A new method for venom extraction from venomous fish, Green Scat
Scatophagus argus argus (Green Scat) is a pretty aquarium fish. Its hard spines are venomous and can cause painful injury. In this study 60 specimens of Green Scat were collected periodically from coastal waters of Boushehr (south of Iran) from May 2011 to April 2012. Anatomical features of venomous spines were investigated. Scat venom was extracted from the spines in a new manner for keeping the specimens alive. The nature of venom was tested by SDS-PAGE. Ethical issues and animal welfare principles such as rapid and instantaneous anesthetizing, post operation disinfection and fast recovery of the specimens was practiced in order to minimize the complications. This method enhanced the purity and quantity of venom as demonstrated by 12 separated proteins in electrophoresis. New ethical issues were developed to surviving the specimens and prolong viability as well.
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Format: | Journal Contribution biblioteca |
Language: | English |
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2015
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Subjects: | Toxicology, Scatophagus argus argus, Venomous spine, Venom extraction, Viability, Ethical issues, |
Online Access: | http://hdl.handle.net/1834/11840 |
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dig-aquadocs-1834-118402021-05-19T06:42:58Z A new method for venom extraction from venomous fish, Green Scat Ghafari Khalaf Mohamadi, S.M. Shahbazzadeh, D. Jamili, Sh. Fatemi, S.M.R. Pooshang Bagheri, K. Toxicology Scatophagus argus argus Venomous spine Venom extraction Viability Ethical issues Scatophagus argus argus (Green Scat) is a pretty aquarium fish. Its hard spines are venomous and can cause painful injury. In this study 60 specimens of Green Scat were collected periodically from coastal waters of Boushehr (south of Iran) from May 2011 to April 2012. Anatomical features of venomous spines were investigated. Scat venom was extracted from the spines in a new manner for keeping the specimens alive. The nature of venom was tested by SDS-PAGE. Ethical issues and animal welfare principles such as rapid and instantaneous anesthetizing, post operation disinfection and fast recovery of the specimens was practiced in order to minimize the complications. This method enhanced the purity and quantity of venom as demonstrated by 12 separated proteins in electrophoresis. New ethical issues were developed to surviving the specimens and prolong viability as well. Published 2018-03-01T11:32:31Z 2018-03-01T11:32:31Z 2015 Journal Contribution Refereed 1562-2916 http://hdl.handle.net/1834/11840 en pp.321-327 Iran Bushehr |
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Toxicology Scatophagus argus argus Venomous spine Venom extraction Viability Ethical issues Toxicology Scatophagus argus argus Venomous spine Venom extraction Viability Ethical issues |
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Toxicology Scatophagus argus argus Venomous spine Venom extraction Viability Ethical issues Toxicology Scatophagus argus argus Venomous spine Venom extraction Viability Ethical issues Ghafari Khalaf Mohamadi, S.M. Shahbazzadeh, D. Jamili, Sh. Fatemi, S.M.R. Pooshang Bagheri, K. A new method for venom extraction from venomous fish, Green Scat |
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Scatophagus argus argus (Green Scat) is a pretty aquarium fish. Its hard spines are venomous and can cause painful injury. In this study 60 specimens of Green Scat were collected periodically from coastal waters of Boushehr (south of Iran) from May 2011 to April 2012. Anatomical features of venomous spines were investigated. Scat venom was extracted from the spines in a new manner for keeping the specimens alive. The nature of venom was tested by SDS-PAGE. Ethical issues and animal welfare principles such as rapid and instantaneous anesthetizing, post operation disinfection and fast recovery of the specimens was practiced in order to minimize the complications. This method enhanced the purity and quantity of venom as demonstrated by 12 separated proteins in electrophoresis. New ethical issues were developed to surviving the specimens and prolong viability as well. |
format |
Journal Contribution |
topic_facet |
Toxicology Scatophagus argus argus Venomous spine Venom extraction Viability Ethical issues |
author |
Ghafari Khalaf Mohamadi, S.M. Shahbazzadeh, D. Jamili, Sh. Fatemi, S.M.R. Pooshang Bagheri, K. |
author_facet |
Ghafari Khalaf Mohamadi, S.M. Shahbazzadeh, D. Jamili, Sh. Fatemi, S.M.R. Pooshang Bagheri, K. |
author_sort |
Ghafari Khalaf Mohamadi, S.M. |
title |
A new method for venom extraction from venomous fish, Green Scat |
title_short |
A new method for venom extraction from venomous fish, Green Scat |
title_full |
A new method for venom extraction from venomous fish, Green Scat |
title_fullStr |
A new method for venom extraction from venomous fish, Green Scat |
title_full_unstemmed |
A new method for venom extraction from venomous fish, Green Scat |
title_sort |
new method for venom extraction from venomous fish, green scat |
publishDate |
2015 |
url |
http://hdl.handle.net/1834/11840 |
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