Hidden tenants: Microbiota of the rhizosphere and Phyllosphere of Cordia dodecandra trees in mayan forests and homegardens
During domestication, the selection of cultivated plants often reduces microbiota diversity compared with their wild ancestors. Microbiota in compartments such as the phyllosphere or rhizosphere can promote fruit tree health, growth, and development. Cordia dodecandra is a deciduous tree used by Maya people for its fruit and wood, growing, to date, in remnant forest fragments and homegardens (traditional agroforestry systems) in Yucatán. In this work, we evaluated the microbiota’s alpha and beta diversity per compartment (phyllosphere and rhizosphere) and per population (forest and homegarden) in the Northeast and Southwest Yucatán regions. Eight composite DNA samples (per compartment/population/region combination) were amplified for 16S-RNA (bacteria) and ITS1-2 (fungi) and sequenced by Illumina MiSeq. Bioinformatic analyses were performed with QIIME and phyloseq. For bacteria and fungi, from 107,947 and 128,786 assembled sequences, 618 and 1092 operating taxonomic units (OTUs) were assigned, respectively. The alpha diversity of bacteria and fungi was highly variable among samples and was similar among compartments and populations. A significant species turnover among populations and regions was observed in the rhizosphere. The core microbiota from the phyllosphere was similar among populations and regions. Forests and homegarden populations are reservoirs of the C. dodecandra phyllosphere core microbiome and significant rhizosphere biodiversity. © 2022 by the authors.
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Subjects: | info:eu-repo/classification/Autores/AGROFORESTRY SYSTEMS, info:eu-repo/classification/Autores/16S RRNA GENE SEQUENCING, info:eu-repo/classification/Autores/DOMESTICATION OF FRUIT TREES, info:eu-repo/classification/Autores/MICROBIAL ECOLOGY, info:eu-repo/classification/Autores/MICROBIAL COMMUNITIES, info:eu-repo/classification/Autores/FUNGAL ITS SEQUENCING, info:eu-repo/classification/Autores/MISEQ AMPLICON SEQUENCING, info:eu-repo/classification/cti/2, info:eu-repo/classification/cti/24, info:eu-repo/classification/cti/2403, info:eu-repo/classification/cti/230221, |
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dig-cicy-1003-28102023-10-24T16:24:34Z Hidden tenants: Microbiota of the rhizosphere and Phyllosphere of Cordia dodecandra trees in mayan forests and homegardens CARLA G. MAY MUTUL MIGUEL ANGEL LOPEZ GARRIDO Ingrid Aileen O'Connor Sánchez Yuri Jorge Peña-Ramirez NATALIA YSABEL LABRIN SOTOMAYOR HECTOR ESTRADA-MEDINA Miriam Ferrer 2022 info:eu-repo/semantics/article During domestication, the selection of cultivated plants often reduces microbiota diversity compared with their wild ancestors. Microbiota in compartments such as the phyllosphere or rhizosphere can promote fruit tree health, growth, and development. Cordia dodecandra is a deciduous tree used by Maya people for its fruit and wood, growing, to date, in remnant forest fragments and homegardens (traditional agroforestry systems) in Yucatán. In this work, we evaluated the microbiota’s alpha and beta diversity per compartment (phyllosphere and rhizosphere) and per population (forest and homegarden) in the Northeast and Southwest Yucatán regions. Eight composite DNA samples (per compartment/population/region combination) were amplified for 16S-RNA (bacteria) and ITS1-2 (fungi) and sequenced by Illumina MiSeq. Bioinformatic analyses were performed with QIIME and phyloseq. For bacteria and fungi, from 107,947 and 128,786 assembled sequences, 618 and 1092 operating taxonomic units (OTUs) were assigned, respectively. The alpha diversity of bacteria and fungi was highly variable among samples and was similar among compartments and populations. A significant species turnover among populations and regions was observed in the rhizosphere. The core microbiota from the phyllosphere was similar among populations and regions. Forests and homegarden populations are reservoirs of the C. dodecandra phyllosphere core microbiome and significant rhizosphere biodiversity. © 2022 by the authors. info:eu-repo/classification/Autores/AGROFORESTRY SYSTEMS info:eu-repo/classification/Autores/16S RRNA GENE SEQUENCING info:eu-repo/classification/Autores/DOMESTICATION OF FRUIT TREES info:eu-repo/classification/Autores/MICROBIAL ECOLOGY info:eu-repo/classification/Autores/MICROBIAL COMMUNITIES info:eu-repo/classification/Autores/FUNGAL ITS SEQUENCING info:eu-repo/classification/Autores/MISEQ AMPLICON SEQUENCING info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2403 info:eu-repo/classification/cti/230221 info:eu-repo/classification/cti/230221 Plants, 11(22), 3098, 2022. http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2810 info:eu-repo/semantics/datasetDOI/https://doi.org/10.3390/plants11223098 info:eu-repo/semantics/openAccess eng citation:May-Mutul, C.G.; López-Garrido, M.A.; O’Connor-Sánchez, A.; Peña-Ramírez, Y.J.; Labrín-Sotomayor, N.Y.; Estrada-Medina, H.; Ferrer, M.M. Hidden Tenants: Microbiota of the Rhizosphere and Phyllosphere of Cordia dodecandra Trees in Mayan Forests and Homegardens. Plants 2022, 11, 3098.https://doi.org/ 10.3390/plants11223098 http://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/publishedVersion application/pdf |
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info:eu-repo/classification/Autores/AGROFORESTRY SYSTEMS info:eu-repo/classification/Autores/16S RRNA GENE SEQUENCING info:eu-repo/classification/Autores/DOMESTICATION OF FRUIT TREES info:eu-repo/classification/Autores/MICROBIAL ECOLOGY info:eu-repo/classification/Autores/MICROBIAL COMMUNITIES info:eu-repo/classification/Autores/FUNGAL ITS SEQUENCING info:eu-repo/classification/Autores/MISEQ AMPLICON SEQUENCING info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2403 info:eu-repo/classification/cti/230221 info:eu-repo/classification/cti/230221 info:eu-repo/classification/Autores/AGROFORESTRY SYSTEMS info:eu-repo/classification/Autores/16S RRNA GENE SEQUENCING info:eu-repo/classification/Autores/DOMESTICATION OF FRUIT TREES info:eu-repo/classification/Autores/MICROBIAL ECOLOGY info:eu-repo/classification/Autores/MICROBIAL COMMUNITIES info:eu-repo/classification/Autores/FUNGAL ITS SEQUENCING info:eu-repo/classification/Autores/MISEQ AMPLICON SEQUENCING info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2403 info:eu-repo/classification/cti/230221 info:eu-repo/classification/cti/230221 |
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info:eu-repo/classification/Autores/AGROFORESTRY SYSTEMS info:eu-repo/classification/Autores/16S RRNA GENE SEQUENCING info:eu-repo/classification/Autores/DOMESTICATION OF FRUIT TREES info:eu-repo/classification/Autores/MICROBIAL ECOLOGY info:eu-repo/classification/Autores/MICROBIAL COMMUNITIES info:eu-repo/classification/Autores/FUNGAL ITS SEQUENCING info:eu-repo/classification/Autores/MISEQ AMPLICON SEQUENCING info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2403 info:eu-repo/classification/cti/230221 info:eu-repo/classification/cti/230221 info:eu-repo/classification/Autores/AGROFORESTRY SYSTEMS info:eu-repo/classification/Autores/16S RRNA GENE SEQUENCING info:eu-repo/classification/Autores/DOMESTICATION OF FRUIT TREES info:eu-repo/classification/Autores/MICROBIAL ECOLOGY info:eu-repo/classification/Autores/MICROBIAL COMMUNITIES info:eu-repo/classification/Autores/FUNGAL ITS SEQUENCING info:eu-repo/classification/Autores/MISEQ AMPLICON SEQUENCING info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2403 info:eu-repo/classification/cti/230221 info:eu-repo/classification/cti/230221 CARLA G. MAY MUTUL MIGUEL ANGEL LOPEZ GARRIDO Ingrid Aileen O'Connor Sánchez Yuri Jorge Peña-Ramirez NATALIA YSABEL LABRIN SOTOMAYOR HECTOR ESTRADA-MEDINA Miriam Ferrer Hidden tenants: Microbiota of the rhizosphere and Phyllosphere of Cordia dodecandra trees in mayan forests and homegardens |
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During domestication, the selection of cultivated plants often reduces microbiota diversity compared with their wild ancestors. Microbiota in compartments such as the phyllosphere or rhizosphere can promote fruit tree health, growth, and development. Cordia dodecandra is a deciduous tree used by Maya people for its fruit and wood, growing, to date, in remnant forest fragments and homegardens (traditional agroforestry systems) in Yucatán. In this work, we evaluated the microbiota’s alpha and beta diversity per compartment (phyllosphere and rhizosphere) and per population (forest and homegarden) in the Northeast and Southwest Yucatán regions. Eight composite DNA samples (per compartment/population/region combination) were amplified for 16S-RNA (bacteria) and ITS1-2 (fungi) and sequenced by Illumina MiSeq. Bioinformatic analyses were performed with QIIME and phyloseq. For bacteria and fungi, from 107,947 and 128,786 assembled sequences, 618 and 1092 operating taxonomic units (OTUs) were assigned, respectively. The alpha diversity of bacteria and fungi was highly variable among samples and was similar among compartments and populations. A significant species turnover among populations and regions was observed in the rhizosphere. The core microbiota from the phyllosphere was similar among populations and regions. Forests and homegarden populations are reservoirs of the C. dodecandra phyllosphere core microbiome and significant rhizosphere biodiversity. © 2022 by the authors. |
format |
info:eu-repo/semantics/article |
topic_facet |
info:eu-repo/classification/Autores/AGROFORESTRY SYSTEMS info:eu-repo/classification/Autores/16S RRNA GENE SEQUENCING info:eu-repo/classification/Autores/DOMESTICATION OF FRUIT TREES info:eu-repo/classification/Autores/MICROBIAL ECOLOGY info:eu-repo/classification/Autores/MICROBIAL COMMUNITIES info:eu-repo/classification/Autores/FUNGAL ITS SEQUENCING info:eu-repo/classification/Autores/MISEQ AMPLICON SEQUENCING info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2403 info:eu-repo/classification/cti/230221 info:eu-repo/classification/cti/230221 |
author |
CARLA G. MAY MUTUL MIGUEL ANGEL LOPEZ GARRIDO Ingrid Aileen O'Connor Sánchez Yuri Jorge Peña-Ramirez NATALIA YSABEL LABRIN SOTOMAYOR HECTOR ESTRADA-MEDINA Miriam Ferrer |
author_facet |
CARLA G. MAY MUTUL MIGUEL ANGEL LOPEZ GARRIDO Ingrid Aileen O'Connor Sánchez Yuri Jorge Peña-Ramirez NATALIA YSABEL LABRIN SOTOMAYOR HECTOR ESTRADA-MEDINA Miriam Ferrer |
author_sort |
CARLA G. MAY MUTUL |
title |
Hidden tenants: Microbiota of the rhizosphere and Phyllosphere of Cordia dodecandra trees in mayan forests and homegardens |
title_short |
Hidden tenants: Microbiota of the rhizosphere and Phyllosphere of Cordia dodecandra trees in mayan forests and homegardens |
title_full |
Hidden tenants: Microbiota of the rhizosphere and Phyllosphere of Cordia dodecandra trees in mayan forests and homegardens |
title_fullStr |
Hidden tenants: Microbiota of the rhizosphere and Phyllosphere of Cordia dodecandra trees in mayan forests and homegardens |
title_full_unstemmed |
Hidden tenants: Microbiota of the rhizosphere and Phyllosphere of Cordia dodecandra trees in mayan forests and homegardens |
title_sort |
hidden tenants: microbiota of the rhizosphere and phyllosphere of cordia dodecandra trees in mayan forests and homegardens |
url |
http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2810 |
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