Mapping, tagging and map-based cloning of simply inherited traits
Sugarcane is one of the most genetically complex species. It is both aneuploid and polyploid with a large number of chromosomes. Consequently, simply inherited traits are rare; only five have been identified to date of which four are disease-related (including rust resistance) and one is a morphological trait. The genetic complexity and large genome size of sugarcane, together with the Jack of a close diploid relative, make map-based gene cloning in sugarcane a challenge. This chapter outlines the strategies developed to overcome these difficulties and describes progress towards the cloning of a durable rust resistance gene. Using syntenic relationships with model crops (sorghum and rice), enriched BAC libraries and haplotypespecific chromosome walking, a high-density genetic map and a partial physical map of the region surrounding the rust resistance gene has been developed. A comparison with other homo(eo)logous chromosomes at this region reveals the presence of a large insertioncurrent efforts are focusd on characterizing and walking through the insertion.
| Main Authors: | , , |
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| Format: | book_section biblioteca |
| Language: | eng |
| Published: |
CRC Press
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| Subjects: | F30 - Génétique et amélioration des plantes, H20 - Maladies des plantes, Saccharum, Saccharum officinarum, Saccharum spontaneum, Mycovellosiella, Bipolaris, Puccinia melanocephala, http://aims.fao.org/aos/agrovoc/c_6725, http://aims.fao.org/aos/agrovoc/c_6727, http://aims.fao.org/aos/agrovoc/c_6729, http://aims.fao.org/aos/agrovoc/c_31585, http://aims.fao.org/aos/agrovoc/c_31546, http://aims.fao.org/aos/agrovoc/c_27331, |
| Online Access: | http://agritrop.cirad.fr/556707/ http://agritrop.cirad.fr/556707/1/ID556707.pdf |
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