Inheritance and genetic mapping of self-incompatibility in Coffea canephora Pierre
Cross-compatibility behaviour of doubled haploid (DH) and hybrid genotypes of Coffea canephora was established using both phenotipic bioassay and in situ seed-set examination. The availability of DHs provided the opportunity of working with genetically homogeneous pollen and female parents. The aniline blue fluorescence (ABF) method was applied to detect callose accumulation in pollen and pistil. Clear cross-compatibility/incompatibility situations were observed and confirmed by in situ seed-set analysis. Cross-compatibility analysis of hybrid combinations involving different. DHs corroborated the crossing behaviour observed at the DH level. Expression of the self-incompatibility system did not appear to be affected by the low vigour of the DH. The crossing-behaviour distribution observed within DHs derived from clone IF200 confirmed that self incompatibility in C. canephora is a gametophytic self-incompatibility system controlled by a single locus (S-locus). Reduced seed-set developments following in compatible crosses may indicate the occurrence of pseudo-incompatibility. Molecular marker linkage analysis showed that the S-locus is associated with an RFLP marker on linkage group 9. The availability of a linked DNA marker should facilitate the genetic analysis of self-incompatibility in relation to coffee breeding programmes.
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Montpellier (Francia)
1994
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Subjects: | COFFEA CANEPHORA, AUTOESTERILIDAD, GAMETOS, LOCI, RFLP, MARCADORES GENETICOS, |
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KOHA-OAI-BVE:887752020-02-03T21:56:27ZInheritance and genetic mapping of self-incompatibility in Coffea canephora PierreCoffee genetic resources and breeding: recent scientific papers 85180 Lashermes, P. 59669 Couturon, E. 96392 Moreau, N. 102645 Paillard, M. 87745 Louarn, J. 15687 ORSTOM, Montpellier (Francia). Laboratoire de Ressources Génétiques et Amélioration des Plantes Tropicales Montpellier (Francia)1994-1997Cross-compatibility behaviour of doubled haploid (DH) and hybrid genotypes of Coffea canephora was established using both phenotipic bioassay and in situ seed-set examination. The availability of DHs provided the opportunity of working with genetically homogeneous pollen and female parents. The aniline blue fluorescence (ABF) method was applied to detect callose accumulation in pollen and pistil. Clear cross-compatibility/incompatibility situations were observed and confirmed by in situ seed-set analysis. Cross-compatibility analysis of hybrid combinations involving different. DHs corroborated the crossing behaviour observed at the DH level. Expression of the self-incompatibility system did not appear to be affected by the low vigour of the DH. The crossing-behaviour distribution observed within DHs derived from clone IF200 confirmed that self incompatibility in C. canephora is a gametophytic self-incompatibility system controlled by a single locus (S-locus). Reduced seed-set developments following in compatible crosses may indicate the occurrence of pseudo-incompatibility. Molecular marker linkage analysis showed that the S-locus is associated with an RFLP marker on linkage group 9. The availability of a linked DNA marker should facilitate the genetic analysis of self-incompatibility in relation to coffee breeding programmes.Cross-compatibility behaviour of doubled haploid (DH) and hybrid genotypes of Coffea canephora was established using both phenotipic bioassay and in situ seed-set examination. The availability of DHs provided the opportunity of working with genetically homogeneous pollen and female parents. The aniline blue fluorescence (ABF) method was applied to detect callose accumulation in pollen and pistil. Clear cross-compatibility/incompatibility situations were observed and confirmed by in situ seed-set analysis. Cross-compatibility analysis of hybrid combinations involving different. DHs corroborated the crossing behaviour observed at the DH level. Expression of the self-incompatibility system did not appear to be affected by the low vigour of the DH. The crossing-behaviour distribution observed within DHs derived from clone IF200 confirmed that self incompatibility in C. canephora is a gametophytic self-incompatibility system controlled by a single locus (S-locus). Reduced seed-set developments following in compatible crosses may indicate the occurrence of pseudo-incompatibility. Molecular marker linkage analysis showed that the S-locus is associated with an RFLP marker on linkage group 9. The availability of a linked DNA marker should facilitate the genetic analysis of self-incompatibility in relation to coffee breeding programmes.COFFEA CANEPHORAAUTOESTERILIDADGAMETOSLOCIRFLPMARCADORES GENETICOS |
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COFFEA CANEPHORA AUTOESTERILIDAD GAMETOS LOCI RFLP MARCADORES GENETICOS COFFEA CANEPHORA AUTOESTERILIDAD GAMETOS LOCI RFLP MARCADORES GENETICOS |
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COFFEA CANEPHORA AUTOESTERILIDAD GAMETOS LOCI RFLP MARCADORES GENETICOS COFFEA CANEPHORA AUTOESTERILIDAD GAMETOS LOCI RFLP MARCADORES GENETICOS 85180 Lashermes, P. 59669 Couturon, E. 96392 Moreau, N. 102645 Paillard, M. 87745 Louarn, J. 15687 ORSTOM, Montpellier (Francia). Laboratoire de Ressources Génétiques et Amélioration des Plantes Tropicales Inheritance and genetic mapping of self-incompatibility in Coffea canephora Pierre |
description |
Cross-compatibility behaviour of doubled haploid (DH) and hybrid genotypes of Coffea canephora was established using both phenotipic bioassay and in situ seed-set examination. The availability of DHs provided the opportunity of working with genetically homogeneous pollen and female parents. The aniline blue fluorescence (ABF) method was applied to detect callose accumulation in pollen and pistil. Clear cross-compatibility/incompatibility situations were observed and confirmed by in situ seed-set analysis. Cross-compatibility analysis of hybrid combinations involving different. DHs corroborated the crossing behaviour observed at the DH level. Expression of the self-incompatibility system did not appear to be affected by the low vigour of the DH. The crossing-behaviour distribution observed within DHs derived from clone IF200 confirmed that self incompatibility in C. canephora is a gametophytic self-incompatibility system controlled by a single locus (S-locus). Reduced seed-set developments following in compatible crosses may indicate the occurrence of pseudo-incompatibility. Molecular marker linkage analysis showed that the S-locus is associated with an RFLP marker on linkage group 9. The availability of a linked DNA marker should facilitate the genetic analysis of self-incompatibility in relation to coffee breeding programmes. |
format |
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topic_facet |
COFFEA CANEPHORA AUTOESTERILIDAD GAMETOS LOCI RFLP MARCADORES GENETICOS |
author |
85180 Lashermes, P. 59669 Couturon, E. 96392 Moreau, N. 102645 Paillard, M. 87745 Louarn, J. 15687 ORSTOM, Montpellier (Francia). Laboratoire de Ressources Génétiques et Amélioration des Plantes Tropicales |
author_facet |
85180 Lashermes, P. 59669 Couturon, E. 96392 Moreau, N. 102645 Paillard, M. 87745 Louarn, J. 15687 ORSTOM, Montpellier (Francia). Laboratoire de Ressources Génétiques et Amélioration des Plantes Tropicales |
author_sort |
85180 Lashermes, P. |
title |
Inheritance and genetic mapping of self-incompatibility in Coffea canephora Pierre |
title_short |
Inheritance and genetic mapping of self-incompatibility in Coffea canephora Pierre |
title_full |
Inheritance and genetic mapping of self-incompatibility in Coffea canephora Pierre |
title_fullStr |
Inheritance and genetic mapping of self-incompatibility in Coffea canephora Pierre |
title_full_unstemmed |
Inheritance and genetic mapping of self-incompatibility in Coffea canephora Pierre |
title_sort |
inheritance and genetic mapping of self-incompatibility in coffea canephora pierre |
publisher |
Montpellier (Francia) |
publishDate |
1994 |
work_keys_str_mv |
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_version_ |
1756059865171623936 |