Changes in the resistance of detached coffee leaves by yeast extract filtrate and heat treatment
Application of filtrates of yeast extract to detached leaves of Coffea arabica cv. "Mundo Novo", 72 hr prior to the inoculation with urediniospores of Hemileia vastatrix, race XXIII, was shown to induce resistance to the pathogen without affecting the fungal germination and appressoria formation. Preinoculation heat treatment of detached leaves of cv. "Mundo Novo" was shown to enhance its susceptibility to the pathogen. On the other hand, when the same preinoculation heat treatment was applied to detached leaves of the resistant hybrid - "Híbrido de Timor" - the fungus developed through the mesophyll up to the palisade tissue, while in the untreated leaves the fungus was restricted to the first layer of mesophyll. Abnormalities in the fungal growth were observed in detached leaves of cv. "Mundo Novo", thirty five days after the inoculation when compared to the normal fungal development in attached leaves. In the former case, the intercellular mycelium was shown to grow outwards the leaf through the stomata, as an aerial mycelium. An abnormal appearance of protosori within the mesophyll, far from the substomatic cavity and near the palisada tissue, was also frequently observed
| Main Authors: | , , , |
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| Format: | biblioteca |
| Published: |
Dic
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| Subjects: | COFFEA ARABICA, HEMILEIA, ROYA, RESISTENCIA A LA ENFERMEDAD, SACCHAROMYCES CEREVISIAE, INOCULACION, ENFERMEDADES FUNGOSAS, MUNDO NOVO, HEMILEIA VASTATRIX, |
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