Production of high-frequency embryogenic calli from integuments of immature seeds of Carica papaya L.
Tissue culture conditions that enable production in #Carica papaya# L. Of high frequency embryogenic calli on the internal integuments of immature seeds are defined: the explants have to be removed three or four months after anthesis and cultured on three monthly successive media: M1-1 (2,4-D 0.09 microM + KIN 1 microM), M3 (M2 + ABA 0.5 MicroM). A histocytological study during culture proved that friable calli capable of producing embryos were of maternal origin. This procedure thus permits the cloning of adult plants.
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| Main Authors: | , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | F02 - Multiplication végétative des plantes, Carica papaya, graine, testa, cal, embryon somatique, culture d'embryon, http://aims.fao.org/aos/agrovoc/c_1323, http://aims.fao.org/aos/agrovoc/c_6946, http://aims.fao.org/aos/agrovoc/c_7681, http://aims.fao.org/aos/agrovoc/c_24576, http://aims.fao.org/aos/agrovoc/c_27607, http://aims.fao.org/aos/agrovoc/c_2544, |
| Online Access: | http://agritrop.cirad.fr/389884/ |
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