Inheritance and restriction fragment length polymorphism of chloroplast DNA in the genus Coffea L.

CpDNA variation among 52 tree samples belonging to 25 different taxa of Coffea and two species of Psilanthus was assessed by RFLP analysis on both the total chloroplast genome and the atpB-rbcL intergenic region. Twelve variable characters were distinguished allowing the identification of 12 different plastomes. The low sequence divergence observed might suggest that Coffea is a young genus. The results were in contradiction with the present classification into two genera. Additionally, cpDNA inheritance was studied in interspecific hybrids between C. arabica and C. canephora, and in an intraspecific progeny of C. canephora, using PCR-based markers. Both studies showed exclusively maternal inheritance of cpDNA.

Bibliographic Details

Main Authors:	85180 Lashermes, P., 59948 Cros, J., 58486 Combes, M.C., 125624 Trouslot, P., 43095 Anthony, F., 75866 Hamon, S., 56635 Charrier, A., 15687 ORSTOM, Montpellier (Francia). Laboratoire de Ressources Génétiques et Amélioration des Plantes Tropicales
Format:	biblioteca
Published:	Montpellier (Francia) 1994
Subjects:	COFFEA, CLOROPLASTO, HERENCIA GENETICA, RFLP, MAPAS GENETICOS, MARCADORES GENETICOS,
Tags:	Add Tag No Tags, Be the first to tag this record!