Analysis of cellular responses to Mycoplasma mycoides subsp. mycoides small colony biotype associated with control of contagious bovine pleuropneumonia
A better understanding of protective immune memory against contagious bovine pleuropneumonia (CBPP) is needed in order to facilitate the development of safer vaccines based on selected components of the pathogen. For this purpose, cells collected from lymph nodes draining the lungs of Mycoplasma mycoides subsp. mycoides small colony biotype (MmmSC)-infected cattle were stimulated with the pathogen in vitro and evaluated concurrently for proliferation (CFSE based method), expression of activation, memory markers and cytokine production. Direct evidence is presented for a major contribution of CD4+T cells to the vigorous proliferative and T1 biased cytokine recall responses observed in cattle that have recovered from infection but not in animals developing the acute form of the disease. Two different phenotypes of MmmSC-specific memory CD4 were observed based on CD62L expression and proliferative capacities. Furthermore, recall proliferation of B cells also occurred but was strictly dependent on the presence of CD4. The information provided in this study will facilitate the search for MmmSC antigens that have potential for the development of subunit vaccines against CBPP.
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Subjects: | L73 - Maladies des animaux, L72 - Organismes nuisibles des animaux, Mycoplasma mycoides, péripneumonie contagieuse bovine, vaccin, lymphocyte, immunologie, http://aims.fao.org/aos/agrovoc/c_23951, http://aims.fao.org/aos/agrovoc/c_16706, http://aims.fao.org/aos/agrovoc/c_8130, http://aims.fao.org/aos/agrovoc/c_4484, http://aims.fao.org/aos/agrovoc/c_3808, |
Online Access: | http://agritrop.cirad.fr/543267/ http://agritrop.cirad.fr/543267/1/document_543267.pdf |
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dig-cirad-fr-5432672024-01-28T15:38:56Z http://agritrop.cirad.fr/543267/ http://agritrop.cirad.fr/543267/ Analysis of cellular responses to Mycoplasma mycoides subsp. mycoides small colony biotype associated with control of contagious bovine pleuropneumonia. Totté Philippe, Rodrigues Valérie, YaYa Aboubakar, Hamadou B., Cissé Ousmane, Diallo Mahamadou, Niang Mamadou, Thiaucourt François, Dedieu Laurence. 2008. Veterinary Research, 39 (1) : 1-11.https://doi.org/10.1051/vetres:2007046 <https://doi.org/10.1051/vetres:2007046> Analysis of cellular responses to Mycoplasma mycoides subsp. mycoides small colony biotype associated with control of contagious bovine pleuropneumonia Totté, Philippe Rodrigues, Valérie YaYa, Aboubakar Hamadou, B. Cissé, Ousmane Diallo, Mahamadou Niang, Mamadou Thiaucourt, François Dedieu, Laurence eng 2008 Veterinary Research L73 - Maladies des animaux L72 - Organismes nuisibles des animaux Mycoplasma mycoides péripneumonie contagieuse bovine vaccin lymphocyte immunologie http://aims.fao.org/aos/agrovoc/c_23951 http://aims.fao.org/aos/agrovoc/c_16706 http://aims.fao.org/aos/agrovoc/c_8130 http://aims.fao.org/aos/agrovoc/c_4484 http://aims.fao.org/aos/agrovoc/c_3808 A better understanding of protective immune memory against contagious bovine pleuropneumonia (CBPP) is needed in order to facilitate the development of safer vaccines based on selected components of the pathogen. For this purpose, cells collected from lymph nodes draining the lungs of Mycoplasma mycoides subsp. mycoides small colony biotype (MmmSC)-infected cattle were stimulated with the pathogen in vitro and evaluated concurrently for proliferation (CFSE based method), expression of activation, memory markers and cytokine production. Direct evidence is presented for a major contribution of CD4+T cells to the vigorous proliferative and T1 biased cytokine recall responses observed in cattle that have recovered from infection but not in animals developing the acute form of the disease. Two different phenotypes of MmmSC-specific memory CD4 were observed based on CD62L expression and proliferative capacities. Furthermore, recall proliferation of B cells also occurred but was strictly dependent on the presence of CD4. The information provided in this study will facilitate the search for MmmSC antigens that have potential for the development of subunit vaccines against CBPP. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/543267/1/document_543267.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1051/vetres:2007046 10.1051/vetres:2007046 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=199662 info:eu-repo/semantics/altIdentifier/doi/10.1051/vetres:2007046 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1051/vetres:2007046 |
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L73 - Maladies des animaux L72 - Organismes nuisibles des animaux Mycoplasma mycoides péripneumonie contagieuse bovine vaccin lymphocyte immunologie http://aims.fao.org/aos/agrovoc/c_23951 http://aims.fao.org/aos/agrovoc/c_16706 http://aims.fao.org/aos/agrovoc/c_8130 http://aims.fao.org/aos/agrovoc/c_4484 http://aims.fao.org/aos/agrovoc/c_3808 L73 - Maladies des animaux L72 - Organismes nuisibles des animaux Mycoplasma mycoides péripneumonie contagieuse bovine vaccin lymphocyte immunologie http://aims.fao.org/aos/agrovoc/c_23951 http://aims.fao.org/aos/agrovoc/c_16706 http://aims.fao.org/aos/agrovoc/c_8130 http://aims.fao.org/aos/agrovoc/c_4484 http://aims.fao.org/aos/agrovoc/c_3808 |
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L73 - Maladies des animaux L72 - Organismes nuisibles des animaux Mycoplasma mycoides péripneumonie contagieuse bovine vaccin lymphocyte immunologie http://aims.fao.org/aos/agrovoc/c_23951 http://aims.fao.org/aos/agrovoc/c_16706 http://aims.fao.org/aos/agrovoc/c_8130 http://aims.fao.org/aos/agrovoc/c_4484 http://aims.fao.org/aos/agrovoc/c_3808 L73 - Maladies des animaux L72 - Organismes nuisibles des animaux Mycoplasma mycoides péripneumonie contagieuse bovine vaccin lymphocyte immunologie http://aims.fao.org/aos/agrovoc/c_23951 http://aims.fao.org/aos/agrovoc/c_16706 http://aims.fao.org/aos/agrovoc/c_8130 http://aims.fao.org/aos/agrovoc/c_4484 http://aims.fao.org/aos/agrovoc/c_3808 Totté, Philippe Rodrigues, Valérie YaYa, Aboubakar Hamadou, B. Cissé, Ousmane Diallo, Mahamadou Niang, Mamadou Thiaucourt, François Dedieu, Laurence Analysis of cellular responses to Mycoplasma mycoides subsp. mycoides small colony biotype associated with control of contagious bovine pleuropneumonia |
description |
A better understanding of protective immune memory against contagious bovine pleuropneumonia (CBPP) is needed in order to facilitate the development of safer vaccines based on selected components of the pathogen. For this purpose, cells collected from lymph nodes draining the lungs of Mycoplasma mycoides subsp. mycoides small colony biotype (MmmSC)-infected cattle were stimulated with the pathogen in vitro and evaluated concurrently for proliferation (CFSE based method), expression of activation, memory markers and cytokine production. Direct evidence is presented for a major contribution of CD4+T cells to the vigorous proliferative and T1 biased cytokine recall responses observed in cattle that have recovered from infection but not in animals developing the acute form of the disease. Two different phenotypes of MmmSC-specific memory CD4 were observed based on CD62L expression and proliferative capacities. Furthermore, recall proliferation of B cells also occurred but was strictly dependent on the presence of CD4. The information provided in this study will facilitate the search for MmmSC antigens that have potential for the development of subunit vaccines against CBPP. |
format |
article |
topic_facet |
L73 - Maladies des animaux L72 - Organismes nuisibles des animaux Mycoplasma mycoides péripneumonie contagieuse bovine vaccin lymphocyte immunologie http://aims.fao.org/aos/agrovoc/c_23951 http://aims.fao.org/aos/agrovoc/c_16706 http://aims.fao.org/aos/agrovoc/c_8130 http://aims.fao.org/aos/agrovoc/c_4484 http://aims.fao.org/aos/agrovoc/c_3808 |
author |
Totté, Philippe Rodrigues, Valérie YaYa, Aboubakar Hamadou, B. Cissé, Ousmane Diallo, Mahamadou Niang, Mamadou Thiaucourt, François Dedieu, Laurence |
author_facet |
Totté, Philippe Rodrigues, Valérie YaYa, Aboubakar Hamadou, B. Cissé, Ousmane Diallo, Mahamadou Niang, Mamadou Thiaucourt, François Dedieu, Laurence |
author_sort |
Totté, Philippe |
title |
Analysis of cellular responses to Mycoplasma mycoides subsp. mycoides small colony biotype associated with control of contagious bovine pleuropneumonia |
title_short |
Analysis of cellular responses to Mycoplasma mycoides subsp. mycoides small colony biotype associated with control of contagious bovine pleuropneumonia |
title_full |
Analysis of cellular responses to Mycoplasma mycoides subsp. mycoides small colony biotype associated with control of contagious bovine pleuropneumonia |
title_fullStr |
Analysis of cellular responses to Mycoplasma mycoides subsp. mycoides small colony biotype associated with control of contagious bovine pleuropneumonia |
title_full_unstemmed |
Analysis of cellular responses to Mycoplasma mycoides subsp. mycoides small colony biotype associated with control of contagious bovine pleuropneumonia |
title_sort |
analysis of cellular responses to mycoplasma mycoides subsp. mycoides small colony biotype associated with control of contagious bovine pleuropneumonia |
url |
http://agritrop.cirad.fr/543267/ http://agritrop.cirad.fr/543267/1/document_543267.pdf |
work_keys_str_mv |
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1792496800388612096 |