Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.)
Plants respond to drought stress through the ABA dependent and independent pathways, which in turn modulate transcriptional regulatory hubs. Here, we employed Illumina RNA-Seq to analyze a total of 18 cDNA libraries from leaves, sap, and roots of papaya plants under drought stress. Reference and de novo transcriptomic analyses identified 8,549 and 6,089 drought-responsive genes and unigenes, respectively. Core sets of 6 and 34 genes were simultaneously up- or down-regulated, respectively, in all stressed samples. Moreover, GO enrichment analysis revealed that under moderate drought stress, processes related to cell cycle and DNA repair were up-regulated in leaves and sap; while responses to abiotic stress, hormone signaling, sucrose metabolism, and suberin biosynthesis were up-regulated in roots. Under severe drought stress, biological processes related to abiotic stress, hormone signaling, and oxidation-reduction were up-regulated in all tissues. Moreover, similar biological processes were commonly down-regulated in all stressed samples. Furthermore, co-expression network analysis revealed three and eight transcriptionally regulated modules in leaves and roots, respectively. Seventeen stress-related TFs were identified, potentially serving as main regulatory hubs in leaves and roots. Our findings provide insight into the molecular responses of papaya plant to drought, which could contribute to the improvement of this important tropical crop.
Main Authors: | SAMUEL DAVID GAMBOA TUZ, ALEJANDRO PEREIRA SANTANA, Jesús Alejandro Zamora Briseño, Enrique Castaño de la Serna, FRANCISCO LEONEL ESPADAS Y GIL, JORGE TONATIUH AYALA SUMUANO, MIGUEL ANGEL KEB LLANES, Lorenzo Felipe Sánchez Teyer, Luis Carlos Rodríguez Zapata |
---|---|
Format: | info:eu-repo/semantics/article biblioteca |
Language: | eng |
Subjects: | info:eu-repo/classification/Autores/PAPAYA, info:eu-repo/classification/Autores/CARICA PAPAYA, info:eu-repo/classification/cti/2, |
Online Access: | http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1562 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Transcriptomic analysis reveals key transcription factors associated to drought tolerance in a wild papaya (Carica papaya) genotype
by: Humberto José Estrella Maldonado, et al. -
Características de calidad de frutos de papaya maradol en la madurez de consumo
by: FELIPE SANTAMARIA BASULTO, et al. -
Validación del uso de marcadores moleculares de sexo y color en híbridos obtenidos de cruzas de Maradol x papaya criolla
by: MARIELA VAZQUEZ CALDERON, et al. -
Control de dos especies de Colletotrichum causantes de antracnosis en frutos de papaya Maradol
by: Felipe Santamaría Basulto, et al. -
Physical characteristics of the leaves and latex of papaya plants infected with the Papaya meleira virus
by: DAISY DE LA CARIDAD PEREZ BRITO