Development of microsatellite markers in papaya: Isolation, characterization and cross amplification in mountain papayas
Microsatellite-containing sequences were isolated from an enriched genomic library of Carica papaya L. A total of 507 clones were evaluated with radioactive probes containing the motifs (GA)15 and (GT)15 and 147 positive clones were isolated and sequenced. The OLIGO 4.06 software was used to define 76 primer pairs. These primers were evaluated on three genotypes of C papaya and seven species of the genus Vasconcellea. Overall, 69 primer pairs worked in C papaya and 33 in Vasconcellea. The 35 pairs producing the best banding profiles were characterized on a sample of 29 individuals of C. papaya from the Caribbean region and 11 individuals of Vasconcellea from the Andes of Colombia and Ecuador. Twenty-six primer pairs gave polymorphism, of which 24 within C. papaya and only four within the seven Vasconcellea species, confirming the divergence between both genera. The number of alleles was 99, with a mean of 4 and an expected heterozygosity (He) of 0.56 per locus in C papaya. and 18, i.e. 4.5 per locus, in Vasconcellea, in the homozygous state. Microsatellite markers revealed more polymorphism in papaya than AFLP or RAPD.
Main Authors: | , , , , , |
---|---|
Format: | article biblioteca |
Language: | eng |
Subjects: | F30 - Génétique et amélioration des plantes, |
Online Access: | http://agritrop.cirad.fr/534644/ http://agritrop.cirad.fr/534644/1/document_534644.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-534644 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5346442022-12-30T18:30:21Z http://agritrop.cirad.fr/534644/ http://agritrop.cirad.fr/534644/ Development of microsatellite markers in papaya: Isolation, characterization and cross amplification in mountain papayas. Ocampo Perez John Albeiro, Dambier Dominique, Ollitrault Patrick, Coppens D'Eeckenbrugge Géo, Brottier Philippe, Risterucci Ange-Marie. 2005. Proceedings of the Interamerican Society for Tropical Horticulture, 48 : 90-93. Annual Meeting of the Interamerican Society for Tropical Horticulture. 50, Limon, Costa Rica, 7 Octobre 2004/11 Octobre 2004. Researchers Development of microsatellite markers in papaya: Isolation, characterization and cross amplification in mountain papayas Ocampo Perez, John Albeiro Dambier, Dominique Ollitrault, Patrick Coppens D'Eeckenbrugge, Géo Brottier, Philippe Risterucci, Ange-Marie eng 2005 Proceedings of the Interamerican Society for Tropical Horticulture F30 - Génétique et amélioration des plantes Microsatellite-containing sequences were isolated from an enriched genomic library of Carica papaya L. A total of 507 clones were evaluated with radioactive probes containing the motifs (GA)15 and (GT)15 and 147 positive clones were isolated and sequenced. The OLIGO 4.06 software was used to define 76 primer pairs. These primers were evaluated on three genotypes of C papaya and seven species of the genus Vasconcellea. Overall, 69 primer pairs worked in C papaya and 33 in Vasconcellea. The 35 pairs producing the best banding profiles were characterized on a sample of 29 individuals of C. papaya from the Caribbean region and 11 individuals of Vasconcellea from the Andes of Colombia and Ecuador. Twenty-six primer pairs gave polymorphism, of which 24 within C. papaya and only four within the seven Vasconcellea species, confirming the divergence between both genera. The number of alleles was 99, with a mean of 4 and an expected heterozygosity (He) of 0.56 per locus in C papaya. and 18, i.e. 4.5 per locus, in Vasconcellea, in the homozygous state. Microsatellite markers revealed more polymorphism in papaya than AFLP or RAPD. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/534644/1/document_534644.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
F30 - Génétique et amélioration des plantes F30 - Génétique et amélioration des plantes |
spellingShingle |
F30 - Génétique et amélioration des plantes F30 - Génétique et amélioration des plantes Ocampo Perez, John Albeiro Dambier, Dominique Ollitrault, Patrick Coppens D'Eeckenbrugge, Géo Brottier, Philippe Risterucci, Ange-Marie Development of microsatellite markers in papaya: Isolation, characterization and cross amplification in mountain papayas |
description |
Microsatellite-containing sequences were isolated from an enriched genomic library of Carica papaya L. A total of 507 clones were evaluated with radioactive probes containing the motifs (GA)15 and (GT)15 and 147 positive clones were isolated and sequenced. The OLIGO 4.06 software was used to define 76 primer pairs. These primers were evaluated on three genotypes of C papaya and seven species of the genus Vasconcellea. Overall, 69 primer pairs worked in C papaya and 33 in Vasconcellea. The 35 pairs producing the best banding profiles were characterized on a sample of 29 individuals of C. papaya from the Caribbean region and 11 individuals of Vasconcellea from the Andes of Colombia and Ecuador. Twenty-six primer pairs gave polymorphism, of which 24 within C. papaya and only four within the seven Vasconcellea species, confirming the divergence between both genera. The number of alleles was 99, with a mean of 4 and an expected heterozygosity (He) of 0.56 per locus in C papaya. and 18, i.e. 4.5 per locus, in Vasconcellea, in the homozygous state. Microsatellite markers revealed more polymorphism in papaya than AFLP or RAPD. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes |
author |
Ocampo Perez, John Albeiro Dambier, Dominique Ollitrault, Patrick Coppens D'Eeckenbrugge, Géo Brottier, Philippe Risterucci, Ange-Marie |
author_facet |
Ocampo Perez, John Albeiro Dambier, Dominique Ollitrault, Patrick Coppens D'Eeckenbrugge, Géo Brottier, Philippe Risterucci, Ange-Marie |
author_sort |
Ocampo Perez, John Albeiro |
title |
Development of microsatellite markers in papaya: Isolation, characterization and cross amplification in mountain papayas |
title_short |
Development of microsatellite markers in papaya: Isolation, characterization and cross amplification in mountain papayas |
title_full |
Development of microsatellite markers in papaya: Isolation, characterization and cross amplification in mountain papayas |
title_fullStr |
Development of microsatellite markers in papaya: Isolation, characterization and cross amplification in mountain papayas |
title_full_unstemmed |
Development of microsatellite markers in papaya: Isolation, characterization and cross amplification in mountain papayas |
title_sort |
development of microsatellite markers in papaya: isolation, characterization and cross amplification in mountain papayas |
url |
http://agritrop.cirad.fr/534644/ http://agritrop.cirad.fr/534644/1/document_534644.pdf |
work_keys_str_mv |
AT ocampoperezjohnalbeiro developmentofmicrosatellitemarkersinpapayaisolationcharacterizationandcrossamplificationinmountainpapayas AT dambierdominique developmentofmicrosatellitemarkersinpapayaisolationcharacterizationandcrossamplificationinmountainpapayas AT ollitraultpatrick developmentofmicrosatellitemarkersinpapayaisolationcharacterizationandcrossamplificationinmountainpapayas AT coppensdeeckenbruggegeo developmentofmicrosatellitemarkersinpapayaisolationcharacterizationandcrossamplificationinmountainpapayas AT brottierphilippe developmentofmicrosatellitemarkersinpapayaisolationcharacterizationandcrossamplificationinmountainpapayas AT risterucciangemarie developmentofmicrosatellitemarkersinpapayaisolationcharacterizationandcrossamplificationinmountainpapayas |
_version_ |
1758021060743135232 |