Combining UV Irradiation and Alkaline Deacetylation to Obtain Vitamin D- and Chitosan-Enriched Fractions from Shiitake Mushrooms (Lentinula edodes)
Shiitake mushrooms (Lentinula edodes) contain compounds with hypocholesterolemic and immune-modulatory activities such as ergosterol and chitin that can be partially transformed into vitamin D and chitosan to yield extracts with enhanced bioactivities. A method was optimized to increase vitamin D2 levels by irradiating a non-soluble fraction obtained from shiitake mushrooms during 1 h with UV-light (254 nm) at 50 °C in methanol. After 1 h, almost all of the ergosterol was transformed into vitamin D2. The conversion ratio can be simply adjusted by reducing the irradiation time. A deacetylation treatment with 50% NaOH for 24 h at 95 °C was needed to generate chitosan and other water-soluble lower molecular weight derivatives from chitin. To obtain an extract enriched in both compounds, the defined methods can be combined, but the extracts should be firstly deacetylated and later irradiated. In this order, an extract is obtained containing vitamin D2 (4.65 mg/g) and chitosan (2.83%).
Main Authors: | Morales, Diego, Jiménez Piris, Adriana, Ruiz-Rodríguez, Alejandro, Soler-Rivas, Cristina |
---|---|
Other Authors: | Ministerio de Ciencia, Innovación y Universidades (España) |
Format: | artículo biblioteca |
Language: | English |
Published: |
Springer Nature
2023
|
Online Access: | http://hdl.handle.net/10261/311357 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Vitamin D-enriched extracts obtained from shiitake mushrooms (Lentinula edodes) by supercritical fluid extraction and UV-irradiation
by: Morales, Diego, et al.
Published: (2017) -
In vitro and in vivo testing of the hypocholesterolemic activity of ergosterol- and β-glucan-enriched extracts obtained from shiitake mushrooms (Lentinula edodes)
by: Morales, Diego, et al.
Published: (2019) -
Production of a β-d-glucan-rich extract from Shiitake mushrooms (Lentinula edodes) by an extraction/microfiltration/reverse osmosis (nanofiltration) process
by: Morales, Diego, et al.
Published: (2019) -
Isolation and comparison of α- and β-D-glucans from shiitake mushrooms (Lentinula edodes) with different biological activities
by: Morales, Diego, et al.
Published: (2020) -
Improvement of nutritional composition of shiitake mushroom (Lentinula edodes) using formulated substrates of plant and animal origins
by: Desisa, Buzayehu, et al.
Published: (2024-06-01)