Karyological analysis of Cyprinion macrostomum Heckel, 1843, from Godarkhosh River, Ilam Province, Iran
In this study, for the first time in Iran, the karyotype of bigmouth Lotak, Cyprinion macrostomum Heckel, 1843, was investigated through examining metaphase chromosomes of seven fish with mean weight 30±5g caught by electrofishing from Godarkhosh River in Ilam Province. To stimulate cell divisions, fish were injected intraperitoneally two times by phytohemagglutinin (PHA). The cell divisions were arrested in metaphase stage by intraperitoneal injection of colchicine. Well-separated cells were obtained from kidney and gill filament and chromosome spreads were prepared and stained with giemsa. Karyotype was obtained as 2n=50. The karyotype consisted of 5 metacentric, 12 submetacentric and 8 telocentric chromosome pairs. Centromeric index, arm ratio and Fundamental Number (FN) were determined as 0-50, 1-∞, and 84, respectively.
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Format: | Journal Contribution biblioteca |
Language: | English |
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2015
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Subjects: | Biology, Physiology, Bigmouth lotak, Cyprinion macrostomum, Karyotype, |
Online Access: | http://hdl.handle.net/1834/11907 |
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dig-aquadocs-1834-119072021-05-19T06:42:42Z Karyological analysis of Cyprinion macrostomum Heckel, 1843, from Godarkhosh River, Ilam Province, Iran Nasri, M. Keivany, Y. Dorafshan, S. Biology Physiology Bigmouth lotak Cyprinion macrostomum Karyotype In this study, for the first time in Iran, the karyotype of bigmouth Lotak, Cyprinion macrostomum Heckel, 1843, was investigated through examining metaphase chromosomes of seven fish with mean weight 30±5g caught by electrofishing from Godarkhosh River in Ilam Province. To stimulate cell divisions, fish were injected intraperitoneally two times by phytohemagglutinin (PHA). The cell divisions were arrested in metaphase stage by intraperitoneal injection of colchicine. Well-separated cells were obtained from kidney and gill filament and chromosome spreads were prepared and stained with giemsa. Karyotype was obtained as 2n=50. The karyotype consisted of 5 metacentric, 12 submetacentric and 8 telocentric chromosome pairs. Centromeric index, arm ratio and Fundamental Number (FN) were determined as 0-50, 1-∞, and 84, respectively. Published 2018-03-02T12:54:13Z 2018-03-02T12:54:13Z 2015 Journal Contribution Refereed 1562-2916 http://hdl.handle.net/1834/11907 en pp.786-796 Iran Ilam provience Godarkhosh River |
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Biology Physiology Bigmouth lotak Cyprinion macrostomum Karyotype Biology Physiology Bigmouth lotak Cyprinion macrostomum Karyotype |
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Biology Physiology Bigmouth lotak Cyprinion macrostomum Karyotype Biology Physiology Bigmouth lotak Cyprinion macrostomum Karyotype Nasri, M. Keivany, Y. Dorafshan, S. Karyological analysis of Cyprinion macrostomum Heckel, 1843, from Godarkhosh River, Ilam Province, Iran |
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In this study, for the first time in Iran, the karyotype of bigmouth Lotak, Cyprinion macrostomum Heckel, 1843, was investigated through examining metaphase chromosomes of seven fish with mean weight 30±5g caught by electrofishing from Godarkhosh River in Ilam Province. To stimulate cell divisions, fish were injected intraperitoneally two times by phytohemagglutinin (PHA). The cell divisions were arrested in metaphase stage by intraperitoneal injection of colchicine. Well-separated cells were obtained from kidney and gill filament and chromosome spreads were prepared and stained with giemsa. Karyotype was obtained as 2n=50. The karyotype consisted of 5 metacentric, 12 submetacentric and 8 telocentric chromosome pairs. Centromeric index, arm ratio and Fundamental Number (FN) were determined as 0-50, 1-∞, and 84, respectively. |
format |
Journal Contribution |
topic_facet |
Biology Physiology Bigmouth lotak Cyprinion macrostomum Karyotype |
author |
Nasri, M. Keivany, Y. Dorafshan, S. |
author_facet |
Nasri, M. Keivany, Y. Dorafshan, S. |
author_sort |
Nasri, M. |
title |
Karyological analysis of Cyprinion macrostomum Heckel, 1843, from Godarkhosh River, Ilam Province, Iran |
title_short |
Karyological analysis of Cyprinion macrostomum Heckel, 1843, from Godarkhosh River, Ilam Province, Iran |
title_full |
Karyological analysis of Cyprinion macrostomum Heckel, 1843, from Godarkhosh River, Ilam Province, Iran |
title_fullStr |
Karyological analysis of Cyprinion macrostomum Heckel, 1843, from Godarkhosh River, Ilam Province, Iran |
title_full_unstemmed |
Karyological analysis of Cyprinion macrostomum Heckel, 1843, from Godarkhosh River, Ilam Province, Iran |
title_sort |
karyological analysis of cyprinion macrostomum heckel, 1843, from godarkhosh river, ilam province, iran |
publishDate |
2015 |
url |
http://hdl.handle.net/1834/11907 |
work_keys_str_mv |
AT nasrim karyologicalanalysisofcyprinionmacrostomumheckel1843fromgodarkhoshriverilamprovinceiran AT keivanyy karyologicalanalysisofcyprinionmacrostomumheckel1843fromgodarkhoshriverilamprovinceiran AT dorafshans karyologicalanalysisofcyprinionmacrostomumheckel1843fromgodarkhoshriverilamprovinceiran |
_version_ |
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