Identification of white spot syndrom virus in cultured Penaeus indicus by polymerase Chain reaction (PCR) in I.R. IRAN

The WSD is the important viral shrimp disease in past decade. The detection of virus in each country was investigated by polymerase chain reaction for sensitivity and accurate. In this research study we collected 23 samples from shrimp suspected to WSD. The DNA from samples collected and extracted and design two kind of primer from VP24 identified in gene bank by DNAsis software. A primer also designs for House keeping gene for positive and negative samples in all examined. The results showed the gene colon for wssv is the similar with others and 97% is consistency. The product of PCR was colon in plasmid and confirmed and this plasmid used for internal control.

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Bibliographic Details
Main Authors: Saberi, Amir Masoud, Afsharnasab, M., Ghoroghi, A., Laloui, F., Bandehpour, M., Tamjidi, B., Negarsid, H.
Format: Report biblioteca
Language:Persian
Published: Iranian Fisheries Science Research Institute 2007
Subjects:White spot syndrome virus, DNA, Polymerase chain reaction, WSD, Viral, Shrimp, Samples, Penaeus indicus, Polymerase, WSSV, Virus,
Online Access:http://hdl.handle.net/1834/13077
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spelling dig-aquadocs-1834-130772021-05-19T06:50:08Z Identification of white spot syndrom virus in cultured Penaeus indicus by polymerase Chain reaction (PCR) in I.R. IRAN Saberi, Amir Masoud Afsharnasab, M. Ghoroghi, A. Laloui, F. Bandehpour, M. Tamjidi, B. Negarsid, H. White spot syndrome virus DNA Polymerase chain reaction WSD Viral Shrimp Samples Penaeus indicus Polymerase WSSV Virus The WSD is the important viral shrimp disease in past decade. The detection of virus in each country was investigated by polymerase chain reaction for sensitivity and accurate. In this research study we collected 23 samples from shrimp suspected to WSD. The DNA from samples collected and extracted and design two kind of primer from VP24 identified in gene bank by DNAsis software. A primer also designs for House keeping gene for positive and negative samples in all examined. The results showed the gene colon for wssv is the similar with others and 97% is consistency. The product of PCR was colon in plasmid and confirmed and this plasmid used for internal control. Iranian Fisheries Science Research Institute Published 2018-07-01T12:54:58Z 2018-07-01T12:54:58Z 2007 Report Refereed http://hdl.handle.net/1834/13077 fa 86.842; 39pp. Iran Iranian Fisheries Science Research Institute Tehran, Iran
institution UNESCO
collection DSpace
country Francia
countrycode FR
component Bibliográfico
access En linea
databasecode dig-aquadocs
tag biblioteca
region Europa del Oeste
libraryname Repositorio AQUADOCS
language Persian
topic White spot syndrome virus
DNA
Polymerase chain reaction
WSD
Viral
Shrimp
Samples
Penaeus indicus
Polymerase
WSSV
Virus
White spot syndrome virus
DNA
Polymerase chain reaction
WSD
Viral
Shrimp
Samples
Penaeus indicus
Polymerase
WSSV
Virus
spellingShingle White spot syndrome virus
DNA
Polymerase chain reaction
WSD
Viral
Shrimp
Samples
Penaeus indicus
Polymerase
WSSV
Virus
White spot syndrome virus
DNA
Polymerase chain reaction
WSD
Viral
Shrimp
Samples
Penaeus indicus
Polymerase
WSSV
Virus
Saberi, Amir Masoud
Afsharnasab, M.
Ghoroghi, A.
Laloui, F.
Bandehpour, M.
Tamjidi, B.
Negarsid, H.
Identification of white spot syndrom virus in cultured Penaeus indicus by polymerase Chain reaction (PCR) in I.R. IRAN
description The WSD is the important viral shrimp disease in past decade. The detection of virus in each country was investigated by polymerase chain reaction for sensitivity and accurate. In this research study we collected 23 samples from shrimp suspected to WSD. The DNA from samples collected and extracted and design two kind of primer from VP24 identified in gene bank by DNAsis software. A primer also designs for House keeping gene for positive and negative samples in all examined. The results showed the gene colon for wssv is the similar with others and 97% is consistency. The product of PCR was colon in plasmid and confirmed and this plasmid used for internal control.
format Report
topic_facet White spot syndrome virus
DNA
Polymerase chain reaction
WSD
Viral
Shrimp
Samples
Penaeus indicus
Polymerase
WSSV
Virus
author Saberi, Amir Masoud
Afsharnasab, M.
Ghoroghi, A.
Laloui, F.
Bandehpour, M.
Tamjidi, B.
Negarsid, H.
author_facet Saberi, Amir Masoud
Afsharnasab, M.
Ghoroghi, A.
Laloui, F.
Bandehpour, M.
Tamjidi, B.
Negarsid, H.
author_sort Saberi, Amir Masoud
title Identification of white spot syndrom virus in cultured Penaeus indicus by polymerase Chain reaction (PCR) in I.R. IRAN
title_short Identification of white spot syndrom virus in cultured Penaeus indicus by polymerase Chain reaction (PCR) in I.R. IRAN
title_full Identification of white spot syndrom virus in cultured Penaeus indicus by polymerase Chain reaction (PCR) in I.R. IRAN
title_fullStr Identification of white spot syndrom virus in cultured Penaeus indicus by polymerase Chain reaction (PCR) in I.R. IRAN
title_full_unstemmed Identification of white spot syndrom virus in cultured Penaeus indicus by polymerase Chain reaction (PCR) in I.R. IRAN
title_sort identification of white spot syndrom virus in cultured penaeus indicus by polymerase chain reaction (pcr) in i.r. iran
publisher Iranian Fisheries Science Research Institute
publishDate 2007
url http://hdl.handle.net/1834/13077
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