Changes recorded in cardiac graphoelements bees (Apis mellifera) during contact with fipronil.

A mortality of bees (Apis mellifera) caused by fipronil intoxication, due to its indiscriminate use in crops, has long been attracting the scientific community’s attention, either due to its acute or residual effects. In this study, we assessed the cardiac activity as a biomarker of fipronil intoxication through electrophysiological recordings of bees. Eighteen foragers, from the apiary of EMBRAPA—Eastern Amazon (Belém-Pará), bees were previously anesthetized at low temperature (− 10 °C) for 5 min and properly restrained and fixed on a stereotaxic base, where electrodes were implanted. All these procedures were carried out within a Faraday cage. Eighteen bees were used in the study. Worker bees engaged in foraging activities were selected. The bees were divided into a control group and a group treated with fipronil at 0.025 mg/bee (n = 9). The recordings lasted for 4 min and were evaluated at 1-s intervals represented by the following letters: A = (2–3 s), B = (59–60 s), C = (119–120 s), D = (179–180 s), and E = (239–240 s). The results showed that fipronil reduced the frequency and intensity of cardiac activity, exhibiting rapidly evolving effects, and promoting a disruption of homeostasis in bee hemodynamics. Through the obtained data, it was observed variation in spike amplitude, with a loss of cardiac strength and magnitude of the electrical impulse in the bee’s heart during exposure to fipronil.

Bibliographic Details

Main Authors:	PAZ, C. A. da, EIRÓ-QUIRINO, L., ARAÚJO, D. B. de, BARBOSA, G. B., CÂMARA, T. M., CAMPOS, A. L. C. de, SOUZA, L. V. de, SANTOS, R. N. O., HARTCOPFF, P. F. P., HAMOY, M. K. O., SANTOS, M. F. dos, FERREIRA, R. da C., DEIGA, Y. da S., RUSSO, A. P. L., PEREIRA, D. S., CONTRERA, F. A. L., MUTO, N. A., HAMOY, M.
Other Authors:	CLARISSA ARAÚJO DA PAZ, UNIVERSIDADE FEDERAL DO PARÁ; LUCIANA EIRÓ-QUIRINO, UNIVERSIDADE FEDERAL DO PARÁ; DANIELLA BASTOS DE ARAÚJO, UNIVERSIDADE FEDERAL DO PARÁ; GABRIELA BRITO BARBOSA, UNIVERSIDADE FEDERAL DO PARÁ; TAYS MATA CÂMARA, UNIVERSIDADE FEDERAL DO PARÁ; ANA LUIZA CORDEIRO DE CAMPOS, UNIVERSIDADE FEDERAL DO PARÁ; LUANA VASCONCELOS DE SOUZA, UNIVERSIDADE FEDERAL DO PARÁ; RAFAELA NEGRÃO OLÍVIA SANTOS, UNIVERSIDADE FEDERAL DO PARÁ; PRISCILLE FIDELIS PACHECO HARTCOPFF, UNIVERSIDADE FEDERAL DO PARÁ; MARIA KLARA OTAKE HAMOY, UNIVERSIDADE FEDERAL DO PARÁ; MURILO FARIAS DOS SANTOS, UNIVERSIDADE FEDERAL DO PARÁ; RAYLLAN DA CUNHA FERREIRA, UNIVERSIDADE FEDERAL DO PARÁ; YRIS DA SILVA DEIGA, UNIVERSIDADE FEDERAL DO PARÁ; ANTÔNIO PEDRO LISBOA RUSSO, UNIVERSIDADE FEDERAL DO PARÁ; DANIEL SANTIAGO PEREIRA, CPATU; FELIPE ANDRÉS LEÓN CONTRERA, UNIVERSIDADE FEDERAL DO PARÁ; NILTON AKIO MUTO, UNIVERSIDADE FEDERAL DO PARÁ; MOISÉS HAMOY, UNIVERSIDADE FEDERAL DO PARÁ.
Format:	Artigo de periódico biblioteca
Language:	eng
Published:	2024-08-09
Subjects:	Pesticides, Homeostasis, Honey bees, Toxicity,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1166411 https://doi.org/10.1007/s44338-024-00017-y
Tags:	Add Tag No Tags, Be the first to tag this record!