Enzymatic strategies for solvent-free production of short and medium chain phytosteryl esters
Transesterification of phytosterols with short and medium chain fatty acids (MCFAs) ethyl esters and with tributyrin catalyzed by Candida rugosa and Pseudomonas stutzeri TL lipases in solvent-free medium was studied including the effects of temperature, fatty acid specificity, enzyme quantity, the residual activity of each lipase and reduced pressure. More than the 90% of the original phytosterols were transformed into their corresponding phystosteryl esters after 3-4h reaction at 50-60°C and at 100-350mbar. The product mixtures containing 40-50% (w/w) phystosteryl esters were then vacuum distilled to remove molar excess of SCFA and MCFA ethyl esters utilized in the enzymatic reaction. By this methodology, purities >95% in short and medium chain phytosteryl esters were finally obtained. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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Wiley-VCH
2012
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Subjects: | Phytosteryl ester, Candida rugosa lipase, Butyric acid, Pseudomonas stutzeri TL lipase, |
Online Access: | http://hdl.handle.net/10261/101300 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 |
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dig-cial-es-10261-1013002018-09-18T12:13:18Z Enzymatic strategies for solvent-free production of short and medium chain phytosteryl esters Torrelo, Guzmán Torres, Carlos F. Reglero, Guillermo Comunidad de Madrid Ministerio de Ciencia e Innovación (España) Phytosteryl ester Candida rugosa lipase Butyric acid Pseudomonas stutzeri TL lipase Transesterification of phytosterols with short and medium chain fatty acids (MCFAs) ethyl esters and with tributyrin catalyzed by Candida rugosa and Pseudomonas stutzeri TL lipases in solvent-free medium was studied including the effects of temperature, fatty acid specificity, enzyme quantity, the residual activity of each lipase and reduced pressure. More than the 90% of the original phytosterols were transformed into their corresponding phystosteryl esters after 3-4h reaction at 50-60°C and at 100-350mbar. The product mixtures containing 40-50% (w/w) phystosteryl esters were then vacuum distilled to remove molar excess of SCFA and MCFA ethyl esters utilized in the enzymatic reaction. By this methodology, purities >95% in short and medium chain phytosteryl esters were finally obtained. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Funded by: Comunidad Autónoma de Madrid (ALIBIRD. Grant Number: S2009/AGR-1469 and Consolider-Ingenio FUN-C-FOOD. Grant Number: CSD2007-00063. Peer Reviewed 2014-08-27T12:16:30Z 2014-08-27T12:16:30Z 2012 2014-08-27T12:16:30Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1002/ejlt.201100346 issn: 1438-7697 e-issn: 1438-9312 European Journal of Lipid Science and Technology 114(6): 670-676 (2012) http://hdl.handle.net/10261/101300 10.1002/ejlt.201100346 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 #PLACEHOLDER_PARENT_METADATA_VALUE# S2009/AGR-1469/ALIBIRD none Wiley-VCH |
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Phytosteryl ester Candida rugosa lipase Butyric acid Pseudomonas stutzeri TL lipase Phytosteryl ester Candida rugosa lipase Butyric acid Pseudomonas stutzeri TL lipase |
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Phytosteryl ester Candida rugosa lipase Butyric acid Pseudomonas stutzeri TL lipase Phytosteryl ester Candida rugosa lipase Butyric acid Pseudomonas stutzeri TL lipase Torrelo, Guzmán Torres, Carlos F. Reglero, Guillermo Enzymatic strategies for solvent-free production of short and medium chain phytosteryl esters |
description |
Transesterification of phytosterols with short and medium chain fatty acids (MCFAs) ethyl esters and with tributyrin catalyzed by Candida rugosa and Pseudomonas stutzeri TL lipases in solvent-free medium was studied including the effects of temperature, fatty acid specificity, enzyme quantity, the residual activity of each lipase and reduced pressure. More than the 90% of the original phytosterols were transformed into their corresponding phystosteryl esters after 3-4h reaction at 50-60°C and at 100-350mbar. The product mixtures containing 40-50% (w/w) phystosteryl esters were then vacuum distilled to remove molar excess of SCFA and MCFA ethyl esters utilized in the enzymatic reaction. By this methodology, purities >95% in short and medium chain phytosteryl esters were finally obtained. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
author2 |
Comunidad de Madrid |
author_facet |
Comunidad de Madrid Torrelo, Guzmán Torres, Carlos F. Reglero, Guillermo |
format |
artículo |
topic_facet |
Phytosteryl ester Candida rugosa lipase Butyric acid Pseudomonas stutzeri TL lipase |
author |
Torrelo, Guzmán Torres, Carlos F. Reglero, Guillermo |
author_sort |
Torrelo, Guzmán |
title |
Enzymatic strategies for solvent-free production of short and medium chain phytosteryl esters |
title_short |
Enzymatic strategies for solvent-free production of short and medium chain phytosteryl esters |
title_full |
Enzymatic strategies for solvent-free production of short and medium chain phytosteryl esters |
title_fullStr |
Enzymatic strategies for solvent-free production of short and medium chain phytosteryl esters |
title_full_unstemmed |
Enzymatic strategies for solvent-free production of short and medium chain phytosteryl esters |
title_sort |
enzymatic strategies for solvent-free production of short and medium chain phytosteryl esters |
publisher |
Wiley-VCH |
publishDate |
2012 |
url |
http://hdl.handle.net/10261/101300 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 |
work_keys_str_mv |
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1777671154709823488 |