Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: a collaborative trial
Diagnostic differentiation between the ruminant pathogens Mycoplasma agalactiae and Mycoplasma bovis is known to be problematic when only conventional serological and biochemical tests are used. The main reason for this is that both agents share a considerable number of related proteins and common epitopes. DNA-based detection methods offer advantages in terms of specificity and sensitivity. However, there is an urgent need to compare currently used PCR assays because they target different genomic regions and, therefore, may perform differently. In the present work, five laboratories, which use PCR routinely, evaluated the specificity of four different PCR systems for M. agalactiae and three systems for M. bovis on a total of 41 strains of the two Mycoplasma species including six previously unidentified strains. As the vast majority of PCR examinations (97.1% of all tests) correctly identified the strains the specificity of all seven detection systems appears to be high. In four cases, incorrect identification by conventional diagnostic methods was rectified by PCR. Isolates from non-typical hosts, i.e. three M. bovis strains from small ruminants and two M. agalactiae strains from cattle, were characterised by sequencing the 16S and part of the 23S ribosomal RNA genes.
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Subjects: | L50 - Physiologie et biochimie animales, Mycoplasma bovis, Mycoplasma, biochimie, identification, Mycoplasma agalactiae, http://aims.fao.org/aos/agrovoc/c_27241, http://aims.fao.org/aos/agrovoc/c_5020, http://aims.fao.org/aos/agrovoc/c_910, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_37782, |
Online Access: | http://agritrop.cirad.fr/525758/ http://agritrop.cirad.fr/525758/1/525758.pdf |
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dig-cirad-fr-5257582024-01-28T13:30:11Z http://agritrop.cirad.fr/525758/ http://agritrop.cirad.fr/525758/ Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: a collaborative trial. Bashiruddin John B., Frey Joachim, Heldtander Königsson Malin, Johansson Karl-Erik, Hotzel Helmut, Diller Roland, De Santis Paola, Botelho Ana, Ayling Roger D., Nicholas Robin A.J., Thiaucourt François, Sachse Konrad. 2005. Veterinary Journal, 169 (2) : 268-275.https://doi.org/10.1016/j.tvjl.2004.01.018 <https://doi.org/10.1016/j.tvjl.2004.01.018> Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: a collaborative trial Bashiruddin, John B. Frey, Joachim Heldtander Königsson, Malin Johansson, Karl-Erik Hotzel, Helmut Diller, Roland De Santis, Paola Botelho, Ana Ayling, Roger D. Nicholas, Robin A.J. Thiaucourt, François Sachse, Konrad eng 2005 Veterinary Journal L50 - Physiologie et biochimie animales Mycoplasma bovis Mycoplasma biochimie identification Mycoplasma agalactiae http://aims.fao.org/aos/agrovoc/c_27241 http://aims.fao.org/aos/agrovoc/c_5020 http://aims.fao.org/aos/agrovoc/c_910 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_37782 Diagnostic differentiation between the ruminant pathogens Mycoplasma agalactiae and Mycoplasma bovis is known to be problematic when only conventional serological and biochemical tests are used. The main reason for this is that both agents share a considerable number of related proteins and common epitopes. DNA-based detection methods offer advantages in terms of specificity and sensitivity. However, there is an urgent need to compare currently used PCR assays because they target different genomic regions and, therefore, may perform differently. In the present work, five laboratories, which use PCR routinely, evaluated the specificity of four different PCR systems for M. agalactiae and three systems for M. bovis on a total of 41 strains of the two Mycoplasma species including six previously unidentified strains. As the vast majority of PCR examinations (97.1% of all tests) correctly identified the strains the specificity of all seven detection systems appears to be high. In four cases, incorrect identification by conventional diagnostic methods was rectified by PCR. Isolates from non-typical hosts, i.e. three M. bovis strains from small ruminants and two M. agalactiae strains from cattle, were characterised by sequencing the 16S and part of the 23S ribosomal RNA genes. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/525758/1/525758.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.tvjl.2004.01.018 10.1016/j.tvjl.2004.01.018 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=186120 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tvjl.2004.01.018 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.tvjl.2004.01.018 |
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L50 - Physiologie et biochimie animales Mycoplasma bovis Mycoplasma biochimie identification Mycoplasma agalactiae http://aims.fao.org/aos/agrovoc/c_27241 http://aims.fao.org/aos/agrovoc/c_5020 http://aims.fao.org/aos/agrovoc/c_910 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_37782 L50 - Physiologie et biochimie animales Mycoplasma bovis Mycoplasma biochimie identification Mycoplasma agalactiae http://aims.fao.org/aos/agrovoc/c_27241 http://aims.fao.org/aos/agrovoc/c_5020 http://aims.fao.org/aos/agrovoc/c_910 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_37782 |
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L50 - Physiologie et biochimie animales Mycoplasma bovis Mycoplasma biochimie identification Mycoplasma agalactiae http://aims.fao.org/aos/agrovoc/c_27241 http://aims.fao.org/aos/agrovoc/c_5020 http://aims.fao.org/aos/agrovoc/c_910 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_37782 L50 - Physiologie et biochimie animales Mycoplasma bovis Mycoplasma biochimie identification Mycoplasma agalactiae http://aims.fao.org/aos/agrovoc/c_27241 http://aims.fao.org/aos/agrovoc/c_5020 http://aims.fao.org/aos/agrovoc/c_910 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_37782 Bashiruddin, John B. Frey, Joachim Heldtander Königsson, Malin Johansson, Karl-Erik Hotzel, Helmut Diller, Roland De Santis, Paola Botelho, Ana Ayling, Roger D. Nicholas, Robin A.J. Thiaucourt, François Sachse, Konrad Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: a collaborative trial |
description |
Diagnostic differentiation between the ruminant pathogens Mycoplasma agalactiae and Mycoplasma bovis is known to be problematic when only conventional serological and biochemical tests are used. The main reason for this is that both agents share a considerable number of related proteins and common epitopes. DNA-based detection methods offer advantages in terms of specificity and sensitivity. However, there is an urgent need to compare currently used PCR assays because they target different genomic regions and, therefore, may perform differently. In the present work, five laboratories, which use PCR routinely, evaluated the specificity of four different PCR systems for M. agalactiae and three systems for M. bovis on a total of 41 strains of the two Mycoplasma species including six previously unidentified strains. As the vast majority of PCR examinations (97.1% of all tests) correctly identified the strains the specificity of all seven detection systems appears to be high. In four cases, incorrect identification by conventional diagnostic methods was rectified by PCR. Isolates from non-typical hosts, i.e. three M. bovis strains from small ruminants and two M. agalactiae strains from cattle, were characterised by sequencing the 16S and part of the 23S ribosomal RNA genes. |
format |
article |
topic_facet |
L50 - Physiologie et biochimie animales Mycoplasma bovis Mycoplasma biochimie identification Mycoplasma agalactiae http://aims.fao.org/aos/agrovoc/c_27241 http://aims.fao.org/aos/agrovoc/c_5020 http://aims.fao.org/aos/agrovoc/c_910 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_37782 |
author |
Bashiruddin, John B. Frey, Joachim Heldtander Königsson, Malin Johansson, Karl-Erik Hotzel, Helmut Diller, Roland De Santis, Paola Botelho, Ana Ayling, Roger D. Nicholas, Robin A.J. Thiaucourt, François Sachse, Konrad |
author_facet |
Bashiruddin, John B. Frey, Joachim Heldtander Königsson, Malin Johansson, Karl-Erik Hotzel, Helmut Diller, Roland De Santis, Paola Botelho, Ana Ayling, Roger D. Nicholas, Robin A.J. Thiaucourt, François Sachse, Konrad |
author_sort |
Bashiruddin, John B. |
title |
Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: a collaborative trial |
title_short |
Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: a collaborative trial |
title_full |
Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: a collaborative trial |
title_fullStr |
Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: a collaborative trial |
title_full_unstemmed |
Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: a collaborative trial |
title_sort |
evaluation of pcr systems for the identification and differentiation of mycoplasma agalactiae and mycoplasma bovis: a collaborative trial |
url |
http://agritrop.cirad.fr/525758/ http://agritrop.cirad.fr/525758/1/525758.pdf |
work_keys_str_mv |
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