Feijoeiro (Phaseolus vulgaris L.) transgênico contendo um mutante do gene REP apresenta resistência ao vírus do mosaico dourado.
Tbe rep gene of Bean golden mosaic virus (BGMV) is essential for virus replication, and rep gene rnutations with amino acid codons cbanges in the putative NTP-binding rnotif D262R (MI) was created. Phaseolus vulgaris rransforrnation was achieved with a helium driven particle acceleration device. Tbe vector contained the bar gene [resistance to arnmonium glyphosinate (AG)] and besides the rep gene mutant. A total of J 7 initial - R(O) - transformants were analyzed. From the R(O) plant M1/4, which had mutation MI, six progeny plants with resistance to AG were obtained, and four remained BGMV symptomless after inoculation. From tbese four plants, a total progeny of 127 R(2) plants were inoculated. Of the 35 progenies of plant M 1/4/l, only three plants showed syrnptoms. From the other 92 plants, 72 had symptoms. Two plants out of 20 in the nontransgenic control plants remained symptornless.
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Format: | Parte de livro biblioteca |
Language: | pt_BR pt_BR |
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2001-12-26
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Subjects: | BGMV, Tolerância, Biobalística, Transformação, Feijão, Mosaico Dourado, Phaseolus Vulgaris, Vírus, Bean golden mosaic virus, mutation, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/209188 |
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dig-alice-doc-2091882020-01-14T18:09:03Z Feijoeiro (Phaseolus vulgaris L.) transgênico contendo um mutante do gene REP apresenta resistência ao vírus do mosaico dourado. FARIA, J. C. ALBINO, M. M. C. DIAS, B. B. A. VIANNA, M. G. R. ARAGÃO, F. J. L. JOSIAS CORREA DE FARIA, CNPAF; FRANCISCO JOSE LIMA ARAGAO, Cenargen. BGMV Tolerância Biobalística Transformação Feijão Mosaico Dourado Phaseolus Vulgaris Vírus Bean golden mosaic virus mutation Tbe rep gene of Bean golden mosaic virus (BGMV) is essential for virus replication, and rep gene rnutations with amino acid codons cbanges in the putative NTP-binding rnotif D262R (MI) was created. Phaseolus vulgaris rransforrnation was achieved with a helium driven particle acceleration device. Tbe vector contained the bar gene [resistance to arnmonium glyphosinate (AG)] and besides the rep gene mutant. A total of J 7 initial - R(O) - transformants were analyzed. From the R(O) plant M1/4, which had mutation MI, six progeny plants with resistance to AG were obtained, and four remained BGMV symptomless after inoculation. From tbese four plants, a total progeny of 127 R(2) plants were inoculated. Of the 35 progenies of plant M 1/4/l, only three plants showed syrnptoms. From the other 92 plants, 72 had symptoms. Two plants out of 20 in the nontransgenic control plants remained symptornless. 2020-01-14T18:08:57Z 2020-01-14T18:08:57Z 2001-12-26 2001 2020-01-14T18:08:57Z Parte de livro In: CONGRESSO DA SOCIEDADE BRASILEIRA DE BIOTECNOLOGIA, 1., 2001, São Paulo. [Resumos expandidos]... Brasilia: SBBIOTEC, 2001. http://www.alice.cnptia.embrapa.br/alice/handle/doc/209188 pt_BR pt_BR openAccess |
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BGMV Tolerância Biobalística Transformação Feijão Mosaico Dourado Phaseolus Vulgaris Vírus Bean golden mosaic virus mutation BGMV Tolerância Biobalística Transformação Feijão Mosaico Dourado Phaseolus Vulgaris Vírus Bean golden mosaic virus mutation |
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BGMV Tolerância Biobalística Transformação Feijão Mosaico Dourado Phaseolus Vulgaris Vírus Bean golden mosaic virus mutation BGMV Tolerância Biobalística Transformação Feijão Mosaico Dourado Phaseolus Vulgaris Vírus Bean golden mosaic virus mutation FARIA, J. C. ALBINO, M. M. C. DIAS, B. B. A. VIANNA, M. G. R. ARAGÃO, F. J. L. Feijoeiro (Phaseolus vulgaris L.) transgênico contendo um mutante do gene REP apresenta resistência ao vírus do mosaico dourado. |
description |
Tbe rep gene of Bean golden mosaic virus (BGMV) is essential for virus replication, and rep gene rnutations with amino acid codons cbanges in the putative NTP-binding rnotif D262R (MI) was created. Phaseolus vulgaris rransforrnation was achieved with a helium driven particle acceleration device. Tbe vector contained the bar gene [resistance to arnmonium glyphosinate (AG)] and besides the rep gene mutant. A total of J 7 initial - R(O) - transformants were analyzed. From the R(O) plant M1/4, which had mutation MI, six progeny plants with resistance to AG were obtained, and four remained BGMV symptomless after inoculation. From tbese four plants, a total progeny of 127 R(2) plants were inoculated. Of the 35 progenies of plant M 1/4/l, only three plants showed syrnptoms. From the other 92 plants, 72 had symptoms. Two plants out of 20 in the nontransgenic control plants remained symptornless. |
author2 |
JOSIAS CORREA DE FARIA, CNPAF; FRANCISCO JOSE LIMA ARAGAO, Cenargen. |
author_facet |
JOSIAS CORREA DE FARIA, CNPAF; FRANCISCO JOSE LIMA ARAGAO, Cenargen. FARIA, J. C. ALBINO, M. M. C. DIAS, B. B. A. VIANNA, M. G. R. ARAGÃO, F. J. L. |
format |
Parte de livro |
topic_facet |
BGMV Tolerância Biobalística Transformação Feijão Mosaico Dourado Phaseolus Vulgaris Vírus Bean golden mosaic virus mutation |
author |
FARIA, J. C. ALBINO, M. M. C. DIAS, B. B. A. VIANNA, M. G. R. ARAGÃO, F. J. L. |
author_sort |
FARIA, J. C. |
title |
Feijoeiro (Phaseolus vulgaris L.) transgênico contendo um mutante do gene REP apresenta resistência ao vírus do mosaico dourado. |
title_short |
Feijoeiro (Phaseolus vulgaris L.) transgênico contendo um mutante do gene REP apresenta resistência ao vírus do mosaico dourado. |
title_full |
Feijoeiro (Phaseolus vulgaris L.) transgênico contendo um mutante do gene REP apresenta resistência ao vírus do mosaico dourado. |
title_fullStr |
Feijoeiro (Phaseolus vulgaris L.) transgênico contendo um mutante do gene REP apresenta resistência ao vírus do mosaico dourado. |
title_full_unstemmed |
Feijoeiro (Phaseolus vulgaris L.) transgênico contendo um mutante do gene REP apresenta resistência ao vírus do mosaico dourado. |
title_sort |
feijoeiro (phaseolus vulgaris l.) transgênico contendo um mutante do gene rep apresenta resistência ao vírus do mosaico dourado. |
publishDate |
2001-12-26 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/209188 |
work_keys_str_mv |
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