Assessment of RAPD markers to detect genetic change in protoplast-derived rice plants
Fourteen different primers were used to amplify DNA from selfed progenies of 117 diploid plants regenerated from haploid cell suspension protoplast of rice (cv. Miara). This survey, which permitted to generate 69 different major fragments in control Miara plants as well as in protoclone DNA, did not reveal genetic change among protoclones compared to the donor variety except independent amplification of an additional fragment in nearly half of the lines by two 10-mers. This polymorphism in the protoclonal population was attributed to natural intravarietal variation among the donor plants which have been collected to establish the microscope-callus suspension culture. However, polymorphism could not be detected in calluses grown from cell clumps collected in the 21-month old cell suspension. This apparent lack of tissue culture-induced polymorphism at the molecular level contrasts with the results of a previous field evaluation of the progenies which exhibited both wide range and high frequency morphological changes. These observations highlight the absence of apparent relationship between morphological variation and molecular changes. The interest of RAPDs as a convenient method to test genetic integrity of the regenerants, that would preclude the need for further field evaluation, is discussed.
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Subjects: | F30 - Génétique et amélioration des plantes, Oryza, marqueur génétique, RAPD, adn, fusion de protoplastes, http://aims.fao.org/aos/agrovoc/c_5435, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_36341, http://aims.fao.org/aos/agrovoc/c_2347, http://aims.fao.org/aos/agrovoc/c_24888, |
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dig-cirad-fr-3897972024-01-27T23:04:38Z http://agritrop.cirad.fr/389797/ http://agritrop.cirad.fr/389797/ Assessment of RAPD markers to detect genetic change in protoplast-derived rice plants. Mezencev N., Ghesquière Alain, Marney P., Combes Marie-Christine, Guiderdoni Emmanuel. 1997. Journal of Genetics and Breeding, 51 (2) : 97-102. Assessment of RAPD markers to detect genetic change in protoplast-derived rice plants Mezencev, N. Ghesquière, Alain Marney, P. Combes, Marie-Christine Guiderdoni, Emmanuel eng 1997 Journal of Genetics and Breeding F30 - Génétique et amélioration des plantes Oryza marqueur génétique RAPD adn fusion de protoplastes http://aims.fao.org/aos/agrovoc/c_5435 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36341 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_24888 Fourteen different primers were used to amplify DNA from selfed progenies of 117 diploid plants regenerated from haploid cell suspension protoplast of rice (cv. Miara). This survey, which permitted to generate 69 different major fragments in control Miara plants as well as in protoclone DNA, did not reveal genetic change among protoclones compared to the donor variety except independent amplification of an additional fragment in nearly half of the lines by two 10-mers. This polymorphism in the protoclonal population was attributed to natural intravarietal variation among the donor plants which have been collected to establish the microscope-callus suspension culture. However, polymorphism could not be detected in calluses grown from cell clumps collected in the 21-month old cell suspension. This apparent lack of tissue culture-induced polymorphism at the molecular level contrasts with the results of a previous field evaluation of the progenies which exhibited both wide range and high frequency morphological changes. These observations highlight the absence of apparent relationship between morphological variation and molecular changes. The interest of RAPDs as a convenient method to test genetic integrity of the regenerants, that would preclude the need for further field evaluation, is discussed. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/closedAccess http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=89505 |
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F30 - Génétique et amélioration des plantes Oryza marqueur génétique RAPD adn fusion de protoplastes http://aims.fao.org/aos/agrovoc/c_5435 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36341 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_24888 F30 - Génétique et amélioration des plantes Oryza marqueur génétique RAPD adn fusion de protoplastes http://aims.fao.org/aos/agrovoc/c_5435 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36341 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_24888 |
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F30 - Génétique et amélioration des plantes Oryza marqueur génétique RAPD adn fusion de protoplastes http://aims.fao.org/aos/agrovoc/c_5435 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36341 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_24888 F30 - Génétique et amélioration des plantes Oryza marqueur génétique RAPD adn fusion de protoplastes http://aims.fao.org/aos/agrovoc/c_5435 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36341 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_24888 Mezencev, N. Ghesquière, Alain Marney, P. Combes, Marie-Christine Guiderdoni, Emmanuel Assessment of RAPD markers to detect genetic change in protoplast-derived rice plants |
description |
Fourteen different primers were used to amplify DNA from selfed progenies of 117 diploid plants regenerated from haploid cell suspension protoplast of rice (cv. Miara). This survey, which permitted to generate 69 different major fragments in control Miara plants as well as in protoclone DNA, did not reveal genetic change among protoclones compared to the donor variety except independent amplification of an additional fragment in nearly half of the lines by two 10-mers. This polymorphism in the protoclonal population was attributed to natural intravarietal variation among the donor plants which have been collected to establish the microscope-callus suspension culture. However, polymorphism could not be detected in calluses grown from cell clumps collected in the 21-month old cell suspension. This apparent lack of tissue culture-induced polymorphism at the molecular level contrasts with the results of a previous field evaluation of the progenies which exhibited both wide range and high frequency morphological changes. These observations highlight the absence of apparent relationship between morphological variation and molecular changes. The interest of RAPDs as a convenient method to test genetic integrity of the regenerants, that would preclude the need for further field evaluation, is discussed. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes Oryza marqueur génétique RAPD adn fusion de protoplastes http://aims.fao.org/aos/agrovoc/c_5435 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36341 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_24888 |
author |
Mezencev, N. Ghesquière, Alain Marney, P. Combes, Marie-Christine Guiderdoni, Emmanuel |
author_facet |
Mezencev, N. Ghesquière, Alain Marney, P. Combes, Marie-Christine Guiderdoni, Emmanuel |
author_sort |
Mezencev, N. |
title |
Assessment of RAPD markers to detect genetic change in protoplast-derived rice plants |
title_short |
Assessment of RAPD markers to detect genetic change in protoplast-derived rice plants |
title_full |
Assessment of RAPD markers to detect genetic change in protoplast-derived rice plants |
title_fullStr |
Assessment of RAPD markers to detect genetic change in protoplast-derived rice plants |
title_full_unstemmed |
Assessment of RAPD markers to detect genetic change in protoplast-derived rice plants |
title_sort |
assessment of rapd markers to detect genetic change in protoplast-derived rice plants |
url |
http://agritrop.cirad.fr/389797/ |
work_keys_str_mv |
AT mezencevn assessmentofrapdmarkerstodetectgeneticchangeinprotoplastderivedriceplants AT ghesquierealain assessmentofrapdmarkerstodetectgeneticchangeinprotoplastderivedriceplants AT marneyp assessmentofrapdmarkerstodetectgeneticchangeinprotoplastderivedriceplants AT combesmariechristine assessmentofrapdmarkerstodetectgeneticchangeinprotoplastderivedriceplants AT guiderdoniemmanuel assessmentofrapdmarkerstodetectgeneticchangeinprotoplastderivedriceplants |
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1792491236084416512 |