Strain-specific inhibition of the adherence of uropathogenic bacteria to bladder cells by probiotic Lactobacillus spp.
Urinary tract infections (UTIs), one of most common infections worldwide, face high recurrence rates and increasing antimicrobial resistance. Probiotic bacteria, especially of the genus Lactobacillus, are considered a promising preventive and/or treatment therapy against UTIs. In order to elucidate the mechanisms involved in these beneficial effects, we studied the impact of different Lactobacillus strains (Lactobacillus salivarius UCM572, L. plantarum CLC17 and L. acidophilus 01) in the adherence of reference and clinical uropathogenic strains (Escherichia coli ATCC® 53503, E. coli 10791, Enterococcus faecalis 04-1, En. faecalis 08-1 and Staphylococcus epidermidis 08-3) to T24 epithelial bladder cells. In general, the Lactobacillus strains with previous in vivo evidence of beneficial effects against UTIs (L. salivarius UCM572 and L. acidophilus 01) significantly inhibited the adherence of the five uropathogens to T24 cells, displaying percentages of inhibition ranging between 22.2% and 43.9%, and between 16.5% and 53.7%, respectively. On the other hand, L. plantarum CLC17, a strain with no expected effects on UTIs, showed almost negligible anti-adherence effects.Therefore, these in vitro results suggest that inhibition of the adherence of uropathogens to epithelial bladder cells may be one of the mechanisms involved in the potential beneficial effects of probiotics against UTIs in vivo.
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Format: | artículo biblioteca |
Language: | English |
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Oxford University Press
2017
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Subjects: | UTI, Uropathogens, Probiotics, Lactobacillus, Bladder cells, Bacteria adherence, |
Online Access: | http://hdl.handle.net/10261/194227 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100003329 |
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dig-cial-es-10261-1942272019-11-09T02:07:48Z Strain-specific inhibition of the adherence of uropathogenic bacteria to bladder cells by probiotic Lactobacillus spp. González de Llano, Dolores Arroyo, Amalia Cárdenas, Nivia Rodríguez, Juan M. Moreno-Arribas, M. Victoria Bartolomé, Begoña Ministerio de Economía y Competitividad (España) Comunidad de Madrid UTI Uropathogens Probiotics Lactobacillus Bladder cells Bacteria adherence Urinary tract infections (UTIs), one of most common infections worldwide, face high recurrence rates and increasing antimicrobial resistance. Probiotic bacteria, especially of the genus Lactobacillus, are considered a promising preventive and/or treatment therapy against UTIs. In order to elucidate the mechanisms involved in these beneficial effects, we studied the impact of different Lactobacillus strains (Lactobacillus salivarius UCM572, L. plantarum CLC17 and L. acidophilus 01) in the adherence of reference and clinical uropathogenic strains (Escherichia coli ATCC® 53503, E. coli 10791, Enterococcus faecalis 04-1, En. faecalis 08-1 and Staphylococcus epidermidis 08-3) to T24 epithelial bladder cells. In general, the Lactobacillus strains with previous in vivo evidence of beneficial effects against UTIs (L. salivarius UCM572 and L. acidophilus 01) significantly inhibited the adherence of the five uropathogens to T24 cells, displaying percentages of inhibition ranging between 22.2% and 43.9%, and between 16.5% and 53.7%, respectively. On the other hand, L. plantarum CLC17, a strain with no expected effects on UTIs, showed almost negligible anti-adherence effects.Therefore, these in vitro results suggest that inhibition of the adherence of uropathogens to epithelial bladder cells may be one of the mechanisms involved in the potential beneficial effects of probiotics against UTIs in vivo. This work was supported by the Spanish Ministry of Industry, Economy and Competitiveness (AGL2015-64522-C2-1-R, AGL2016-75476-R) and the Comunidad de Madrid (ALIBIRD S2013/ABI-2728). Peer reviewed 2019-11-08T13:13:48Z 2019-11-08T13:13:48Z 2017 artículo http://purl.org/coar/resource_type/c_6501 Pathogens and Disease 75(4): ftx043 (2017) http://hdl.handle.net/10261/194227 10.1093/femspd/ftx043 2049-632X http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100003329 28402532 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-64522-C2-1-R info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-75476-R S2013/ABI-2728/ALIBIRD https://doi.org/10.1093/femspd/ftx043 Sí none Oxford University Press |
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UTI Uropathogens Probiotics Lactobacillus Bladder cells Bacteria adherence UTI Uropathogens Probiotics Lactobacillus Bladder cells Bacteria adherence |
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UTI Uropathogens Probiotics Lactobacillus Bladder cells Bacteria adherence UTI Uropathogens Probiotics Lactobacillus Bladder cells Bacteria adherence González de Llano, Dolores Arroyo, Amalia Cárdenas, Nivia Rodríguez, Juan M. Moreno-Arribas, M. Victoria Bartolomé, Begoña Strain-specific inhibition of the adherence of uropathogenic bacteria to bladder cells by probiotic Lactobacillus spp. |
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Urinary tract infections (UTIs), one of most common infections worldwide, face high recurrence rates and increasing antimicrobial resistance. Probiotic bacteria, especially of the genus Lactobacillus, are considered a promising preventive and/or treatment therapy against UTIs. In order to elucidate the mechanisms involved in these beneficial effects, we studied the impact of different Lactobacillus strains (Lactobacillus salivarius UCM572, L. plantarum CLC17 and L. acidophilus 01) in the adherence of reference and clinical uropathogenic strains (Escherichia coli ATCC® 53503, E. coli 10791, Enterococcus faecalis 04-1, En. faecalis 08-1 and Staphylococcus epidermidis 08-3) to T24 epithelial bladder cells. In general, the Lactobacillus strains with previous in vivo evidence of beneficial effects against UTIs (L. salivarius UCM572 and L. acidophilus 01) significantly inhibited the adherence of the five uropathogens to T24 cells, displaying percentages of inhibition ranging between 22.2% and 43.9%, and between 16.5% and 53.7%, respectively. On the other hand, L. plantarum CLC17, a strain with no expected effects on UTIs, showed almost negligible anti-adherence effects.Therefore, these in vitro results suggest that inhibition of the adherence of uropathogens to epithelial bladder cells may be one of the mechanisms involved in the potential beneficial effects of probiotics against UTIs in vivo. |
author2 |
Ministerio de Economía y Competitividad (España) |
author_facet |
Ministerio de Economía y Competitividad (España) González de Llano, Dolores Arroyo, Amalia Cárdenas, Nivia Rodríguez, Juan M. Moreno-Arribas, M. Victoria Bartolomé, Begoña |
format |
artículo |
topic_facet |
UTI Uropathogens Probiotics Lactobacillus Bladder cells Bacteria adherence |
author |
González de Llano, Dolores Arroyo, Amalia Cárdenas, Nivia Rodríguez, Juan M. Moreno-Arribas, M. Victoria Bartolomé, Begoña |
author_sort |
González de Llano, Dolores |
title |
Strain-specific inhibition of the adherence of uropathogenic bacteria to bladder cells by probiotic Lactobacillus spp. |
title_short |
Strain-specific inhibition of the adherence of uropathogenic bacteria to bladder cells by probiotic Lactobacillus spp. |
title_full |
Strain-specific inhibition of the adherence of uropathogenic bacteria to bladder cells by probiotic Lactobacillus spp. |
title_fullStr |
Strain-specific inhibition of the adherence of uropathogenic bacteria to bladder cells by probiotic Lactobacillus spp. |
title_full_unstemmed |
Strain-specific inhibition of the adherence of uropathogenic bacteria to bladder cells by probiotic Lactobacillus spp. |
title_sort |
strain-specific inhibition of the adherence of uropathogenic bacteria to bladder cells by probiotic lactobacillus spp. |
publisher |
Oxford University Press |
publishDate |
2017 |
url |
http://hdl.handle.net/10261/194227 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100003329 |
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