Contribution of Proteins and Peptides to the Impact of a Soy Protein Isolate on Oxidative Stress and Inflammation-Associated Biomarkers in an Innate Immune Cell Model Inflammation-Associated Biomarkers in an Innate Immune Cell Model
The innate and adaptative immune systems are involved in the regulation of inflammatory and oxidative processes and mediators such as reactive oxygen species (ROS) and nitric oxide (NO). The exacerbated action of these players results in an oxidative stress status and chronic inflammation, which is responsible for the development of non-communicable diseases (NCDs). By modulating these mediators, bioactive compounds in food can exert a key role in the prevention of several NCDs. Among these compounds, soybean proteins and peptides such as lunasin have been considered to be among the most promising. The aim of this study was to obtain and characterize a soluble protein-enriched extract from a commercial soybean protein isolate and fractionate it into different fractions through ultrafiltration. Their antioxidant and immunomodulatory properties were then evaluated using biochemical and cell models. A total of 535 proteins (from 282 protein groups) were identified in the extract, in which the presence of the peptide lunasin was confirmed. The enrichment of this peptide was achieved in the 3¿10 kDa fraction. The protective effects against the oxidative stress induced by LPS in the macrophage model could have been mediated by the radical scavenging capacity of the peptides present in the soybean samples. Under basal conditions, the extract and its ultrafiltered fractions activated macrophages and induced the release of NO. However, under challenged conditions, the whole extract potentiated the NO-stimulating effects of LPS, whereas the fraction containing 3¿10 kDa peptides, including lunasin, counteracted the LPS-induced NO increase. Our findings suggest a promising role of soybean protein as an ingredient for functional foods and nutraceuticals aimed at promoting health and preventing oxidative stress and/or immune- alteration-associated diseases.
Main Authors: | , , , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Published: |
2023-05-17
|
Online Access: | http://hdl.handle.net/10261/346690 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cial-es-10261-346690 |
---|---|
record_format |
koha |
spelling |
dig-cial-es-10261-3466902024-02-12T09:30:13Z Contribution of Proteins and Peptides to the Impact of a Soy Protein Isolate on Oxidative Stress and Inflammation-Associated Biomarkers in an Innate Immune Cell Model Inflammation-Associated Biomarkers in an Innate Immune Cell Model Franca-Oliveira, Giselle Martinez-Rodriguez, Adolfo J. Morato, Esperanza Hernández-Ledesma, Blanca CSIC-UAM - Centro de Biología Molecular Severo Ochoa (CBM) Instituto de Salud Carlos III The innate and adaptative immune systems are involved in the regulation of inflammatory and oxidative processes and mediators such as reactive oxygen species (ROS) and nitric oxide (NO). The exacerbated action of these players results in an oxidative stress status and chronic inflammation, which is responsible for the development of non-communicable diseases (NCDs). By modulating these mediators, bioactive compounds in food can exert a key role in the prevention of several NCDs. Among these compounds, soybean proteins and peptides such as lunasin have been considered to be among the most promising. The aim of this study was to obtain and characterize a soluble protein-enriched extract from a commercial soybean protein isolate and fractionate it into different fractions through ultrafiltration. Their antioxidant and immunomodulatory properties were then evaluated using biochemical and cell models. A total of 535 proteins (from 282 protein groups) were identified in the extract, in which the presence of the peptide lunasin was confirmed. The enrichment of this peptide was achieved in the 3¿10 kDa fraction. The protective effects against the oxidative stress induced by LPS in the macrophage model could have been mediated by the radical scavenging capacity of the peptides present in the soybean samples. Under basal conditions, the extract and its ultrafiltered fractions activated macrophages and induced the release of NO. However, under challenged conditions, the whole extract potentiated the NO-stimulating effects of LPS, whereas the fraction containing 3¿10 kDa peptides, including lunasin, counteracted the LPS-induced NO increase. Our findings suggest a promising role of soybean protein as an ingredient for functional foods and nutraceuticals aimed at promoting health and preventing oxidative stress and/or immune- alteration-associated diseases. This research was funded by CSIC project 2019-AEP180. The proteomic analysis was carried out in the “CBMSO Protein Chemistry Facility” that belongs to ProteoRed and PRB3-ISCIII and was supported by grant PT17/0019 of the PE I+D+i 2013–2016, which was funded by ISCIII and ERDF 2024-02-12T09:30:12Z 2024-02-12T09:30:12Z 2023-05-17 2024-02-12T09:30:12Z artículo doi: 10.3390/plants12102011 issn: 2223-7747 Plants 12 (2023) http://hdl.handle.net/10261/346690 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/CSIC/2019-AEP180 info:eu-repo/grantAgreement/ISCIII/PRB3-ISCIII info:eu-repo/grantAgreement/ISCIII/ PT17/0019 of the PE I+D+i 2013–2016 Publisher's version http://dx.doi.org/10.3390/plants12102011 Sí open |
institution |
CIAL ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cial-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del CIAL España |
description |
The innate and adaptative immune systems are involved in the regulation of inflammatory
and oxidative processes and mediators such as reactive oxygen species (ROS) and nitric oxide (NO).
The exacerbated action of these players results in an oxidative stress status and chronic inflammation,
which is responsible for the development of non-communicable diseases (NCDs). By modulating
these mediators, bioactive compounds in food can exert a key role in the prevention of several
NCDs. Among these compounds, soybean proteins and peptides such as lunasin have been considered
to be among the most promising. The aim of this study was to obtain and characterize a soluble
protein-enriched extract from a commercial soybean protein isolate and fractionate it into different
fractions through ultrafiltration. Their antioxidant and immunomodulatory properties were then
evaluated using biochemical and cell models. A total of 535 proteins (from 282 protein groups) were
identified in the extract, in which the presence of the peptide lunasin was confirmed. The enrichment
of this peptide was achieved in the 3¿10 kDa fraction. The protective effects against the oxidative
stress induced by LPS in the macrophage model could have been mediated by the radical scavenging
capacity of the peptides present in the soybean samples. Under basal conditions, the extract
and its ultrafiltered fractions activated macrophages and induced the release of NO. However, under
challenged conditions, the whole extract potentiated the NO-stimulating effects of LPS, whereas
the fraction containing 3¿10 kDa peptides, including lunasin, counteracted the LPS-induced NO
increase. Our findings suggest a promising role of soybean protein as an ingredient for functional
foods and nutraceuticals aimed at promoting health and preventing oxidative stress and/or immune-
alteration-associated diseases. |
author2 |
CSIC-UAM - Centro de Biología Molecular Severo Ochoa (CBM) |
author_facet |
CSIC-UAM - Centro de Biología Molecular Severo Ochoa (CBM) Franca-Oliveira, Giselle Martinez-Rodriguez, Adolfo J. Morato, Esperanza Hernández-Ledesma, Blanca |
format |
artículo |
author |
Franca-Oliveira, Giselle Martinez-Rodriguez, Adolfo J. Morato, Esperanza Hernández-Ledesma, Blanca |
spellingShingle |
Franca-Oliveira, Giselle Martinez-Rodriguez, Adolfo J. Morato, Esperanza Hernández-Ledesma, Blanca Contribution of Proteins and Peptides to the Impact of a Soy Protein Isolate on Oxidative Stress and Inflammation-Associated Biomarkers in an Innate Immune Cell Model Inflammation-Associated Biomarkers in an Innate Immune Cell Model |
author_sort |
Franca-Oliveira, Giselle |
title |
Contribution of Proteins and Peptides to the Impact of a Soy
Protein Isolate on Oxidative Stress and Inflammation-Associated Biomarkers in an Innate Immune
Cell Model
Inflammation-Associated Biomarkers in an Innate Immune
Cell Model |
title_short |
Contribution of Proteins and Peptides to the Impact of a Soy
Protein Isolate on Oxidative Stress and Inflammation-Associated Biomarkers in an Innate Immune
Cell Model
Inflammation-Associated Biomarkers in an Innate Immune
Cell Model |
title_full |
Contribution of Proteins and Peptides to the Impact of a Soy
Protein Isolate on Oxidative Stress and Inflammation-Associated Biomarkers in an Innate Immune
Cell Model
Inflammation-Associated Biomarkers in an Innate Immune
Cell Model |
title_fullStr |
Contribution of Proteins and Peptides to the Impact of a Soy
Protein Isolate on Oxidative Stress and Inflammation-Associated Biomarkers in an Innate Immune
Cell Model
Inflammation-Associated Biomarkers in an Innate Immune
Cell Model |
title_full_unstemmed |
Contribution of Proteins and Peptides to the Impact of a Soy
Protein Isolate on Oxidative Stress and Inflammation-Associated Biomarkers in an Innate Immune
Cell Model
Inflammation-Associated Biomarkers in an Innate Immune
Cell Model |
title_sort |
contribution of proteins and peptides to the impact of a soy
protein isolate on oxidative stress and inflammation-associated biomarkers in an innate immune
cell model
inflammation-associated biomarkers in an innate immune
cell model |
publishDate |
2023-05-17 |
url |
http://hdl.handle.net/10261/346690 |
work_keys_str_mv |
AT francaoliveiragiselle contributionofproteinsandpeptidestotheimpactofasoyproteinisolateonoxidativestressandinflammationassociatedbiomarkersinaninnateimmunecellmodelinflammationassociatedbiomarkersinaninnateimmunecellmodel AT martinezrodriguezadolfoj contributionofproteinsandpeptidestotheimpactofasoyproteinisolateonoxidativestressandinflammationassociatedbiomarkersinaninnateimmunecellmodelinflammationassociatedbiomarkersinaninnateimmunecellmodel AT moratoesperanza contributionofproteinsandpeptidestotheimpactofasoyproteinisolateonoxidativestressandinflammationassociatedbiomarkersinaninnateimmunecellmodelinflammationassociatedbiomarkersinaninnateimmunecellmodel AT hernandezledesmablanca contributionofproteinsandpeptidestotheimpactofasoyproteinisolateonoxidativestressandinflammationassociatedbiomarkersinaninnateimmunecellmodelinflammationassociatedbiomarkersinaninnateimmunecellmodel |
_version_ |
1792484928454131712 |