Pectin characterisation using size exclusion chromatography: A comparison of ELS and RI detection
A high-performance size-exclusion chromatography (HPSEC) method coupled to Evaporative Light Scattering (ELS) and Refractive Index (RI) detectors were evaluated and compared for the molecular mass (Mw) estimation of pectin in a wide range (0.342–805 kDa). Instrumental parameters of the ELSD were optimised by Response Surface Methodology (RSM) being 73 °C the evaporator temperature and 0.9 mL/min the air flow rate. The linear range for the ELSD concentration response was wider (10–2250 mg/L) and better (R = 0.985) than RID (10–1500 mg/L; R = 0.875). The limits of detection (LOD) and quantitation (LOQ) for all pullulans hardly changed in ELSD (LOD: 1.22–1.99 mg/L; LOQ: 4.07–6.63 mg/L); however, RID showed huge variations (LOD: 0.49–10.41 mg/L; LOQ: 1.64–34.70 mg/L), which increased with the Mw. In general, responses of both detectors were similar for the Mw estimation, although pectin characterisation with HPSEC-ELSD exhibited better results in the lowest Mw compounds.
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Published: |
Elsevier
2018
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| Subjects: | Light scattering, Response surface methodology, Pectin, Molecular mass, Refraction index, |
| Online Access: | http://hdl.handle.net/10261/171038 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100012818 |
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