Antiviral compounds obtained from microalgae commonly used as carotenoid sources
Pressurized liquid extraction (PLE), an environmentally friendly technique, has been used to obtain antiviral compounds from microalgae commonly used as carotenoid sources: Haematococcus pluvialis and Dunaliella salina. The antiviral properties of PLE extracts (hexane, ethanol and water) were evaluated against herpes simplex virus type 1 (HSV-1) at different stages during viral infection. Pretreatment of Vero cells with 75 μg mL -1 of H. pluvialis ethanol extract inhibited virus infection by approximately 85%, whereas the same concentration of water and hexane extracts reduced the virus infectivity 75% and 50%, respectively. D. salina extracts were less effective than H. pluvialis extracts and presented a different behaviour since water and ethanol extracts produced a similar virus inhibition (65%). Moreover, H. pluvialis ethanol extract was also the most effective against HSV-1 intracellular replication. The antiviral activity of water PLE extracts was found to correlate with polysaccharides since the polysaccharide-rich fraction isolated from these extracts showed higher antiviral activity than the original water extracts. A gas chromatography-mass spectrometry (GC-MS) characterization of the H. pluvialis ethanol extract showed the antiviral activity of this extract could be partially related with the presence of short-chain fatty acids, although other compounds could be involved in this activity; meanwhile, in the case of D. salina ethanol extract other compounds seemed to be implied, such as: β-ionone, neophytadiene, phytol, palmitic acid and α-linolenic acid. The results demonstrate the use of PLE allows obtaining antiviral compounds from microalgae used as carotenoids sources, which gives the microalgae biomass an added value. © 2011 Springer Science+Business Media B.V.
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2012
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Subjects: | Antiviral activity, Microalgae, Dunaliella salina, Haematococcus pluvialis, Pressurized liquid extraction, |
Online Access: | http://hdl.handle.net/10261/101235 http://dx.doi.org/10.13039/100012818 |
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dig-cial-es-10261-1012352018-09-19T12:15:35Z Antiviral compounds obtained from microalgae commonly used as carotenoid sources Santoyo, Susana Jaime, Laura Plaza, Merichel Herrero, Miguel Rodríguez-Meizoso, Irene Ibáñez, Elena Reglero, Guillermo Comunidad de Madrid Ministerio de Educación y Ciencia (España) Antiviral activity Microalgae Dunaliella salina Haematococcus pluvialis Pressurized liquid extraction Pressurized liquid extraction (PLE), an environmentally friendly technique, has been used to obtain antiviral compounds from microalgae commonly used as carotenoid sources: Haematococcus pluvialis and Dunaliella salina. The antiviral properties of PLE extracts (hexane, ethanol and water) were evaluated against herpes simplex virus type 1 (HSV-1) at different stages during viral infection. Pretreatment of Vero cells with 75 μg mL -1 of H. pluvialis ethanol extract inhibited virus infection by approximately 85%, whereas the same concentration of water and hexane extracts reduced the virus infectivity 75% and 50%, respectively. D. salina extracts were less effective than H. pluvialis extracts and presented a different behaviour since water and ethanol extracts produced a similar virus inhibition (65%). Moreover, H. pluvialis ethanol extract was also the most effective against HSV-1 intracellular replication. The antiviral activity of water PLE extracts was found to correlate with polysaccharides since the polysaccharide-rich fraction isolated from these extracts showed higher antiviral activity than the original water extracts. A gas chromatography-mass spectrometry (GC-MS) characterization of the H. pluvialis ethanol extract showed the antiviral activity of this extract could be partially related with the presence of short-chain fatty acids, although other compounds could be involved in this activity; meanwhile, in the case of D. salina ethanol extract other compounds seemed to be implied, such as: β-ionone, neophytadiene, phytol, palmitic acid and α-linolenic acid. The results demonstrate the use of PLE allows obtaining antiviral compounds from microalgae used as carotenoids sources, which gives the microalgae biomass an added value. © 2011 Springer Science+Business Media B.V. This work has been financed by the Spanish Ministry of Education and Science (AGL2005-06726-C04), the programme CONSOLIDER-INGENIO 2010 (CDS2007-00063) and the regional programme ALIBIRD-CM S-0505/AGR-0153 from the Comunidad de Madrid, Spain. S0505/AGR-0153/ALIBIRD Peer Reviewed 2014-08-26T12:40:43Z 2014-08-26T12:40:43Z 2012 2014-08-26T12:40:43Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1007/s10811-011-9692-1 issn: 0921-8971 Journal of Applied Phycology 24(4): 731-741 (2012) http://hdl.handle.net/10261/101235 10.1007/s10811-011-9692-1 http://dx.doi.org/10.13039/100012818 Preprint http://dx.doi.org/10.1007/s10811-011-9692-1 open Springer |
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Antiviral activity Microalgae Dunaliella salina Haematococcus pluvialis Pressurized liquid extraction Antiviral activity Microalgae Dunaliella salina Haematococcus pluvialis Pressurized liquid extraction |
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Antiviral activity Microalgae Dunaliella salina Haematococcus pluvialis Pressurized liquid extraction Antiviral activity Microalgae Dunaliella salina Haematococcus pluvialis Pressurized liquid extraction Santoyo, Susana Jaime, Laura Plaza, Merichel Herrero, Miguel Rodríguez-Meizoso, Irene Ibáñez, Elena Reglero, Guillermo Antiviral compounds obtained from microalgae commonly used as carotenoid sources |
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Pressurized liquid extraction (PLE), an environmentally friendly technique, has been used to obtain antiviral compounds from microalgae commonly used as carotenoid sources: Haematococcus pluvialis and Dunaliella salina. The antiviral properties of PLE extracts (hexane, ethanol and water) were evaluated against herpes simplex virus type 1 (HSV-1) at different stages during viral infection. Pretreatment of Vero cells with 75 μg mL -1 of H. pluvialis ethanol extract inhibited virus infection by approximately 85%, whereas the same concentration of water and hexane extracts reduced the virus infectivity 75% and 50%, respectively. D. salina extracts were less effective than H. pluvialis extracts and presented a different behaviour since water and ethanol extracts produced a similar virus inhibition (65%). Moreover, H. pluvialis ethanol extract was also the most effective against HSV-1 intracellular replication. The antiviral activity of water PLE extracts was found to correlate with polysaccharides since the polysaccharide-rich fraction isolated from these extracts showed higher antiviral activity than the original water extracts. A gas chromatography-mass spectrometry (GC-MS) characterization of the H. pluvialis ethanol extract showed the antiviral activity of this extract could be partially related with the presence of short-chain fatty acids, although other compounds could be involved in this activity; meanwhile, in the case of D. salina ethanol extract other compounds seemed to be implied, such as: β-ionone, neophytadiene, phytol, palmitic acid and α-linolenic acid. The results demonstrate the use of PLE allows obtaining antiviral compounds from microalgae used as carotenoids sources, which gives the microalgae biomass an added value. © 2011 Springer Science+Business Media B.V. |
author2 |
Comunidad de Madrid |
author_facet |
Comunidad de Madrid Santoyo, Susana Jaime, Laura Plaza, Merichel Herrero, Miguel Rodríguez-Meizoso, Irene Ibáñez, Elena Reglero, Guillermo |
format |
artículo |
topic_facet |
Antiviral activity Microalgae Dunaliella salina Haematococcus pluvialis Pressurized liquid extraction |
author |
Santoyo, Susana Jaime, Laura Plaza, Merichel Herrero, Miguel Rodríguez-Meizoso, Irene Ibáñez, Elena Reglero, Guillermo |
author_sort |
Santoyo, Susana |
title |
Antiviral compounds obtained from microalgae commonly used as carotenoid sources |
title_short |
Antiviral compounds obtained from microalgae commonly used as carotenoid sources |
title_full |
Antiviral compounds obtained from microalgae commonly used as carotenoid sources |
title_fullStr |
Antiviral compounds obtained from microalgae commonly used as carotenoid sources |
title_full_unstemmed |
Antiviral compounds obtained from microalgae commonly used as carotenoid sources |
title_sort |
antiviral compounds obtained from microalgae commonly used as carotenoid sources |
publisher |
Springer |
publishDate |
2012 |
url |
http://hdl.handle.net/10261/101235 http://dx.doi.org/10.13039/100012818 |
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