Functional profiling of allergen-specific effector CD4+ T cells in mouse models of food allergy

Functional profiling of allergen-specific effector CD4+ T cells in mouse models of food allergy

Trabajo presentado al Congress of the American Academy of Allergy, Asthma and Immunology - AAAAI Annual Meeting, celebrado en San Antonio,TX (USA) del 24 al 27 de febrero de 2023.

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Main Authors: Menchén-Martínez, David, Pérez-Rodríguez, Leticia, López-Fandiño, Rosina, Molina, Elena
Format: artículo biblioteca
Language:English
Published: Elsevier 2023
Online Access:http://hdl.handle.net/10261/309462
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spelling dig-cial-es-10261-3094622024-05-08T10:34:57Z Functional profiling of allergen-specific effector CD4+ T cells in mouse models of food allergy Menchén-Martínez, David Pérez-Rodríguez, Leticia López-Fandiño, Rosina Molina, Elena Trabajo presentado al Congress of the American Academy of Allergy, Asthma and Immunology - AAAAI Annual Meeting, celebrado en San Antonio,TX (USA) del 24 al 27 de febrero de 2023. [Rationale] Allergen-specific T cells have demonstrated to be crucial in the course of food allergy. We hypothesized that the identification of allergen-specific T cells by using activation markers may allow their monitoring at effector sites. The aim of this study is to identify the functional profile of allergen-specific T cells a murine model of food allergy. [Methods] C57BL/6J mice were orally sensitized to peanut using cholera toxin as an adjuvant. Cells from different organs were isolated, rested overnight and then restimulated in vitro with peanut for 6h. The upregulation of activation markers (CD154, CD69, and CD137) analyzed by flow cytometry was used to identify allergen-specific T cells. The phenotypical and functional profile was characterized by the expression of IL-4, IFN, IL-10, CD103, and CCR9. [Results] Peanut-specific T cells were identified as CD3+CD4+CD44+CD154+ and they were significantly increased in all the organs studied. Spleen CD154+ T cells were significantly correlated with peanut-specific IgG1 levels (Pearson r=0,862). Peanut-specific CD154+ T cells showed a remarkable Th2 phenotype as shown by their increased expression of IL-4. Our results revealed that peanut-specific T cells migrate from the amina propria (0.450 ± 0.070% of CD154+ in memory T cells) to distal axillary lymph nodes (0.085 ± 0,025% of CD154+ in memory T cells) based on their increased expression of gut homing marker CCR9 (56.230 ± 9.812% of CCR9+ cells in memory CD154+ T cells). [Conclusions] Our results conclude that allergen-specific effector T cells migrate from intestinal mucosa to secondary lymphoid organs, exerting their pathogenic effect by the secretion of IL-4. Peer reviewed 2023-05-23T09:50:44Z 2023-05-23T09:50:44Z 2023 artículo http://purl.org/coar/resource_type/c_5794 Journal of Allergy and Clinical Immunology 151(2Suppl.): AB176 (2023) 0091-6749 http://hdl.handle.net/10261/309462 10.1016/j.jaci.2022.12.551 en https://doi.org/10.1016/j.jaci.2022.12.551 Sí none Elsevier
institution CIAL ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-cial-es
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region Europa del Sur
libraryname Biblioteca del CIAL España
language English
description Trabajo presentado al Congress of the American Academy of Allergy, Asthma and Immunology - AAAAI Annual Meeting, celebrado en San Antonio,TX (USA) del 24 al 27 de febrero de 2023.
format artículo
author Menchén-Martínez, David
Pérez-Rodríguez, Leticia
López-Fandiño, Rosina
Molina, Elena
spellingShingle Menchén-Martínez, David
Pérez-Rodríguez, Leticia
López-Fandiño, Rosina
Molina, Elena
Functional profiling of allergen-specific effector CD4+ T cells in mouse models of food allergy
author_facet Menchén-Martínez, David
Pérez-Rodríguez, Leticia
López-Fandiño, Rosina
Molina, Elena
author_sort Menchén-Martínez, David
title Functional profiling of allergen-specific effector CD4+ T cells in mouse models of food allergy
title_short Functional profiling of allergen-specific effector CD4+ T cells in mouse models of food allergy
title_full Functional profiling of allergen-specific effector CD4+ T cells in mouse models of food allergy
title_fullStr Functional profiling of allergen-specific effector CD4+ T cells in mouse models of food allergy
title_full_unstemmed Functional profiling of allergen-specific effector CD4+ T cells in mouse models of food allergy
title_sort functional profiling of allergen-specific effector cd4+ t cells in mouse models of food allergy
publisher Elsevier
publishDate 2023
url http://hdl.handle.net/10261/309462
work_keys_str_mv AT menchenmartinezdavid functionalprofilingofallergenspecificeffectorcd4tcellsinmousemodelsoffoodallergy
AT perezrodriguezleticia functionalprofilingofallergenspecificeffectorcd4tcellsinmousemodelsoffoodallergy
AT lopezfandinorosina functionalprofilingofallergenspecificeffectorcd4tcellsinmousemodelsoffoodallergy
AT molinaelena functionalprofilingofallergenspecificeffectorcd4tcellsinmousemodelsoffoodallergy
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