Supplementary material for Novel bioactive extract from yarrow obtained by the supercritical antisolvent-assisted technique inhibits lipid metabolism in colorectal cancer
Supplementary figure S1 | Chromatogram of the phenolic compounds of the UAE yarrow extract (λ = 280 nm). Supplementary figure S2 | Effect of UAE yarrow and yarrow Sep extracts in the inhibition of the gene expression of the master regulator of de novo lipogenesis SREBF1 in the metastasis CRC cell line SW620. Asterisks indicate statistically significant differences *p < 0.05; **p < 0.01; ***p < 0.005; and ****p < 0.001 relative to the control non-treated cells (three replicates, three independent experiments). Supplementary figure S3 | Effect of yarrow UAE and yarrow Sep in the mitochondrial oxidative phosphorylation of SW620 metastatic CRC cells. The basal respiration rate, spare respiratory capacity, ATP production, and proton leak of 8,000 cells per condition are compared. Data represent the mean ± SEM of four to six replicates. Supplementary figure S4 | Effect of UAE yarrow and yarrow Sep extracts in the expression of the epithelial markers E-cadherin in the metastatic CRC cell line SW620. Asterisks indicate statistically significant differences *p < 0.05; **p < 0.01; ***p < 0.005; and ****p < 0.001 relative to the control non-treated cells (three replicates, three independent experiments). Supplementary table S1 | List of oligos (forward and reverse) to assay lipid metabolism gene expression and epithelial to mesenchymal transition genes.
| Main Authors: | , , , , , |
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| Format: | dataset biblioteca |
| Language: | English |
| Published: |
Frontiers Media
2024
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| Online Access: | http://hdl.handle.net/10261/364334 |
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