Combining UV Irradiation and Alkaline Deacetylation to Obtain Vitamin D- and Chitosan-Enriched Fractions from Shiitake Mushrooms (Lentinula edodes)
Shiitake mushrooms (Lentinula edodes) contain compounds with hypocholesterolemic and immune-modulatory activities such as ergosterol and chitin that can be partially transformed into vitamin D and chitosan to yield extracts with enhanced bioactivities. A method was optimized to increase vitamin D2 levels by irradiating a non-soluble fraction obtained from shiitake mushrooms during 1 h with UV-light (254 nm) at 50 °C in methanol. After 1 h, almost all of the ergosterol was transformed into vitamin D2. The conversion ratio can be simply adjusted by reducing the irradiation time. A deacetylation treatment with 50% NaOH for 24 h at 95 °C was needed to generate chitosan and other water-soluble lower molecular weight derivatives from chitin. To obtain an extract enriched in both compounds, the defined methods can be combined, but the extracts should be firstly deacetylated and later irradiated. In this order, an extract is obtained containing vitamin D2 (4.65 mg/g) and chitosan (2.83%).
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2023
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dig-cial-es-10261-3113572023-06-13T11:37:47Z Combining UV Irradiation and Alkaline Deacetylation to Obtain Vitamin D- and Chitosan-Enriched Fractions from Shiitake Mushrooms (Lentinula edodes) Morales, Diego Jiménez Piris, Adriana Ruiz-Rodríguez, Alejandro Soler-Rivas, Cristina Ministerio de Ciencia, Innovación y Universidades (España) Ministerio de Ciencia e Innovación (España) Comunidad de Madrid Agencia Estatal de Investigación (España) Shiitake mushrooms (Lentinula edodes) contain compounds with hypocholesterolemic and immune-modulatory activities such as ergosterol and chitin that can be partially transformed into vitamin D and chitosan to yield extracts with enhanced bioactivities. A method was optimized to increase vitamin D2 levels by irradiating a non-soluble fraction obtained from shiitake mushrooms during 1 h with UV-light (254 nm) at 50 °C in methanol. After 1 h, almost all of the ergosterol was transformed into vitamin D2. The conversion ratio can be simply adjusted by reducing the irradiation time. A deacetylation treatment with 50% NaOH for 24 h at 95 °C was needed to generate chitosan and other water-soluble lower molecular weight derivatives from chitin. To obtain an extract enriched in both compounds, the defined methods can be combined, but the extracts should be firstly deacetylated and later irradiated. In this order, an extract is obtained containing vitamin D2 (4.65 mg/g) and chitosan (2.83%). This research was supported by the national R + D program from the Spanish Ministry of Science and Innovation (project AGL2014-56211-R) and the regional program from the Community of Madrid, Spain (S2013/ABI-2728). D. M. received from the Spanish Ministry of Science and Innovation a Juan de la Cierva Formación scholarship (FJC2020-044585-I). Peer reviewed 2023-06-13T11:37:46Z 2023-06-13T11:37:46Z 2023 artículo Food and Bioprocess Technology 16: 1303-1311 (2023) http://hdl.handle.net/10261/311357 10.1007/s11947-023-02998-2 1935-5149 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO//AGL2014-56211-R/ES/AJUSTE Y ESCALADO DE LOS ALIMENTOS HIPOCOLESTEROLEMICOS DISEÑADOS A BASE DE EXTRACTOS DE HONGOS COMESTIBLES Y VALIDACION CON ESTUDIOS CLINICOS/ S2013/ABI-2728/ALIBIRD-CM info:eu-repo/grantAgreement/AEI//FJC2020-044585-I https://doi.org/10.1007/s11947-023-02998-2 Sí none Springer Nature |
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Shiitake mushrooms (Lentinula edodes) contain compounds with hypocholesterolemic and immune-modulatory activities such as ergosterol and chitin that can be partially transformed into vitamin D and chitosan to yield extracts with enhanced bioactivities. A method was optimized to increase vitamin D2 levels by irradiating a non-soluble fraction obtained from shiitake mushrooms during 1 h with UV-light (254 nm) at 50 °C in methanol. After 1 h, almost all of the ergosterol was transformed into vitamin D2. The conversion ratio can be simply adjusted by reducing the irradiation time. A deacetylation treatment with 50% NaOH for 24 h at 95 °C was needed to generate chitosan and other water-soluble lower molecular weight derivatives from chitin. To obtain an extract enriched in both compounds, the defined methods can be combined, but the extracts should be firstly deacetylated and later irradiated. In this order, an extract is obtained containing vitamin D2 (4.65 mg/g) and chitosan (2.83%). |
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Ministerio de Ciencia, Innovación y Universidades (España) |
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Ministerio de Ciencia, Innovación y Universidades (España) Morales, Diego Jiménez Piris, Adriana Ruiz-Rodríguez, Alejandro Soler-Rivas, Cristina |
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Morales, Diego Jiménez Piris, Adriana Ruiz-Rodríguez, Alejandro Soler-Rivas, Cristina |
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Morales, Diego Jiménez Piris, Adriana Ruiz-Rodríguez, Alejandro Soler-Rivas, Cristina Combining UV Irradiation and Alkaline Deacetylation to Obtain Vitamin D- and Chitosan-Enriched Fractions from Shiitake Mushrooms (Lentinula edodes) |
author_sort |
Morales, Diego |
title |
Combining UV Irradiation and Alkaline Deacetylation to Obtain Vitamin D- and Chitosan-Enriched Fractions from Shiitake Mushrooms (Lentinula edodes) |
title_short |
Combining UV Irradiation and Alkaline Deacetylation to Obtain Vitamin D- and Chitosan-Enriched Fractions from Shiitake Mushrooms (Lentinula edodes) |
title_full |
Combining UV Irradiation and Alkaline Deacetylation to Obtain Vitamin D- and Chitosan-Enriched Fractions from Shiitake Mushrooms (Lentinula edodes) |
title_fullStr |
Combining UV Irradiation and Alkaline Deacetylation to Obtain Vitamin D- and Chitosan-Enriched Fractions from Shiitake Mushrooms (Lentinula edodes) |
title_full_unstemmed |
Combining UV Irradiation and Alkaline Deacetylation to Obtain Vitamin D- and Chitosan-Enriched Fractions from Shiitake Mushrooms (Lentinula edodes) |
title_sort |
combining uv irradiation and alkaline deacetylation to obtain vitamin d- and chitosan-enriched fractions from shiitake mushrooms (lentinula edodes) |
publisher |
Springer Nature |
publishDate |
2023 |
url |
http://hdl.handle.net/10261/311357 |
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