Assessment of genetic diversity of the Black Sea turbot Scophthalmus maeoticus population
In the Russian Federation, the Black Sea turbot Scophthalmus maeoticus (Pallas, 1814) is one of the most important aquatic species for aquaculture; it is promising for domestic practice of both artificial reproduction and commercial aquaculture. In the near future, the Black Sea and Azov populations of the Black Sea turbot will be used to form the broodstocks and obtain juveniles. For this purpose, eight loci of the Black Sea turbot Scophthalmus maeoticus, which have been tested on three samples (Crimean fishing area of the Black Sea, Caucasian fishing area of the Black Sea, the Sea of Azov), were analyzed. Research methods include sampling, DNA isolation by means of spin-column method, PCR amplification, subsequent analysis of the obtained PCR product in the genetic analyzer Nanofor-05, and statistical processing of the data using FreeNa and GenAlEx software. As a result of the analysis, the value of average expected heterozygosity, the number of private alleles, variability of the investigated loci, deviation from the Hardy-Weinberg equilibrium, as well as the level of genetic differentiation, and the percentage of migrants per generation between the three studied samples have been determined.
| Main Authors: | , |
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| Other Authors: | |
| Format: | Journal Contribution biblioteca |
| Language: | Russian |
| Published: |
2019
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| Subjects: | Scophthalmus maeoticus, Black Sea turbot, Commercial aquaculture, Artificial reproduction, DNA, Alleles, Brood stock, Juveniles, Genetic differentiation, Черноморский калкан, ДНК, Товарная аквакультура, Молодь, Маточное стадо, Искусственное воспроизводство, |
| Online Access: | http://hdl.handle.net/1834/16136 |
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