An EST resource for tilapia based on 17 normalized libraries and assembly of 116,899 sequence tags
Background: Large collections of expressed sequence tags (ESTs) are a fundamental resource for analysis of gene expression and annotation of genome sequences. We generated 116,899 ESTs from 17 normalized and two non-normalized cDNA libraries representing 16 tissues from tilapia, a cichlid fish widely used in aquaculture and biological research. Results: The ESTs were assembled into 20,190 contigs and 36,028 singletons for a total of 56,218 unique sequences and a total assembled length of 35,168,415 bp. Over the whole project, a unique sequence was discovered for every 2.079 sequence reads. 17,722 (31.5%) of these unique sequences had significant BLAST hits (e-value < 10-10) to the UniProt database. Conclusion: Normalization of the cDNA pools with double-stranded nuclease allowed us to efficiently sequence a large collection of ESTs. These sequences are an important resource for studies of gene expression, comparative mapping and annotation of the forthcoming tilapia genome sequence.
| Main Authors: | , , , , , , , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | L10 - Génétique et amélioration des animaux, M12 - Production de l'aquaculture, tilapia (genre), séquence nucléotidique, ADN recombiné, expression des gènes, génome, http://aims.fao.org/aos/agrovoc/c_7768, http://aims.fao.org/aos/agrovoc/c_27583, http://aims.fao.org/aos/agrovoc/c_27594, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_3224, |
| Online Access: | http://agritrop.cirad.fr/556389/ http://agritrop.cirad.fr/556389/1/document_556389.pdf |
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