Study on the 3-hydroxy-3-methyl-glutaryl CoA reductase inhibitory properties of Agaricus bisporus and extraction of bioactive fractions using pressurised solvent technologies
[Background]: Agaricus bisporus mushrooms were able to lower cholesterol levels in hypercholesterolaemic rats and it was suggested that dietary fibre might inhibit cholesterol absorption. However, A. bisporus extracts were also able to inhibit the 3-hydroxy-3-methyl-glutaryl CoA reductase (HMGCR, the key enzyme in the cholesterol biosynthetic pathway) and this might also contribute to the observed lowering of cholesterol levels in serum. [Results]: The methanol-water extracts obtained from A. bisporus were able to inhibit up to 60% the HMGCR activity using an in vitro assay. The HMGCR inhibitory capacities depended on cultivation conditions, strains, etc. The potential inhibitors were not statins, they might be β-glucans able to scavenge the substrate and impair the enzymatic reaction. They were present during all mushroom developmental stages and similarly distributed through all the tissues including the parts discarded as a by-product. Accelerated solvent extractions using 1:1 ethanol-water as pressurised solvent (10.7 MPa, 25°C, five cycles of 5 min) were more effective in the extraction of the HMGCiR inhibitor(s) than supercritical fluid extractions (9 MPa, 40°C) using CO2 with 10% ethanol. [Conclusion]: A mushroom cultivation and two extraction procedures were optimised to obtain fractions from A. bisporus with high HMGCR inhibitory activities to design novel ingredients for hypocholesterolaemic functional foodstuffs. © 2013 Society of Chemical Industry.
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Format: | artículo biblioteca |
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Blackwell Publishing
2013
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Subjects: | Supercritical fluid extraction (SFE), Cultivation, Pressurised solvent extraction (PSE), White button mushroom, HMGCoA reductase cholesterol, |
Online Access: | http://hdl.handle.net/10261/100318 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 |
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dig-cial-es-10261-1003182018-09-21T07:12:41Z Study on the 3-hydroxy-3-methyl-glutaryl CoA reductase inhibitory properties of Agaricus bisporus and extraction of bioactive fractions using pressurised solvent technologies Gil-Ramírez, Alicia Clavijo, Cristina Palanisamy, Marimuthu Ruiz-Rodríguez, Alejandro Navarro-Rubio, María Pérez, Margarita Marin, Francisco R. Reglero, Guillermo Soler-Rivas, Cristina European Commission Comunidad de Madrid Ministerio de Ciencia e Innovación (España) Gobierno de La Rioja Supercritical fluid extraction (SFE) Cultivation Pressurised solvent extraction (PSE) White button mushroom HMGCoA reductase cholesterol [Background]: Agaricus bisporus mushrooms were able to lower cholesterol levels in hypercholesterolaemic rats and it was suggested that dietary fibre might inhibit cholesterol absorption. However, A. bisporus extracts were also able to inhibit the 3-hydroxy-3-methyl-glutaryl CoA reductase (HMGCR, the key enzyme in the cholesterol biosynthetic pathway) and this might also contribute to the observed lowering of cholesterol levels in serum. [Results]: The methanol-water extracts obtained from A. bisporus were able to inhibit up to 60% the HMGCR activity using an in vitro assay. The HMGCR inhibitory capacities depended on cultivation conditions, strains, etc. The potential inhibitors were not statins, they might be β-glucans able to scavenge the substrate and impair the enzymatic reaction. They were present during all mushroom developmental stages and similarly distributed through all the tissues including the parts discarded as a by-product. Accelerated solvent extractions using 1:1 ethanol-water as pressurised solvent (10.7 MPa, 25°C, five cycles of 5 min) were more effective in the extraction of the HMGCiR inhibitor(s) than supercritical fluid extractions (9 MPa, 40°C) using CO2 with 10% ethanol. [Conclusion]: A mushroom cultivation and two extraction procedures were optimised to obtain fractions from A. bisporus with high HMGCR inhibitory activities to design novel ingredients for hypocholesterolaemic functional foodstuffs. © 2013 Society of Chemical Industry. The research was supported by the European Unionwithin the 7th framework programme (7FM-PEOPLE-2009-IIF project 251285), by the ALIBIRD-CM S2009/AGR-1469 and ADER 2010-I-ID-0093 regionalprogrammesfrom, respectively, the Community of Madrid and La Rioja (Spain), and by the AGL2010-21537 national R+D program from the Spanish Ministry of Science and Innovation. Peer Reviewed 2014-07-22T08:03:54Z 2014-07-22T08:03:54Z 2013 2014-07-22T08:03:54Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1002/jsfa.6102 issn: 0022-5142 e-issn: 1097-0010 Journal of the Science of Food and Agriculture 93(11): 2789-2796 (2013) http://hdl.handle.net/10261/100318 10.1002/jsfa.6102 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/FP7/251285 S2009/AGR-1469/ALIBIRD none Blackwell Publishing |
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Supercritical fluid extraction (SFE) Cultivation Pressurised solvent extraction (PSE) White button mushroom HMGCoA reductase cholesterol Supercritical fluid extraction (SFE) Cultivation Pressurised solvent extraction (PSE) White button mushroom HMGCoA reductase cholesterol |
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Supercritical fluid extraction (SFE) Cultivation Pressurised solvent extraction (PSE) White button mushroom HMGCoA reductase cholesterol Supercritical fluid extraction (SFE) Cultivation Pressurised solvent extraction (PSE) White button mushroom HMGCoA reductase cholesterol Gil-Ramírez, Alicia Clavijo, Cristina Palanisamy, Marimuthu Ruiz-Rodríguez, Alejandro Navarro-Rubio, María Pérez, Margarita Marin, Francisco R. Reglero, Guillermo Soler-Rivas, Cristina Study on the 3-hydroxy-3-methyl-glutaryl CoA reductase inhibitory properties of Agaricus bisporus and extraction of bioactive fractions using pressurised solvent technologies |
description |
[Background]: Agaricus bisporus mushrooms were able to lower cholesterol levels in hypercholesterolaemic rats and it was suggested that dietary fibre might inhibit cholesterol absorption. However, A. bisporus extracts were also able to inhibit the 3-hydroxy-3-methyl-glutaryl CoA reductase (HMGCR, the key enzyme in the cholesterol biosynthetic pathway) and this might also contribute to the observed lowering of cholesterol levels in serum. [Results]: The methanol-water extracts obtained from A. bisporus were able to inhibit up to 60% the HMGCR activity using an in vitro assay. The HMGCR inhibitory capacities depended on cultivation conditions, strains, etc. The potential inhibitors were not statins, they might be β-glucans able to scavenge the substrate and impair the enzymatic reaction. They were present during all mushroom developmental stages and similarly distributed through all the tissues including the parts discarded as a by-product. Accelerated solvent extractions using 1:1 ethanol-water as pressurised solvent (10.7 MPa, 25°C, five cycles of 5 min) were more effective in the extraction of the HMGCiR inhibitor(s) than supercritical fluid extractions (9 MPa, 40°C) using CO2 with 10% ethanol. [Conclusion]: A mushroom cultivation and two extraction procedures were optimised to obtain fractions from A. bisporus with high HMGCR inhibitory activities to design novel ingredients for hypocholesterolaemic functional foodstuffs. © 2013 Society of Chemical Industry. |
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European Commission |
author_facet |
European Commission Gil-Ramírez, Alicia Clavijo, Cristina Palanisamy, Marimuthu Ruiz-Rodríguez, Alejandro Navarro-Rubio, María Pérez, Margarita Marin, Francisco R. Reglero, Guillermo Soler-Rivas, Cristina |
format |
artículo |
topic_facet |
Supercritical fluid extraction (SFE) Cultivation Pressurised solvent extraction (PSE) White button mushroom HMGCoA reductase cholesterol |
author |
Gil-Ramírez, Alicia Clavijo, Cristina Palanisamy, Marimuthu Ruiz-Rodríguez, Alejandro Navarro-Rubio, María Pérez, Margarita Marin, Francisco R. Reglero, Guillermo Soler-Rivas, Cristina |
author_sort |
Gil-Ramírez, Alicia |
title |
Study on the 3-hydroxy-3-methyl-glutaryl CoA reductase inhibitory properties of Agaricus bisporus and extraction of bioactive fractions using pressurised solvent technologies |
title_short |
Study on the 3-hydroxy-3-methyl-glutaryl CoA reductase inhibitory properties of Agaricus bisporus and extraction of bioactive fractions using pressurised solvent technologies |
title_full |
Study on the 3-hydroxy-3-methyl-glutaryl CoA reductase inhibitory properties of Agaricus bisporus and extraction of bioactive fractions using pressurised solvent technologies |
title_fullStr |
Study on the 3-hydroxy-3-methyl-glutaryl CoA reductase inhibitory properties of Agaricus bisporus and extraction of bioactive fractions using pressurised solvent technologies |
title_full_unstemmed |
Study on the 3-hydroxy-3-methyl-glutaryl CoA reductase inhibitory properties of Agaricus bisporus and extraction of bioactive fractions using pressurised solvent technologies |
title_sort |
study on the 3-hydroxy-3-methyl-glutaryl coa reductase inhibitory properties of agaricus bisporus and extraction of bioactive fractions using pressurised solvent technologies |
publisher |
Blackwell Publishing |
publishDate |
2013 |
url |
http://hdl.handle.net/10261/100318 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 |
work_keys_str_mv |
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