Identification of white spot syndrom virus in cultured Penaeus indicus by polymerase Chain reaction (PCR) in I.R. IRAN
The WSD is the important viral shrimp disease in past decade. The detection of virus in each country was investigated by polymerase chain reaction for sensitivity and accurate. In this research study we collected 23 samples from shrimp suspected to WSD. The DNA from samples collected and extracted and design two kind of primer from VP24 identified in gene bank by DNAsis software. A primer also designs for House keeping gene for positive and negative samples in all examined. The results showed the gene colon for wssv is the similar with others and 97% is consistency. The product of PCR was colon in plasmid and confirmed and this plasmid used for internal control.
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Format: | Report biblioteca |
Language: | Persian |
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Iranian Fisheries Science Research Institute
2007
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Subjects: | White spot syndrome virus, DNA, Polymerase chain reaction, WSD, Viral, Shrimp, Samples, Penaeus indicus, Polymerase, WSSV, Virus, |
Online Access: | http://hdl.handle.net/1834/13077 |
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dig-aquadocs-1834-130772021-05-19T06:50:08Z Identification of white spot syndrom virus in cultured Penaeus indicus by polymerase Chain reaction (PCR) in I.R. IRAN Saberi, Amir Masoud Afsharnasab, M. Ghoroghi, A. Laloui, F. Bandehpour, M. Tamjidi, B. Negarsid, H. White spot syndrome virus DNA Polymerase chain reaction WSD Viral Shrimp Samples Penaeus indicus Polymerase WSSV Virus The WSD is the important viral shrimp disease in past decade. The detection of virus in each country was investigated by polymerase chain reaction for sensitivity and accurate. In this research study we collected 23 samples from shrimp suspected to WSD. The DNA from samples collected and extracted and design two kind of primer from VP24 identified in gene bank by DNAsis software. A primer also designs for House keeping gene for positive and negative samples in all examined. The results showed the gene colon for wssv is the similar with others and 97% is consistency. The product of PCR was colon in plasmid and confirmed and this plasmid used for internal control. Iranian Fisheries Science Research Institute Published 2018-07-01T12:54:58Z 2018-07-01T12:54:58Z 2007 Report Refereed http://hdl.handle.net/1834/13077 fa 86.842; 39pp. Iran Iranian Fisheries Science Research Institute Tehran, Iran |
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White spot syndrome virus DNA Polymerase chain reaction WSD Viral Shrimp Samples Penaeus indicus Polymerase WSSV Virus White spot syndrome virus DNA Polymerase chain reaction WSD Viral Shrimp Samples Penaeus indicus Polymerase WSSV Virus |
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White spot syndrome virus DNA Polymerase chain reaction WSD Viral Shrimp Samples Penaeus indicus Polymerase WSSV Virus White spot syndrome virus DNA Polymerase chain reaction WSD Viral Shrimp Samples Penaeus indicus Polymerase WSSV Virus Saberi, Amir Masoud Afsharnasab, M. Ghoroghi, A. Laloui, F. Bandehpour, M. Tamjidi, B. Negarsid, H. Identification of white spot syndrom virus in cultured Penaeus indicus by polymerase Chain reaction (PCR) in I.R. IRAN |
description |
The WSD is the important viral shrimp disease in past decade. The detection of virus in each country was investigated by polymerase chain reaction for sensitivity and accurate. In this research study we collected 23 samples from shrimp suspected to WSD.
The DNA from samples collected and extracted and design two kind of primer from VP24 identified in gene bank by DNAsis software. A primer also designs for House keeping gene for positive and negative samples in all examined. The results showed the gene colon for wssv is the similar with others and 97% is consistency.
The product of PCR was colon in plasmid and confirmed and this plasmid used for internal control. |
format |
Report |
topic_facet |
White spot syndrome virus DNA Polymerase chain reaction WSD Viral Shrimp Samples Penaeus indicus Polymerase WSSV Virus |
author |
Saberi, Amir Masoud Afsharnasab, M. Ghoroghi, A. Laloui, F. Bandehpour, M. Tamjidi, B. Negarsid, H. |
author_facet |
Saberi, Amir Masoud Afsharnasab, M. Ghoroghi, A. Laloui, F. Bandehpour, M. Tamjidi, B. Negarsid, H. |
author_sort |
Saberi, Amir Masoud |
title |
Identification of white spot syndrom virus in cultured Penaeus indicus by polymerase Chain reaction (PCR) in I.R. IRAN |
title_short |
Identification of white spot syndrom virus in cultured Penaeus indicus by polymerase Chain reaction (PCR) in I.R. IRAN |
title_full |
Identification of white spot syndrom virus in cultured Penaeus indicus by polymerase Chain reaction (PCR) in I.R. IRAN |
title_fullStr |
Identification of white spot syndrom virus in cultured Penaeus indicus by polymerase Chain reaction (PCR) in I.R. IRAN |
title_full_unstemmed |
Identification of white spot syndrom virus in cultured Penaeus indicus by polymerase Chain reaction (PCR) in I.R. IRAN |
title_sort |
identification of white spot syndrom virus in cultured penaeus indicus by polymerase chain reaction (pcr) in i.r. iran |
publisher |
Iranian Fisheries Science Research Institute |
publishDate |
2007 |
url |
http://hdl.handle.net/1834/13077 |
work_keys_str_mv |
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