Laccase gene expression and vinasse biodegradation by Trametes hirsuta strain Bm-2

RAUL TAPIA TUSSELL; DAISY DE LA CARIDAD PEREZ BRITO; CLAUDIA GUADALUPE TORRES CALZADA; ALBERTO CORTES VELAZQUEZ; LILIANA MARIA ALZATE GAVIRIA; RUBI DEL ROSARIO CHABLE VILLACIS; SARA ELENA SOLIS PEREIRA

Laccase gene expression and vinasse biodegradation by Trametes hirsuta strain Bm-2

Vinasse is the dark-colored wastewater that is generated by bioethanol distilleries from feedstock molasses. The vinasse that is generated from molasses contains high amounts of pollutants, including phenolic compounds and melanoindin. The goal of this work was to study the expression of laccase genes in the Trametes hirsuta strain Bm-2, isolated in Yucatan, Mexico, in the presence of phenolic compounds, as well as its effectiveness in removing colorants from vinasse. In the presence of all phenolic compounds tested (guaiacol, ferulic acid, and vanillic acid), increased levels of laccase-encoding mRNA were observed. Transcript levels in the presence of guaiacol were 40 times higher than those in the control. The lcc1 and lcc2 genes of T. hirsuta were differentially expressed; guaiacol and vanillin induced the expression of both genes, whereas ferulic acid only induced the expression of lcc2. The discoloration of vinasse was concomitant with the increase in laccase activity. The highest value of enzyme activity (2543.7 U/mL) was obtained in 10% (v/v) vinasse, which corresponded to a 69.2% increase in discoloration. This study demonstrates the potential of the Bm-2 strain of T. hirsuta for the biodegradation of vinasse.

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Main Authors:	RAUL TAPIA TUSSELL, DAISY DE LA CARIDAD PEREZ BRITO, CLAUDIA GUADALUPE TORRES CALZADA, ALBERTO CORTES VELAZQUEZ, LILIANA MARIA ALZATE GAVIRIA, RUBI DEL ROSARIO CHABLE VILLACIS, SARA ELENA SOLIS PEREIRA
Format:	info:eu-repo/semantics/article biblioteca
Language:	eng
Subjects:	info:eu-repo/classification/Autores/TRAMETES HIRSUTA, info:eu-repo/classification/Autores/LACASSES, info:eu-repo/classification/Autores/GENE EXPRESSION, info:eu-repo/classification/Autores/BIODEGRADATION, info:eu-repo/classification/Autores/VINASSE, info:eu-repo/classification/cti/2,
Online Access:	http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1096
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spelling	dig-cicy-1003-10962018-06-26T16:03:33Z Laccase gene expression and vinasse biodegradation by Trametes hirsuta strain Bm-2 RAUL TAPIA TUSSELL DAISY DE LA CARIDAD PEREZ BRITO CLAUDIA GUADALUPE TORRES CALZADA ALBERTO CORTES VELAZQUEZ LILIANA MARIA ALZATE GAVIRIA RUBI DEL ROSARIO CHABLE VILLACIS SARA ELENA SOLIS PEREIRA 2015-08-01 info:eu-repo/semantics/article Vinasse is the dark-colored wastewater that is generated by bioethanol distilleries from feedstock molasses. The vinasse that is generated from molasses contains high amounts of pollutants, including phenolic compounds and melanoindin. The goal of this work was to study the expression of laccase genes in the Trametes hirsuta strain Bm-2, isolated in Yucatan, Mexico, in the presence of phenolic compounds, as well as its effectiveness in removing colorants from vinasse. In the presence of all phenolic compounds tested (guaiacol, ferulic acid, and vanillic acid), increased levels of laccase-encoding mRNA were observed. Transcript levels in the presence of guaiacol were 40 times higher than those in the control. The lcc1 and lcc2 genes of T. hirsuta were differentially expressed; guaiacol and vanillin induced the expression of both genes, whereas ferulic acid only induced the expression of lcc2. The discoloration of vinasse was concomitant with the increase in laccase activity. The highest value of enzyme activity (2543.7 U/mL) was obtained in 10% (v/v) vinasse, which corresponded to a 69.2% increase in discoloration. This study demonstrates the potential of the Bm-2 strain of T. hirsuta for the biodegradation of vinasse. info:eu-repo/classification/Autores/TRAMETES HIRSUTA info:eu-repo/classification/Autores/LACASSES info:eu-repo/classification/Autores/GENE EXPRESSION info:eu-repo/classification/Autores/BIODEGRADATION info:eu-repo/classification/Autores/VINASSE info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 Molecules. 20(8), 15147-15157, 2015 http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1096 info:eu-repo/semantics/datasetDOI/doi:10.3390/molecules200815147 info:eu-repo/semantics/openAccess eng citation:Tapia-Tussell, R., Pérez-Brito, D., Torres-Calzada, C., Cortés-Velázquez, A., Alzate-Gaviria, L., Chablé-Villacís, R., & Solís-Pereira, S. (2015). Laccase Gene Expression and Vinasse Biodegradation by Trametes hirsuta Strain Bm-2. Molecules, 20(8), 15147-15157. http://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/publishedVersion application/pdf
institution	CICY
collection	DSpace
country	México
countrycode	MX
component	Bibliográfico
access	En linea
databasecode	dig-cicy
tag	biblioteca
region	America del Norte
libraryname	Biblioteca del CICY
language	eng
topic	info:eu-repo/classification/Autores/TRAMETES HIRSUTA info:eu-repo/classification/Autores/LACASSES info:eu-repo/classification/Autores/GENE EXPRESSION info:eu-repo/classification/Autores/BIODEGRADATION info:eu-repo/classification/Autores/VINASSE info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 info:eu-repo/classification/Autores/TRAMETES HIRSUTA info:eu-repo/classification/Autores/LACASSES info:eu-repo/classification/Autores/GENE EXPRESSION info:eu-repo/classification/Autores/BIODEGRADATION info:eu-repo/classification/Autores/VINASSE info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2
spellingShingle	info:eu-repo/classification/Autores/TRAMETES HIRSUTA info:eu-repo/classification/Autores/LACASSES info:eu-repo/classification/Autores/GENE EXPRESSION info:eu-repo/classification/Autores/BIODEGRADATION info:eu-repo/classification/Autores/VINASSE info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 info:eu-repo/classification/Autores/TRAMETES HIRSUTA info:eu-repo/classification/Autores/LACASSES info:eu-repo/classification/Autores/GENE EXPRESSION info:eu-repo/classification/Autores/BIODEGRADATION info:eu-repo/classification/Autores/VINASSE info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 RAUL TAPIA TUSSELL DAISY DE LA CARIDAD PEREZ BRITO CLAUDIA GUADALUPE TORRES CALZADA ALBERTO CORTES VELAZQUEZ LILIANA MARIA ALZATE GAVIRIA RUBI DEL ROSARIO CHABLE VILLACIS SARA ELENA SOLIS PEREIRA Laccase gene expression and vinasse biodegradation by Trametes hirsuta strain Bm-2
description	Vinasse is the dark-colored wastewater that is generated by bioethanol distilleries from feedstock molasses. The vinasse that is generated from molasses contains high amounts of pollutants, including phenolic compounds and melanoindin. The goal of this work was to study the expression of laccase genes in the Trametes hirsuta strain Bm-2, isolated in Yucatan, Mexico, in the presence of phenolic compounds, as well as its effectiveness in removing colorants from vinasse. In the presence of all phenolic compounds tested (guaiacol, ferulic acid, and vanillic acid), increased levels of laccase-encoding mRNA were observed. Transcript levels in the presence of guaiacol were 40 times higher than those in the control. The lcc1 and lcc2 genes of T. hirsuta were differentially expressed; guaiacol and vanillin induced the expression of both genes, whereas ferulic acid only induced the expression of lcc2. The discoloration of vinasse was concomitant with the increase in laccase activity. The highest value of enzyme activity (2543.7 U/mL) was obtained in 10% (v/v) vinasse, which corresponded to a 69.2% increase in discoloration. This study demonstrates the potential of the Bm-2 strain of T. hirsuta for the biodegradation of vinasse.
format	info:eu-repo/semantics/article
topic_facet	info:eu-repo/classification/Autores/TRAMETES HIRSUTA info:eu-repo/classification/Autores/LACASSES info:eu-repo/classification/Autores/GENE EXPRESSION info:eu-repo/classification/Autores/BIODEGRADATION info:eu-repo/classification/Autores/VINASSE info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2
author	RAUL TAPIA TUSSELL DAISY DE LA CARIDAD PEREZ BRITO CLAUDIA GUADALUPE TORRES CALZADA ALBERTO CORTES VELAZQUEZ LILIANA MARIA ALZATE GAVIRIA RUBI DEL ROSARIO CHABLE VILLACIS SARA ELENA SOLIS PEREIRA
author_facet	RAUL TAPIA TUSSELL DAISY DE LA CARIDAD PEREZ BRITO CLAUDIA GUADALUPE TORRES CALZADA ALBERTO CORTES VELAZQUEZ LILIANA MARIA ALZATE GAVIRIA RUBI DEL ROSARIO CHABLE VILLACIS SARA ELENA SOLIS PEREIRA
author_sort	RAUL TAPIA TUSSELL
title	Laccase gene expression and vinasse biodegradation by Trametes hirsuta strain Bm-2
title_short	Laccase gene expression and vinasse biodegradation by Trametes hirsuta strain Bm-2
title_full	Laccase gene expression and vinasse biodegradation by Trametes hirsuta strain Bm-2
title_fullStr	Laccase gene expression and vinasse biodegradation by Trametes hirsuta strain Bm-2
title_full_unstemmed	Laccase gene expression and vinasse biodegradation by Trametes hirsuta strain Bm-2
title_sort	laccase gene expression and vinasse biodegradation by trametes hirsuta strain bm-2
url	http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1096
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Laccase gene expression and vinasse biodegradation by Trametes hirsuta strain Bm-2

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