of nuclear microsatellite loci, specific to either Ms or B parental biotype. Taken together, our results suggest that Cardinium and ? or Arsenophonus may manipulate the reproduction of indigenous (Ms) with invasive (B) biotypes of Bemisia tabaci.
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Subjects: | H20 - Maladies des plantes, Bemisia tabaci, mauvaise herbe, plante de culture, http://aims.fao.org/aos/agrovoc/c_30755, http://aims.fao.org/aos/agrovoc/c_8347, http://aims.fao.org/aos/agrovoc/c_1972, http://aims.fao.org/aos/agrovoc/c_6543, http://aims.fao.org/aos/agrovoc/c_3081, |
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dig-cirad-fr-5596982024-01-28T19:09:32Z http://agritrop.cirad.fr/559698/ http://agritrop.cirad.fr/559698/ Symbiont diversity and non-random hybridization among indigenous (Ms) and invasive (B) biotypes of Bemisia tabaci. Thierry Magalie, Becker Nathalie, Hajri Ahmed, Reynaud Bernard, Lett Jean-Michel, Delatte Hélène. 2011. Molecular Ecology, 20 (10) : 2172-2187.https://doi.org/10.1111/j.1365-294X.2011.05087.x <https://doi.org/10.1111/j.1365-294X.2011.05087.x> Symbiont diversity and non-random hybridization among indigenous (Ms) and invasive (B) biotypes of Bemisia tabaci Thierry, Magalie Becker, Nathalie Hajri, Ahmed Reynaud, Bernard Lett, Jean-Michel Delatte, Hélène eng 2011 Molecular Ecology H20 - Maladies des plantes Bemisia tabaci mauvaise herbe plante de culture http://aims.fao.org/aos/agrovoc/c_30755 http://aims.fao.org/aos/agrovoc/c_8347 http://aims.fao.org/aos/agrovoc/c_1972 La Réunion France http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is a worldwide pest and a vector of numerous plant viruses. B. tabaci is composed of dozens of morphologically indistinguishable biotypes and its taxonomic status is still controversial. This phloemfeeder harbours the primary symbiont Portiera aleyrodidarum and potentially six secondary symbionts: Cardinium, Arsenophonus, Hamiltonella, Rickettsia, Wolbachia and Fritschea. In the southwest Indian Ocean, La Re´union hosts two biotypes of this species: B (invasive) and Ms (indigenous). A multiplex PCR was developed to study the symbiont community of B. tabaci on La Re´union. Symbiont community prevalence and composition, host mitochondrial and nuclear genetic diversity, as well as host plant and localization, were described on field populations of La Re´union for B and Ms B. tabaci biotypes and their hybrids. A clear association between symbiotypes and biotypes was shown. Cardinium, Arsenophonus and Rickettsia were found in the Ms biotype (73.6%, 64.2% and 3.3%, respectively). Hamiltonella (exclusively) and Rickettsia were found in the B biotype (78% and 91.2%, respectively). Hybrids harboured all symbiotypes found in Ms and B populations, but with a higher prevalence of Ms symbiotypes than expected under random hybridization. An unexpected majority was Cardinium mono-infected (65.6%), and a striking minority (9%) harboured Cardinium ? Arsenophonus. In the hybrids only, genetic diversity was linked to symbiotype. Among the hybrids, significant links were found between symbiotypes and: (i) mitochondrial COI sequences, i.e. maternal origin; and (ii) alleles of nuclear microsatellite loci, specific to either Ms or B parental biotype. Taken together, our results suggest that Cardinium and ? or Arsenophonus may manipulate the reproduction of indigenous (Ms) with invasive (B) biotypes of Bemisia tabaci. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/559698/1/document_559698.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1111/j.1365-294X.2011.05087.x 10.1111/j.1365-294X.2011.05087.x http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=211278 info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-294X.2011.05087.x info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1111/j.1365-294X.2011.05087.x |
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H20 - Maladies des plantes Bemisia tabaci mauvaise herbe plante de culture http://aims.fao.org/aos/agrovoc/c_30755 http://aims.fao.org/aos/agrovoc/c_8347 http://aims.fao.org/aos/agrovoc/c_1972 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes Bemisia tabaci mauvaise herbe plante de culture http://aims.fao.org/aos/agrovoc/c_30755 http://aims.fao.org/aos/agrovoc/c_8347 http://aims.fao.org/aos/agrovoc/c_1972 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 |
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H20 - Maladies des plantes Bemisia tabaci mauvaise herbe plante de culture http://aims.fao.org/aos/agrovoc/c_30755 http://aims.fao.org/aos/agrovoc/c_8347 http://aims.fao.org/aos/agrovoc/c_1972 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes Bemisia tabaci mauvaise herbe plante de culture http://aims.fao.org/aos/agrovoc/c_30755 http://aims.fao.org/aos/agrovoc/c_8347 http://aims.fao.org/aos/agrovoc/c_1972 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 Thierry, Magalie Becker, Nathalie Hajri, Ahmed Reynaud, Bernard Lett, Jean-Michel Delatte, Hélène Symbiont diversity and non-random hybridization among indigenous (Ms) and invasive (B) biotypes of Bemisia tabaci |
description |
The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is a worldwide pest and a vector of numerous plant viruses. B. tabaci is composed of dozens of morphologically indistinguishable biotypes and its taxonomic status is still controversial. This phloemfeeder harbours the primary symbiont Portiera aleyrodidarum and potentially six secondary symbionts: Cardinium, Arsenophonus, Hamiltonella, Rickettsia, Wolbachia and Fritschea. In the southwest Indian Ocean, La Re´union hosts two biotypes of this species: B (invasive) and Ms (indigenous). A multiplex PCR was developed to study the symbiont community of B. tabaci on La Re´union. Symbiont community prevalence and composition, host mitochondrial and nuclear genetic diversity, as well as host plant and localization, were described on field populations of La Re´union for B and Ms B. tabaci biotypes and their hybrids. A clear association between symbiotypes and biotypes was shown. Cardinium, Arsenophonus and Rickettsia were found in the Ms biotype (73.6%, 64.2% and 3.3%, respectively). Hamiltonella (exclusively) and Rickettsia were found in the B biotype (78% and 91.2%, respectively). Hybrids harboured all symbiotypes found in Ms and B populations, but with a higher prevalence of Ms symbiotypes than expected under random hybridization. An unexpected majority was Cardinium mono-infected (65.6%), and a striking minority (9%) harboured Cardinium ? Arsenophonus. In the hybrids only, genetic diversity was linked to symbiotype. Among the hybrids, significant links were found between symbiotypes and: (i) mitochondrial COI sequences, i.e. maternal origin; and (ii) alleles of nuclear microsatellite loci, specific to either Ms or B parental biotype. Taken together, our results suggest that Cardinium and ? or Arsenophonus may manipulate the reproduction of indigenous (Ms) with invasive (B) biotypes of Bemisia tabaci. |
format |
article |
topic_facet |
H20 - Maladies des plantes Bemisia tabaci mauvaise herbe plante de culture http://aims.fao.org/aos/agrovoc/c_30755 http://aims.fao.org/aos/agrovoc/c_8347 http://aims.fao.org/aos/agrovoc/c_1972 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Thierry, Magalie Becker, Nathalie Hajri, Ahmed Reynaud, Bernard Lett, Jean-Michel Delatte, Hélène |
author_facet |
Thierry, Magalie Becker, Nathalie Hajri, Ahmed Reynaud, Bernard Lett, Jean-Michel Delatte, Hélène |
author_sort |
Thierry, Magalie |
title |
Symbiont diversity and non-random hybridization among indigenous (Ms) and invasive (B) biotypes of Bemisia tabaci |
title_short |
Symbiont diversity and non-random hybridization among indigenous (Ms) and invasive (B) biotypes of Bemisia tabaci |
title_full |
Symbiont diversity and non-random hybridization among indigenous (Ms) and invasive (B) biotypes of Bemisia tabaci |
title_fullStr |
Symbiont diversity and non-random hybridization among indigenous (Ms) and invasive (B) biotypes of Bemisia tabaci |
title_full_unstemmed |
Symbiont diversity and non-random hybridization among indigenous (Ms) and invasive (B) biotypes of Bemisia tabaci |
title_sort |
symbiont diversity and non-random hybridization among indigenous (ms) and invasive (b) biotypes of bemisia tabaci |
url |
http://agritrop.cirad.fr/559698/ http://agritrop.cirad.fr/559698/1/document_559698.pdf |
work_keys_str_mv |
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1792497803297030144 |