Trypanosoma congolense heat-shock protein 70 : a promising TH1 adjuvant
Developing immunopotentiators to improve vaccine efficacy in ruminants is an important goal. Heat shock proteins (HSPs) have been shown to act as adjuvant when co-administrated with peptides antigens or given as fusion proteins. In this study, we have investigated the role of Trypanosoma congolense HSP-70 (Tcong-HSP-70) on the maturation of murine dendritic cells (DCs) generated from bone marrow precursor cells. The results obtained showed that Tcong-HSP-70 induced murine DC maturation as determined by increased levels of surface markers (CD40, CD86 and MHC II) as well as proliferation of allogeneic splenocytes. DCs exposed to Tcong-HSP-70 were characterized by strong expression of TNF[alpha] and IL-12p70 and moderate expression of IL-10. Similar study, now underway, on bovine monocyte-derived DCs demonstrated the ability of Tcong-HSP-70 to also trigger bovine Mo-DC maturation. This finding thus suggests that Tcong-HSP-70 can act as a potent Th1 adjuvant for antigen delivery.
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dig-cirad-fr-5415722019-05-28T16:01:36Z http://agritrop.cirad.fr/541572/ http://agritrop.cirad.fr/541572/ Trypanosoma congolense heat-shock protein 70 : a promising TH1 adjuvant. Garzon Edwin, Bossard Géraldine, Bras R., Totté Philippe, Rodrigues Valérie, Boulanger A., Authié Edith, Dedieu Laurence. 2007. In : Does control of animal infectious risks offer a new international perspective ? : proceedings of the 12th International Conference of the Association of Institutions of Tropical Veterinary Medicine, Montpellier, France, 20-22 August 2007. Camus Emmanuel (ed.), Cardinale Eric (ed.), Dalibard Christophe (ed.), Martinez Dominique (ed.), Renard Jean-François (ed.), Roger François (ed.). CIRAD. Montpellier : CIRAD, 201-204. ISBN 978-2-87614-650-1 International Conference of the Association of Institutions of Tropical Veterinary Medicine. 12, Montpellier, France, 20 Août 2007/22 Août 2007. Researchers Trypanosoma congolense heat-shock protein 70 : a promising TH1 adjuvant Garzon, Edwin Bossard, Géraldine Bras, R. Totté, Philippe Rodrigues, Valérie Boulanger, A. Authié, Edith Dedieu, Laurence eng 2007 CIRAD Does control of animal infectious risks offer a new international perspective ? : proceedings of the 12th International Conference of the Association of Institutions of Tropical Veterinary Medicine, Montpellier, France, 20-22 August 2007 L72 - Organismes nuisibles des animaux Developing immunopotentiators to improve vaccine efficacy in ruminants is an important goal. Heat shock proteins (HSPs) have been shown to act as adjuvant when co-administrated with peptides antigens or given as fusion proteins. In this study, we have investigated the role of Trypanosoma congolense HSP-70 (Tcong-HSP-70) on the maturation of murine dendritic cells (DCs) generated from bone marrow precursor cells. The results obtained showed that Tcong-HSP-70 induced murine DC maturation as determined by increased levels of surface markers (CD40, CD86 and MHC II) as well as proliferation of allogeneic splenocytes. DCs exposed to Tcong-HSP-70 were characterized by strong expression of TNF[alpha] and IL-12p70 and moderate expression of IL-10. Similar study, now underway, on bovine monocyte-derived DCs demonstrated the ability of Tcong-HSP-70 to also trigger bovine Mo-DC maturation. This finding thus suggests that Tcong-HSP-70 can act as a potent Th1 adjuvant for antigen delivery. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/541572/1/ID541572.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html http://agritrop.cirad.fr/541504/ |
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L72 - Organismes nuisibles des animaux L72 - Organismes nuisibles des animaux Garzon, Edwin Bossard, Géraldine Bras, R. Totté, Philippe Rodrigues, Valérie Boulanger, A. Authié, Edith Dedieu, Laurence Trypanosoma congolense heat-shock protein 70 : a promising TH1 adjuvant |
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Developing immunopotentiators to improve vaccine efficacy in ruminants is an important goal. Heat shock proteins (HSPs) have been shown to act as adjuvant when co-administrated with peptides antigens or given as fusion proteins. In this study, we have investigated the role of Trypanosoma congolense HSP-70 (Tcong-HSP-70) on the maturation of murine dendritic cells (DCs) generated from bone marrow precursor cells. The results obtained showed that Tcong-HSP-70 induced murine DC maturation as determined by increased levels of surface markers (CD40, CD86 and MHC II) as well as proliferation of allogeneic splenocytes. DCs exposed to Tcong-HSP-70 were characterized by strong expression of TNF[alpha] and IL-12p70 and moderate expression of IL-10. Similar study, now underway, on bovine monocyte-derived DCs demonstrated the ability of Tcong-HSP-70 to also trigger bovine Mo-DC maturation. This finding thus suggests that Tcong-HSP-70 can act as a potent Th1 adjuvant for antigen delivery. |
format |
conference_item |
topic_facet |
L72 - Organismes nuisibles des animaux |
author |
Garzon, Edwin Bossard, Géraldine Bras, R. Totté, Philippe Rodrigues, Valérie Boulanger, A. Authié, Edith Dedieu, Laurence |
author_facet |
Garzon, Edwin Bossard, Géraldine Bras, R. Totté, Philippe Rodrigues, Valérie Boulanger, A. Authié, Edith Dedieu, Laurence |
author_sort |
Garzon, Edwin |
title |
Trypanosoma congolense heat-shock protein 70 : a promising TH1 adjuvant |
title_short |
Trypanosoma congolense heat-shock protein 70 : a promising TH1 adjuvant |
title_full |
Trypanosoma congolense heat-shock protein 70 : a promising TH1 adjuvant |
title_fullStr |
Trypanosoma congolense heat-shock protein 70 : a promising TH1 adjuvant |
title_full_unstemmed |
Trypanosoma congolense heat-shock protein 70 : a promising TH1 adjuvant |
title_sort |
trypanosoma congolense heat-shock protein 70 : a promising th1 adjuvant |
publisher |
CIRAD |
url |
http://agritrop.cirad.fr/541572/ http://agritrop.cirad.fr/541572/1/ID541572.pdf |
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