Isolation of carsonic acid from rosemary extracts using semi-preparative supercritical fluid chromatography
Supercritical fluid chromatography (SFC) in analytical and semi-preparative scale was studied to separate rosemary (Rosmarinus officinalis) supercritical extracts to produce fractions with high antioxidant activity. Carnosic acid, the major antioxidant present in rosemary supercritical extracts, was the target compound to be isolated in the fractions produced. First, rosemary extracts were produced in a supercritical CO2 pilot-plant employing different extraction conditions, in order to obtain samples with diverse composition. The content of carnosic acid was determined and the DPPH test was accomplished in order to assess the antioxidant activity of the different samples produced. Then, using some of these samples, experiments were carried out in a semi-preparative SFC system testing the performance of four different chromatographic columns to isolate and recover a fraction with high concentration of carnosic acid. The four chromatographic columns employed were silica and 2-ethylpyridine (especially packed for SFC) and silica and diol columns (packed for HPLC). The SFC fractionation conditions explored were in the ranges 10-20MPa, 313-333K and 5-20% of cosolvent (ethanol). Using the first three columns, it was possible to isolate fractions with concentrations of carnosic acid greater than 80% mass. © 2013 Elsevier B.V.
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Elsevier
2013
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Subjects: | Supercritical carbon dioxide, Supercritical chromatography, Carnosic acid, Antioxidants, Rosemary, |
Online Access: | http://hdl.handle.net/10261/100125 http://dx.doi.org/10.13039/501100004837 |
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dig-cial-es-10261-1001252019-10-14T07:23:06Z Isolation of carsonic acid from rosemary extracts using semi-preparative supercritical fluid chromatography Vicente, G. García-Risco, Mónica R. Fornari, Tiziana Reglero, Guillermo Ministerio de Ciencia e Innovación (España) Supercritical carbon dioxide Supercritical chromatography Carnosic acid Antioxidants Rosemary Supercritical fluid chromatography (SFC) in analytical and semi-preparative scale was studied to separate rosemary (Rosmarinus officinalis) supercritical extracts to produce fractions with high antioxidant activity. Carnosic acid, the major antioxidant present in rosemary supercritical extracts, was the target compound to be isolated in the fractions produced. First, rosemary extracts were produced in a supercritical CO2 pilot-plant employing different extraction conditions, in order to obtain samples with diverse composition. The content of carnosic acid was determined and the DPPH test was accomplished in order to assess the antioxidant activity of the different samples produced. Then, using some of these samples, experiments were carried out in a semi-preparative SFC system testing the performance of four different chromatographic columns to isolate and recover a fraction with high concentration of carnosic acid. The four chromatographic columns employed were silica and 2-ethylpyridine (especially packed for SFC) and silica and diol columns (packed for HPLC). The SFC fractionation conditions explored were in the ranges 10-20MPa, 313-333K and 5-20% of cosolvent (ethanol). Using the first three columns, it was possible to isolate fractions with concentrations of carnosic acid greater than 80% mass. © 2013 Elsevier B.V. This work has been financed by project AGL2010-21565 (subprogram ALI) and project INNSAMED IPT-300000-2010-34 (subprogram INNPACTO) from Ministerio de Ciencia e Innovación (Spain). Peer Reviewed 2014-07-18T07:53:21Z 2014-07-18T07:53:21Z 2013 2014-07-18T07:53:21Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.chroma.2013.02.044 issn: 0021-9673 e-issn: 1873-3778 Journal of Chromatography A 1286: 208-215 (2013) http://hdl.handle.net/10261/100125 10.1016/j.chroma.2013.02.044 http://dx.doi.org/10.13039/501100004837 Preprint http://dx.doi.org/10.1016/j.chroma.2013.02.044 open Elsevier |
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Supercritical carbon dioxide Supercritical chromatography Carnosic acid Antioxidants Rosemary Supercritical carbon dioxide Supercritical chromatography Carnosic acid Antioxidants Rosemary |
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Supercritical carbon dioxide Supercritical chromatography Carnosic acid Antioxidants Rosemary Supercritical carbon dioxide Supercritical chromatography Carnosic acid Antioxidants Rosemary Vicente, G. García-Risco, Mónica R. Fornari, Tiziana Reglero, Guillermo Isolation of carsonic acid from rosemary extracts using semi-preparative supercritical fluid chromatography |
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Supercritical fluid chromatography (SFC) in analytical and semi-preparative scale was studied to separate rosemary (Rosmarinus officinalis) supercritical extracts to produce fractions with high antioxidant activity. Carnosic acid, the major antioxidant present in rosemary supercritical extracts, was the target compound to be isolated in the fractions produced. First, rosemary extracts were produced in a supercritical CO2 pilot-plant employing different extraction conditions, in order to obtain samples with diverse composition. The content of carnosic acid was determined and the DPPH test was accomplished in order to assess the antioxidant activity of the different samples produced. Then, using some of these samples, experiments were carried out in a semi-preparative SFC system testing the performance of four different chromatographic columns to isolate and recover a fraction with high concentration of carnosic acid. The four chromatographic columns employed were silica and 2-ethylpyridine (especially packed for SFC) and silica and diol columns (packed for HPLC). The SFC fractionation conditions explored were in the ranges 10-20MPa, 313-333K and 5-20% of cosolvent (ethanol). Using the first three columns, it was possible to isolate fractions with concentrations of carnosic acid greater than 80% mass. © 2013 Elsevier B.V. |
author2 |
Ministerio de Ciencia e Innovación (España) |
author_facet |
Ministerio de Ciencia e Innovación (España) Vicente, G. García-Risco, Mónica R. Fornari, Tiziana Reglero, Guillermo |
format |
artículo |
topic_facet |
Supercritical carbon dioxide Supercritical chromatography Carnosic acid Antioxidants Rosemary |
author |
Vicente, G. García-Risco, Mónica R. Fornari, Tiziana Reglero, Guillermo |
author_sort |
Vicente, G. |
title |
Isolation of carsonic acid from rosemary extracts using semi-preparative supercritical fluid chromatography |
title_short |
Isolation of carsonic acid from rosemary extracts using semi-preparative supercritical fluid chromatography |
title_full |
Isolation of carsonic acid from rosemary extracts using semi-preparative supercritical fluid chromatography |
title_fullStr |
Isolation of carsonic acid from rosemary extracts using semi-preparative supercritical fluid chromatography |
title_full_unstemmed |
Isolation of carsonic acid from rosemary extracts using semi-preparative supercritical fluid chromatography |
title_sort |
isolation of carsonic acid from rosemary extracts using semi-preparative supercritical fluid chromatography |
publisher |
Elsevier |
publishDate |
2013 |
url |
http://hdl.handle.net/10261/100125 http://dx.doi.org/10.13039/501100004837 |
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