Data from: A fast method for the detection of <i>Salmonella</i> Typhimurium cells in egg biofilms on three different surfaces
<p><em>Salmonella</em> is a pathogenic microorganism linked to foodborne outbreaks associated with egg and egg products. This microorganism can resist sanitation of the egg processing equipment and form biofilms. The main challenge is detecting <em>Salmonella</em> cells in the early stages of biofilm formation to use effective interventions to control and remove the <em>Salmonella</em> biofilms. This work aimed to study the biofilm formation of <em>S.</em> Typhimurium in liquid whole egg (LWE) on three common food-contact surfaces, stainless steel, silicone, and nylon, during the first five hours of incubation at 37°C and compare traditional microbiological methods to innovative and fast detection techniques. The results showed that using general plate counts, <em>Salmonella</em> cells were detected after three h of incubation with less than 1 – log of growth, silicone was the material with most cells attached, followed by stainless steel. Long-read whole genome sequencing detected <em>Salmonella</em> on stainless steel, silicone, and nylon after only one h of incubation. The results of this study suggest that long-read sequencing could be very useful for detecting <em>Salmonella</em> at low concentrations in the processing environment.</p> <p><br></p> <p>This research used the resources provided by SCINet project and the AI Center of Excellence of the USDA Agricultural Research Service, ARS project number 0500-00093-001-00-D.</p>
| Main Authors: | , , , |
|---|---|
| Format: | Dataset biblioteca |
| Published: |
2024
|
| Subjects: | Agricultural, veterinary and food sciences, Biological sciences, Microbiology, Salmonella, food pathogens, egg, biofilms, long-read whole genome sequencing, bioinformatics, |
| Online Access: | https://figshare.com/articles/dataset/Data_from_A_fast_method_for_the_detection_of_i_Salmonella_i_Typhimurium_cells_in_egg_biofilms_on_three_different_surfaces/26866363 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|