Identification of white spot syndrome virus in cultured Penaeus indicus by polymerase chain reaction (PCR) in I.R. IRAN
The WSD is the important viral shrimp disease in past decade. The detection of virus in each country was investigated by polymerase chain reaction for sensitivity and accurate. In this research study we collected 23 samples from shrimp suspected to WSD. The DNA from samples collected and extracted and design two kind of primer from VP24 identified in gene bank by DNAsis software. A primer also designs for Housekeeping gene for positive and negative samples in all examined. The results showed the gene colon for wssv is the similar with others and 97% is consistency. The product of PCR was colon in plasmid and confirmed and this plasmid used for internal control.
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| Main Authors: | , , , , , , |
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| Format: | monograph biblioteca |
| Language: | Persian |
| Published: |
Iranian Fisheries Science Research Institute
2007
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| Subjects: | Health, Iran, White spot syndrome virus, DNA, Polymerase chain reaction, WSD, Viral, Shrimp, Samples, Penaeus indicus, Polymerase, WSSV, Virus, |
| Online Access: | http://hdl.handle.net/1834/39520 |
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