Amplified fragment length polymorphism and multilocus sequence analysis-based genotypic relatedness among pathogenic variants of Xanthomonas citri pv. citri and Xanthomonas campestris pv. bilvae
Three pathogenic variants (i.e. pathotypes) have been described within Xanthomonas citri pv. citri, the causal agent of Asiatic citrus canker. Pathotype A strains naturally infect a wide range of Citrus species and members of some related genera. In contrast, pathotypes A* and Aw have narrow host ranges within the genus Citrus and have been isolated from Mexican lime (Citrus aurantifolia L.) and from Mexican lime and alemow (Citrus macrophylla L.), respectively. We used amplified fragment length polymorphism (AFLP) and multilocus sequence analysis (MLSA) based on four partial housekeeping gene sequences (atpD, dnaK, efp and gyrB) for the genotypic classification of Xanthomonas citri pv. citri and the poorly characterized citrus pathogen Xanthomonas campestris pv. bilvae. A Mantel test showed that genetic distances derived from AFLP and MLSA were highly correlated. X. campestris pv. bilvae showed a close relatedness to the type strain of X. citri, indicating that this pathovar should be reclassified as X. citri pv. bilvae. All pathotype A* and Aw strains were most closely related to X. citri pv. citri strains with a wide host range (pathotype A), confirming previous DNA¿DNA hybridization data. Pathotype Aw should be considered a junior synonym of pathotype A* on the basis of pathogenicity tests, AFLP, MLSA and PCR using pathovar-specific primers. Evolutionary genome divergences computed from AFLP data suggested that pathotype A* (including Aw strains) is a group of strains that shows a wider genetic diversity than pathotype A.
Main Authors: | , , , , , , , |
---|---|
Format: | article biblioteca |
Language: | eng |
Subjects: | H20 - Maladies des plantes, Citrus, Citrus paradisi, Citrus macrophylla, Citrus aurantiifolia, Xanthomonas, Xanthomonas campestris, Xanthomonas campestris citri, http://aims.fao.org/aos/agrovoc/c_1637, http://aims.fao.org/aos/agrovoc/c_1645, http://aims.fao.org/aos/agrovoc/c_35537, http://aims.fao.org/aos/agrovoc/c_1638, http://aims.fao.org/aos/agrovoc/c_8455, http://aims.fao.org/aos/agrovoc/c_24379, http://aims.fao.org/aos/agrovoc/c_36120, http://aims.fao.org/aos/agrovoc/c_6543, http://aims.fao.org/aos/agrovoc/c_3081, |
Online Access: | http://agritrop.cirad.fr/554152/ http://agritrop.cirad.fr/554152/1/document_554152.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-554152 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5541522024-01-28T18:15:46Z http://agritrop.cirad.fr/554152/ http://agritrop.cirad.fr/554152/ Amplified fragment length polymorphism and multilocus sequence analysis-based genotypic relatedness among pathogenic variants of Xanthomonas citri pv. citri and Xanthomonas campestris pv. bilvae. Bui Thi Ngoc Lan, Vernière Christian, Jouen Emmanuel, Ah-You Nathalie, Lefeuvre Pierre, Chiroleu Frédéric, Gagnevin Lionel, Pruvost Olivier. 2010. International Journal of Systematic and Evolutionary Microbiology, 60 (3) : 515-525.https://doi.org/10.1099/ijs.0.009514-0 <https://doi.org/10.1099/ijs.0.009514-0> Amplified fragment length polymorphism and multilocus sequence analysis-based genotypic relatedness among pathogenic variants of Xanthomonas citri pv. citri and Xanthomonas campestris pv. bilvae Bui Thi Ngoc, Lan Vernière, Christian Jouen, Emmanuel Ah-You, Nathalie Lefeuvre, Pierre Chiroleu, Frédéric Gagnevin, Lionel Pruvost, Olivier eng 2010 International Journal of Systematic and Evolutionary Microbiology H20 - Maladies des plantes Citrus Citrus paradisi Citrus macrophylla Citrus aurantiifolia Xanthomonas Xanthomonas campestris Xanthomonas campestris citri http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_1645 http://aims.fao.org/aos/agrovoc/c_35537 http://aims.fao.org/aos/agrovoc/c_1638 http://aims.fao.org/aos/agrovoc/c_8455 http://aims.fao.org/aos/agrovoc/c_24379 http://aims.fao.org/aos/agrovoc/c_36120 La Réunion France http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 Three pathogenic variants (i.e. pathotypes) have been described within Xanthomonas citri pv. citri, the causal agent of Asiatic citrus canker. Pathotype A strains naturally infect a wide range of Citrus species and members of some related genera. In contrast, pathotypes A* and Aw have narrow host ranges within the genus Citrus and have been isolated from Mexican lime (Citrus aurantifolia L.) and from Mexican lime and alemow (Citrus macrophylla L.), respectively. We used amplified fragment length polymorphism (AFLP) and multilocus sequence analysis (MLSA) based on four partial housekeeping gene sequences (atpD, dnaK, efp and gyrB) for the genotypic classification of Xanthomonas citri pv. citri and the poorly characterized citrus pathogen Xanthomonas campestris pv. bilvae. A Mantel test showed that genetic distances derived from AFLP and MLSA were highly correlated. X. campestris pv. bilvae showed a close relatedness to the type strain of X. citri, indicating that this pathovar should be reclassified as X. citri pv. bilvae. All pathotype A* and Aw strains were most closely related to X. citri pv. citri strains with a wide host range (pathotype A), confirming previous DNA¿DNA hybridization data. Pathotype Aw should be considered a junior synonym of pathotype A* on the basis of pathogenicity tests, AFLP, MLSA and PCR using pathovar-specific primers. Evolutionary genome divergences computed from AFLP data suggested that pathotype A* (including Aw strains) is a group of strains that shows a wider genetic diversity than pathotype A. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/554152/1/document_554152.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1099/ijs.0.009514-0 10.1099/ijs.0.009514-0 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=207726 info:eu-repo/semantics/altIdentifier/doi/10.1099/ijs.0.009514-0 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1099/ijs.0.009514-0 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
H20 - Maladies des plantes Citrus Citrus paradisi Citrus macrophylla Citrus aurantiifolia Xanthomonas Xanthomonas campestris Xanthomonas campestris citri http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_1645 http://aims.fao.org/aos/agrovoc/c_35537 http://aims.fao.org/aos/agrovoc/c_1638 http://aims.fao.org/aos/agrovoc/c_8455 http://aims.fao.org/aos/agrovoc/c_24379 http://aims.fao.org/aos/agrovoc/c_36120 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes Citrus Citrus paradisi Citrus macrophylla Citrus aurantiifolia Xanthomonas Xanthomonas campestris Xanthomonas campestris citri http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_1645 http://aims.fao.org/aos/agrovoc/c_35537 http://aims.fao.org/aos/agrovoc/c_1638 http://aims.fao.org/aos/agrovoc/c_8455 http://aims.fao.org/aos/agrovoc/c_24379 http://aims.fao.org/aos/agrovoc/c_36120 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 |
spellingShingle |
H20 - Maladies des plantes Citrus Citrus paradisi Citrus macrophylla Citrus aurantiifolia Xanthomonas Xanthomonas campestris Xanthomonas campestris citri http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_1645 http://aims.fao.org/aos/agrovoc/c_35537 http://aims.fao.org/aos/agrovoc/c_1638 http://aims.fao.org/aos/agrovoc/c_8455 http://aims.fao.org/aos/agrovoc/c_24379 http://aims.fao.org/aos/agrovoc/c_36120 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes Citrus Citrus paradisi Citrus macrophylla Citrus aurantiifolia Xanthomonas Xanthomonas campestris Xanthomonas campestris citri http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_1645 http://aims.fao.org/aos/agrovoc/c_35537 http://aims.fao.org/aos/agrovoc/c_1638 http://aims.fao.org/aos/agrovoc/c_8455 http://aims.fao.org/aos/agrovoc/c_24379 http://aims.fao.org/aos/agrovoc/c_36120 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 Bui Thi Ngoc, Lan Vernière, Christian Jouen, Emmanuel Ah-You, Nathalie Lefeuvre, Pierre Chiroleu, Frédéric Gagnevin, Lionel Pruvost, Olivier Amplified fragment length polymorphism and multilocus sequence analysis-based genotypic relatedness among pathogenic variants of Xanthomonas citri pv. citri and Xanthomonas campestris pv. bilvae |
description |
Three pathogenic variants (i.e. pathotypes) have been described within Xanthomonas citri pv. citri, the causal agent of Asiatic citrus canker. Pathotype A strains naturally infect a wide range of Citrus species and members of some related genera. In contrast, pathotypes A* and Aw have narrow host ranges within the genus Citrus and have been isolated from Mexican lime (Citrus aurantifolia L.) and from Mexican lime and alemow (Citrus macrophylla L.), respectively. We used amplified fragment length polymorphism (AFLP) and multilocus sequence analysis (MLSA) based on four partial housekeeping gene sequences (atpD, dnaK, efp and gyrB) for the genotypic classification of Xanthomonas citri pv. citri and the poorly characterized citrus pathogen Xanthomonas campestris pv. bilvae. A Mantel test showed that genetic distances derived from AFLP and MLSA were highly correlated. X. campestris pv. bilvae showed a close relatedness to the type strain of X. citri, indicating that this pathovar should be reclassified as X. citri pv. bilvae. All pathotype A* and Aw strains were most closely related to X. citri pv. citri strains with a wide host range (pathotype A), confirming previous DNA¿DNA hybridization data. Pathotype Aw should be considered a junior synonym of pathotype A* on the basis of pathogenicity tests, AFLP, MLSA and PCR using pathovar-specific primers. Evolutionary genome divergences computed from AFLP data suggested that pathotype A* (including Aw strains) is a group of strains that shows a wider genetic diversity than pathotype A. |
format |
article |
topic_facet |
H20 - Maladies des plantes Citrus Citrus paradisi Citrus macrophylla Citrus aurantiifolia Xanthomonas Xanthomonas campestris Xanthomonas campestris citri http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_1645 http://aims.fao.org/aos/agrovoc/c_35537 http://aims.fao.org/aos/agrovoc/c_1638 http://aims.fao.org/aos/agrovoc/c_8455 http://aims.fao.org/aos/agrovoc/c_24379 http://aims.fao.org/aos/agrovoc/c_36120 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Bui Thi Ngoc, Lan Vernière, Christian Jouen, Emmanuel Ah-You, Nathalie Lefeuvre, Pierre Chiroleu, Frédéric Gagnevin, Lionel Pruvost, Olivier |
author_facet |
Bui Thi Ngoc, Lan Vernière, Christian Jouen, Emmanuel Ah-You, Nathalie Lefeuvre, Pierre Chiroleu, Frédéric Gagnevin, Lionel Pruvost, Olivier |
author_sort |
Bui Thi Ngoc, Lan |
title |
Amplified fragment length polymorphism and multilocus sequence analysis-based genotypic relatedness among pathogenic variants of Xanthomonas citri pv. citri and Xanthomonas campestris pv. bilvae |
title_short |
Amplified fragment length polymorphism and multilocus sequence analysis-based genotypic relatedness among pathogenic variants of Xanthomonas citri pv. citri and Xanthomonas campestris pv. bilvae |
title_full |
Amplified fragment length polymorphism and multilocus sequence analysis-based genotypic relatedness among pathogenic variants of Xanthomonas citri pv. citri and Xanthomonas campestris pv. bilvae |
title_fullStr |
Amplified fragment length polymorphism and multilocus sequence analysis-based genotypic relatedness among pathogenic variants of Xanthomonas citri pv. citri and Xanthomonas campestris pv. bilvae |
title_full_unstemmed |
Amplified fragment length polymorphism and multilocus sequence analysis-based genotypic relatedness among pathogenic variants of Xanthomonas citri pv. citri and Xanthomonas campestris pv. bilvae |
title_sort |
amplified fragment length polymorphism and multilocus sequence analysis-based genotypic relatedness among pathogenic variants of xanthomonas citri pv. citri and xanthomonas campestris pv. bilvae |
url |
http://agritrop.cirad.fr/554152/ http://agritrop.cirad.fr/554152/1/document_554152.pdf |
work_keys_str_mv |
AT buithingoclan amplifiedfragmentlengthpolymorphismandmultilocussequenceanalysisbasedgenotypicrelatednessamongpathogenicvariantsofxanthomonascitripvcitriandxanthomonascampestrispvbilvae AT vernierechristian amplifiedfragmentlengthpolymorphismandmultilocussequenceanalysisbasedgenotypicrelatednessamongpathogenicvariantsofxanthomonascitripvcitriandxanthomonascampestrispvbilvae AT jouenemmanuel amplifiedfragmentlengthpolymorphismandmultilocussequenceanalysisbasedgenotypicrelatednessamongpathogenicvariantsofxanthomonascitripvcitriandxanthomonascampestrispvbilvae AT ahyounathalie amplifiedfragmentlengthpolymorphismandmultilocussequenceanalysisbasedgenotypicrelatednessamongpathogenicvariantsofxanthomonascitripvcitriandxanthomonascampestrispvbilvae AT lefeuvrepierre amplifiedfragmentlengthpolymorphismandmultilocussequenceanalysisbasedgenotypicrelatednessamongpathogenicvariantsofxanthomonascitripvcitriandxanthomonascampestrispvbilvae AT chiroleufrederic amplifiedfragmentlengthpolymorphismandmultilocussequenceanalysisbasedgenotypicrelatednessamongpathogenicvariantsofxanthomonascitripvcitriandxanthomonascampestrispvbilvae AT gagnevinlionel amplifiedfragmentlengthpolymorphismandmultilocussequenceanalysisbasedgenotypicrelatednessamongpathogenicvariantsofxanthomonascitripvcitriandxanthomonascampestrispvbilvae AT pruvostolivier amplifiedfragmentlengthpolymorphismandmultilocussequenceanalysisbasedgenotypicrelatednessamongpathogenicvariantsofxanthomonascitripvcitriandxanthomonascampestrispvbilvae |
_version_ |
1792497542833897472 |