Mechanisms for the interaction of the milk fat globule membrane with the plasma membrane of gut epithelial cells
9 Páginas.-- 4 Figuras.-- 1 Tabla
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| Format: | artículo biblioteca |
| Language: | English |
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Elsevier
2023-11
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| Subjects: | Caco-2, Cellular membrane, Fluorescence, Fusion, Microscopy, Milk fat globule membrane, Milk lipids, |
| Online Access: | http://hdl.handle.net/10261/339070 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85166958704 |
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dig-cial-es-10261-3390702024-05-16T20:39:28Z Mechanisms for the interaction of the milk fat globule membrane with the plasma membrane of gut epithelial cells Martínez-Sánchez, Victoria Calvo, Maria V. Viera Alcaide, Isabel Girón-Calle, Julio Fontecha, F. Javier Pérez Gálvez, Antonio Ministerio de Ciencia e Innovación (España) European Commission Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Caco-2 Cellular membrane Fluorescence Fusion Microscopy Milk fat globule membrane Milk lipids 9 Páginas.-- 4 Figuras.-- 1 Tabla The milk fat globule membrane (MFGM) provides infants and adults with several health benefits. These are not derived solely from its unique composition, but also from arrangement of lipids in the MFGM that, in the case of newborns, could reach the intestine partially intact. Fluorochromes associated with lipid derivatives were used to prove a fusion process between the MFGM and the cellular membrane of differentiated Caco-2 cells. To explore the mechanism of this interaction, incubations of MFGM with Caco-2 cells were carried out in the presence of fusogenic agents or compounds that block other MFGM interaction pathways with cells. Confocal fluorescence microscopy provided visual evidence of the fusion process. Lastly, determination on the lipid profile of cells after their interaction with MFGM indicated a metabolic rearrangement of lipids leading to accumulation of triacylglycerols. This work was supported by the Ministerio de Ciencia e Innovación, MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe” (project PID2020-114821RB-I00). Peer reviewed 2023-11-16T11:27:06Z 2023-11-16T11:27:06Z 2023-11 artículo http://purl.org/coar/resource_type/c_6501 Food Research International 173(1): 113330 (2023) 0963-9969 http://hdl.handle.net/10261/339070 10.1016/j.foodres.2023.113330 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 37803640 2-s2.0-85166958704 https://api.elsevier.com/content/abstract/scopus_id/85166958704 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-114821RB-I00/ES/IMPACTO DE UN SUPLEMENTO ENRIQUECIDO EN MEMBRANA DEL GLOBULO GRASO LACTEO EN LA SALUD MEDIANTE ESTUDIOS IN VITRO E IN VIVO Y SUS POTENCIALES MECANISMOS DE ACCION/ Publisher's version https://doi.org/10.1016/j.foodres.2023.113330 Sí open application/pdf Elsevier |
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Caco-2 Cellular membrane Fluorescence Fusion Microscopy Milk fat globule membrane Milk lipids Caco-2 Cellular membrane Fluorescence Fusion Microscopy Milk fat globule membrane Milk lipids |
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Caco-2 Cellular membrane Fluorescence Fusion Microscopy Milk fat globule membrane Milk lipids Caco-2 Cellular membrane Fluorescence Fusion Microscopy Milk fat globule membrane Milk lipids Martínez-Sánchez, Victoria Calvo, Maria V. Viera Alcaide, Isabel Girón-Calle, Julio Fontecha, F. Javier Pérez Gálvez, Antonio Mechanisms for the interaction of the milk fat globule membrane with the plasma membrane of gut epithelial cells |
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9 Páginas.-- 4 Figuras.-- 1 Tabla |
| author2 |
Ministerio de Ciencia e Innovación (España) |
| author_facet |
Ministerio de Ciencia e Innovación (España) Martínez-Sánchez, Victoria Calvo, Maria V. Viera Alcaide, Isabel Girón-Calle, Julio Fontecha, F. Javier Pérez Gálvez, Antonio |
| format |
artículo |
| topic_facet |
Caco-2 Cellular membrane Fluorescence Fusion Microscopy Milk fat globule membrane Milk lipids |
| author |
Martínez-Sánchez, Victoria Calvo, Maria V. Viera Alcaide, Isabel Girón-Calle, Julio Fontecha, F. Javier Pérez Gálvez, Antonio |
| author_sort |
Martínez-Sánchez, Victoria |
| title |
Mechanisms for the interaction of the milk fat globule membrane with the plasma membrane of gut epithelial cells |
| title_short |
Mechanisms for the interaction of the milk fat globule membrane with the plasma membrane of gut epithelial cells |
| title_full |
Mechanisms for the interaction of the milk fat globule membrane with the plasma membrane of gut epithelial cells |
| title_fullStr |
Mechanisms for the interaction of the milk fat globule membrane with the plasma membrane of gut epithelial cells |
| title_full_unstemmed |
Mechanisms for the interaction of the milk fat globule membrane with the plasma membrane of gut epithelial cells |
| title_sort |
mechanisms for the interaction of the milk fat globule membrane with the plasma membrane of gut epithelial cells |
| publisher |
Elsevier |
| publishDate |
2023-11 |
| url |
http://hdl.handle.net/10261/339070 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85166958704 |
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