Influence of the 20-kDa protein from Bacillus thuringensis sp. israelensis on the rate of production of truncated crylC proteins
The potential role of a molecular chaperone on the rate of production of extensively altered "Bacillus thuringiensis# Cry I C proteins was investigated. Analysis of the proteins produced by the recombinant #B. thuringiensis# strains showed that the truncated proteins were produced at a low rate. Expression of the 20-kDa protein gene from #B. thuringiensis# sp. #israelensis# in tandem with the truncated-crylC genes led to the production of a greater amount of proteins. The formation of inclusion bodies, however, did not occur even when the 20-kDa protein gene was expressed.
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Main Authors: | Rang, Cécile, Bes, Martine, Lullien-Pellerin, Valérie, Wu, D., Federici, B.A., Frutos, Roger |
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Format: | article biblioteca |
Language: | eng |
Subjects: | H10 - Ravageurs des plantes, Bacillus thuringiensis, protéine microbienne, propriété biologique, lutte anti-insecte, http://aims.fao.org/aos/agrovoc/c_761, http://aims.fao.org/aos/agrovoc/c_12537, http://aims.fao.org/aos/agrovoc/c_1501, http://aims.fao.org/aos/agrovoc/c_3885, |
Online Access: | http://agritrop.cirad.fr/391530/ http://agritrop.cirad.fr/391530/1/391530.pdf |
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