Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs
A full-length cDNA (CaSUI1) was isolated from a Coffea arabica cDNA library from beans during maturation. Its putative translational product is highly homologous to the SUI1 protein of Saccharomyces cerevisiae which functions in concert with eIF2 and the initiator tRNA-Methionin in directing the ribosome to the proper start site of translation. The corresponding gene from coffee was also cloned and sequenced. Its organization is very similar to what observed for the same gene from rice (Oryza sativa) particularly regarding the position and length of its introns. The expression of CaSUI1 gene was also checked and showed that it was highly expressed in all coffee tissues analyzed, therefore confirming the essential role of the SUI1 protein in cell housekeeping functions.
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Subjects: | F30 - Génétique et amélioration des plantes, Coffea arabica, Clonage moléculaire, expression des gènes, facteur de transcription, protéine, Saccharomyces cerevisiae, http://aims.fao.org/aos/agrovoc/c_1721, http://aims.fao.org/aos/agrovoc/c_27503, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_35345, http://aims.fao.org/aos/agrovoc/c_6259, http://aims.fao.org/aos/agrovoc/c_6724, |
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dig-cirad-fr-5136912024-01-28T11:25:54Z http://agritrop.cirad.fr/513691/ http://agritrop.cirad.fr/513691/ Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs. Gaborit Charlotte, Caillet Victoria, Deshayes Alain, Marraccini Pierre. 2003. Brazilian Journal of Plant Physiology, 15 (1) : 55-58. Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs Gaborit, Charlotte Caillet, Victoria Deshayes, Alain Marraccini, Pierre eng 2003 Brazilian Journal of Plant Physiology F30 - Génétique et amélioration des plantes Coffea arabica Clonage moléculaire expression des gènes facteur de transcription protéine Saccharomyces cerevisiae http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_27503 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35345 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_6724 A full-length cDNA (CaSUI1) was isolated from a Coffea arabica cDNA library from beans during maturation. Its putative translational product is highly homologous to the SUI1 protein of Saccharomyces cerevisiae which functions in concert with eIF2 and the initiator tRNA-Methionin in directing the ribosome to the proper start site of translation. The corresponding gene from coffee was also cloned and sequenced. Its organization is very similar to what observed for the same gene from rice (Oryza sativa) particularly regarding the position and length of its introns. The expression of CaSUI1 gene was also checked and showed that it was highly expressed in all coffee tissues analyzed, therefore confirming the essential role of the SUI1 protein in cell housekeeping functions. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/513691/1/document_513691.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html |
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F30 - Génétique et amélioration des plantes Coffea arabica Clonage moléculaire expression des gènes facteur de transcription protéine Saccharomyces cerevisiae http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_27503 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35345 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_6724 F30 - Génétique et amélioration des plantes Coffea arabica Clonage moléculaire expression des gènes facteur de transcription protéine Saccharomyces cerevisiae http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_27503 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35345 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_6724 |
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F30 - Génétique et amélioration des plantes Coffea arabica Clonage moléculaire expression des gènes facteur de transcription protéine Saccharomyces cerevisiae http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_27503 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35345 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_6724 F30 - Génétique et amélioration des plantes Coffea arabica Clonage moléculaire expression des gènes facteur de transcription protéine Saccharomyces cerevisiae http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_27503 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35345 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_6724 Gaborit, Charlotte Caillet, Victoria Deshayes, Alain Marraccini, Pierre Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs |
description |
A full-length cDNA (CaSUI1) was isolated from a Coffea arabica cDNA library from beans during maturation. Its putative translational product is highly homologous to the SUI1 protein of Saccharomyces cerevisiae which functions in concert with eIF2 and the initiator tRNA-Methionin in directing the ribosome to the proper start site of translation. The corresponding gene from coffee was also cloned and sequenced. Its organization is very similar to what observed for the same gene from rice (Oryza sativa) particularly regarding the position and length of its introns. The expression of CaSUI1 gene was also checked and showed that it was highly expressed in all coffee tissues analyzed, therefore confirming the essential role of the SUI1 protein in cell housekeeping functions. |
format |
article |
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F30 - Génétique et amélioration des plantes Coffea arabica Clonage moléculaire expression des gènes facteur de transcription protéine Saccharomyces cerevisiae http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_27503 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35345 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_6724 |
author |
Gaborit, Charlotte Caillet, Victoria Deshayes, Alain Marraccini, Pierre |
author_facet |
Gaborit, Charlotte Caillet, Victoria Deshayes, Alain Marraccini, Pierre |
author_sort |
Gaborit, Charlotte |
title |
Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs |
title_short |
Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs |
title_full |
Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs |
title_fullStr |
Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs |
title_full_unstemmed |
Molecular cloning of a full-length cDNA and gene from Coffea arabica encoding a protein homologous to the yeast translation initiation factor SUI1: expression analysis in plant organs |
title_sort |
molecular cloning of a full-length cdna and gene from coffea arabica encoding a protein homologous to the yeast translation initiation factor sui1: expression analysis in plant organs |
url |
http://agritrop.cirad.fr/513691/ http://agritrop.cirad.fr/513691/1/document_513691.pdf |
work_keys_str_mv |
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