Determination of ethyl sulfate in sewage water using ion-pair UHPLC-MS/MS

Determination of ethyl sulfate in sewage water using ion-pair UHPLC-MS/MS

Póster presentado en el congreso 10th LC/MS/MS Workshop On Environmental Applications And Food Safety, celebrado en Barcelona, España, del 1 al 3 de julio de 2014

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Main Authors: Andrés-Costa, María Jesús, Andreu Pérez, V., Picó, Yolanda
Other Authors: Ministerio de Economía y Competitividad (España)
Format: póster de congreso biblioteca
Published: 2014-07-01
Online Access:http://hdl.handle.net/10261/139624
http://dx.doi.org/10.13039/501100003329
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spelling dig-cide-es-10261-1396242019-10-01T11:06:31Z Determination of ethyl sulfate in sewage water using ion-pair UHPLC-MS/MS Andrés-Costa, María Jesús Andreu Pérez, V. Picó, Yolanda Ministerio de Economía y Competitividad (España) Póster presentado en el congreso 10th LC/MS/MS Workshop On Environmental Applications And Food Safety, celebrado en Barcelona, España, del 1 al 3 de julio de 2014 The alcohol consumption by population could be estimated using the method based on the measurement of specific urinary metabolites in the influent of the Waste Water Treatment Plant (WWTP). Sewage epidemiology has previously been demonstrated far pharmaceuticals and drugs of abuse (Thomas et al., 2012; Zuccato et al. , 2008). The urinary metabolite that was used for this purpose was the Ethyl sulfate (ES) that is excreted in urine as a minor metabolite (0 .011 % on molar basis) after consume alcoholic beverages, being an useful biomarker (Hoiseth et al., 2008). The aim of this study is to develop a reliable and brief method to determine the ES carried out by ion-pair mass spectrometry. The validated method was applied to sewage samples of the influent of 3 WWTP, Pineda 1, Pineda 11 and Quart-Benager, that treats most of the wastewater of Valencia City and its surrounding towns. Sampling campaign was in 2014 from March 4th to 20th. Two approaches were evaluated. The addition of the ion-pair was into the mobile phase or only to the sample. As well, different ion-pair were tested as tetrabuthylamonium clorure, dihexylamine, tributylamine, acetic acid and ammonium formate. Finally, the samples were prepared by adding 50 mM of the ion-pair in raw waters. Chromatographic separation of ES was performed using an Agilent Technologies 1260 UHPLC. The column was Kinetex C 18 (1.7 ¿m, 100 A, 50 x 2.1 O mm) maintained at temperature of 30 ºC and a constant flow rate of 0.2 ml/min. The mobile phase was eluent A (formic acid 0.1 % in water) and eluent B (formic acid 0.1 % in methanol). The gradient elution was 10% B until 5 min. The injected vol u me was 5 ¿L. The UHPLC was coupled to an Agilent Technologies 641 O triple quadrupole mass spectrometer with an electrospray Turbo V ionization source working in negative ionization (NI) mode, 300ºC gas temperature, 11 L min-1 gas flow and 25 psi nebulizer. The determination of ES in the influent of the selected WWTPs shows that this compound was detected in 100% of influents from Pineda 1, Pineda 11 and Quart-Benager. Its consumption was variable during week and weekend, being higher on weekends. This work has been supported by the Spanish Ministry of Economy and Competitiveness trough the project SCARCE-CDS 2009-0065, CGL 2011 -29703-C02-01 and GCL CGL 2011- 29703-C02-02. MJ Andrés Costa also acknowledges to this Ministry the FPI grant to perform her PhD Peer Reviewed 2016-10-31T09:44:45Z 2016-10-31T09:44:45Z 2014-07-01 2016-10-31T09:44:45Z póster de congreso http://purl.org/coar/resource_type/c_6670 10th LC/MS/MS Workshop On Environmental Applications And Food Safety (2014) http://hdl.handle.net/10261/139624 http://dx.doi.org/10.13039/501100003329 Sí none
institution CIDE ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-cide-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del CIDE España
description Póster presentado en el congreso 10th LC/MS/MS Workshop On Environmental Applications And Food Safety, celebrado en Barcelona, España, del 1 al 3 de julio de 2014
author2 Ministerio de Economía y Competitividad (España)
author_facet Ministerio de Economía y Competitividad (España)
Andrés-Costa, María Jesús
Andreu Pérez, V.
Picó, Yolanda
format póster de congreso
author Andrés-Costa, María Jesús
Andreu Pérez, V.
Picó, Yolanda
spellingShingle Andrés-Costa, María Jesús
Andreu Pérez, V.
Picó, Yolanda
Determination of ethyl sulfate in sewage water using ion-pair UHPLC-MS/MS
author_sort Andrés-Costa, María Jesús
title Determination of ethyl sulfate in sewage water using ion-pair UHPLC-MS/MS
title_short Determination of ethyl sulfate in sewage water using ion-pair UHPLC-MS/MS
title_full Determination of ethyl sulfate in sewage water using ion-pair UHPLC-MS/MS
title_fullStr Determination of ethyl sulfate in sewage water using ion-pair UHPLC-MS/MS
title_full_unstemmed Determination of ethyl sulfate in sewage water using ion-pair UHPLC-MS/MS
title_sort determination of ethyl sulfate in sewage water using ion-pair uhplc-ms/ms
publishDate 2014-07-01
url http://hdl.handle.net/10261/139624
http://dx.doi.org/10.13039/501100003329
work_keys_str_mv AT andrescostamariajesus determinationofethylsulfateinsewagewaterusingionpairuhplcmsms
AT andreuperezv determinationofethylsulfateinsewagewaterusingionpairuhplcmsms
AT picoyolanda determinationofethylsulfateinsewagewaterusingionpairuhplcmsms
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