Proceedings
Caseous lymphadenitis, a worldwide disease of goats and sheep, is caused by Corynebacterium pseudotuberculosis (C. pseudotuberculosis). Several laboratory tests are available for the diagnosis of the infection, but none are completely satisfactory in terms of simplicity, accuracy, sensitivity and economy. An enzyme-linked immunosorbent assay (ELISA) is described for the detection of Corynebacterium pseudotuberculosis infection. Cell wall antigen was used to coat a solid phase and alkaline phosphatase was used to conjugate antigoat immunoglobulin. The technique was evaluated by: 1) monitoring the antibody level and abscess development in goats experimentally infected with C. pseudotuberculosis, 2) correlating abscess formation in naturally infected goats and 3) comparing the ELISA with the synergistic hemolysis inhibition (SHI) test. A significant ELISA antibody rise was evident two weeks post infection (pi) and peaked at five weeks pi, then declined slightly, but persisted. Abscesses were seen 8-10 weeks pi. All goats which had abscess were positive to ELISA. The ELISA is slightly more sensitive than SHI. The cell wall antigen is apparently specific, since the ELISA did not cross-react with sera from goats experimentally infected with Corynebacterium pyogenes
Main Authors: | , , , , |
---|---|
Format: | biblioteca |
Published: |
Scottsdale, AZ (EUA)
1982
|
Subjects: | CAPRINOS, SALUD ANIMAL, ENFERMEDADES DE LOS ANIMALES, CORYNEBACTERIUM, LINFADENITIS, DIAGNOSTICO, |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
KOHA-OAI-BVE:44126 |
---|---|
record_format |
koha |
spelling |
KOHA-OAI-BVE:441262020-01-23T20:21:08ZProceedings 118464 Shen, D.T. 80791 Jen, L.W. 73229 Gorham, J.R. 6757 Dairy Goat Journal Publishing Co., Scottsdale, AZ (EUA) 33022 3. International Conference on Goat Production and Disease Tucson, AZ (EUA) 10-15 Ene 1982 Scottsdale, AZ (EUA)1982Caseous lymphadenitis, a worldwide disease of goats and sheep, is caused by Corynebacterium pseudotuberculosis (C. pseudotuberculosis). Several laboratory tests are available for the diagnosis of the infection, but none are completely satisfactory in terms of simplicity, accuracy, sensitivity and economy. An enzyme-linked immunosorbent assay (ELISA) is described for the detection of Corynebacterium pseudotuberculosis infection. Cell wall antigen was used to coat a solid phase and alkaline phosphatase was used to conjugate antigoat immunoglobulin. The technique was evaluated by: 1) monitoring the antibody level and abscess development in goats experimentally infected with C. pseudotuberculosis, 2) correlating abscess formation in naturally infected goats and 3) comparing the ELISA with the synergistic hemolysis inhibition (SHI) test. A significant ELISA antibody rise was evident two weeks post infection (pi) and peaked at five weeks pi, then declined slightly, but persisted. Abscesses were seen 8-10 weeks pi. All goats which had abscess were positive to ELISA. The ELISA is slightly more sensitive than SHI. The cell wall antigen is apparently specific, since the ELISA did not cross-react with sera from goats experimentally infected with Corynebacterium pyogenesCaseous lymphadenitis, a worldwide disease of goats and sheep, is caused by Corynebacterium pseudotuberculosis (C. pseudotuberculosis). Several laboratory tests are available for the diagnosis of the infection, but none are completely satisfactory in terms of simplicity, accuracy, sensitivity and economy. An enzyme-linked immunosorbent assay (ELISA) is described for the detection of Corynebacterium pseudotuberculosis infection. Cell wall antigen was used to coat a solid phase and alkaline phosphatase was used to conjugate antigoat immunoglobulin. The technique was evaluated by: 1) monitoring the antibody level and abscess development in goats experimentally infected with C. pseudotuberculosis, 2) correlating abscess formation in naturally infected goats and 3) comparing the ELISA with the synergistic hemolysis inhibition (SHI) test. A significant ELISA antibody rise was evident two weeks post infection (pi) and peaked at five weeks pi, then declined slightly, but persisted. Abscesses were seen 8-10 weeks pi. All goats which had abscess were positive to ELISA. The ELISA is slightly more sensitive than SHI. The cell wall antigen is apparently specific, since the ELISA did not cross-react with sera from goats experimentally infected with Corynebacterium pyogenesCAPRINOSSALUD ANIMALENFERMEDADES DE LOS ANIMALESCORYNEBACTERIUMLINFADENITISDIAGNOSTICOURN:ISBN:0930848179 |
institution |
IICA |
collection |
Koha |
country |
Costa Rica |
countrycode |
CR |
component |
Bibliográfico |
access |
En linea Fisico |
databasecode |
cat-sibiica |
tag |
biblioteca |
region |
America Central |
libraryname |
Sistema de Bibliotecas IICA/CATIE |
topic |
CAPRINOS SALUD ANIMAL ENFERMEDADES DE LOS ANIMALES CORYNEBACTERIUM LINFADENITIS DIAGNOSTICO CAPRINOS SALUD ANIMAL ENFERMEDADES DE LOS ANIMALES CORYNEBACTERIUM LINFADENITIS DIAGNOSTICO |
spellingShingle |
CAPRINOS SALUD ANIMAL ENFERMEDADES DE LOS ANIMALES CORYNEBACTERIUM LINFADENITIS DIAGNOSTICO CAPRINOS SALUD ANIMAL ENFERMEDADES DE LOS ANIMALES CORYNEBACTERIUM LINFADENITIS DIAGNOSTICO 118464 Shen, D.T. 80791 Jen, L.W. 73229 Gorham, J.R. 6757 Dairy Goat Journal Publishing Co., Scottsdale, AZ (EUA) 33022 3. International Conference on Goat Production and Disease Tucson, AZ (EUA) 10-15 Ene 1982 Proceedings |
description |
Caseous lymphadenitis, a worldwide disease of goats and sheep, is caused by Corynebacterium pseudotuberculosis (C. pseudotuberculosis). Several laboratory tests are available for the diagnosis of the infection, but none are completely satisfactory in terms of simplicity, accuracy, sensitivity and economy. An enzyme-linked immunosorbent assay (ELISA) is described for the detection of Corynebacterium pseudotuberculosis infection. Cell wall antigen was used to coat a solid phase and alkaline phosphatase was used to conjugate antigoat immunoglobulin. The technique was evaluated by: 1) monitoring the antibody level and abscess development in goats experimentally infected with C. pseudotuberculosis, 2) correlating abscess formation in naturally infected goats and 3) comparing the ELISA with the synergistic hemolysis inhibition (SHI) test. A significant ELISA antibody rise was evident two weeks post infection (pi) and peaked at five weeks pi, then declined slightly, but persisted. Abscesses were seen 8-10 weeks pi. All goats which had abscess were positive to ELISA. The ELISA is slightly more sensitive than SHI. The cell wall antigen is apparently specific, since the ELISA did not cross-react with sera from goats experimentally infected with Corynebacterium pyogenes |
format |
|
topic_facet |
CAPRINOS SALUD ANIMAL ENFERMEDADES DE LOS ANIMALES CORYNEBACTERIUM LINFADENITIS DIAGNOSTICO |
author |
118464 Shen, D.T. 80791 Jen, L.W. 73229 Gorham, J.R. 6757 Dairy Goat Journal Publishing Co., Scottsdale, AZ (EUA) 33022 3. International Conference on Goat Production and Disease Tucson, AZ (EUA) 10-15 Ene 1982 |
author_facet |
118464 Shen, D.T. 80791 Jen, L.W. 73229 Gorham, J.R. 6757 Dairy Goat Journal Publishing Co., Scottsdale, AZ (EUA) 33022 3. International Conference on Goat Production and Disease Tucson, AZ (EUA) 10-15 Ene 1982 |
author_sort |
118464 Shen, D.T. |
title |
Proceedings |
title_short |
Proceedings |
title_full |
Proceedings |
title_fullStr |
Proceedings |
title_full_unstemmed |
Proceedings |
title_sort |
proceedings |
publisher |
Scottsdale, AZ (EUA) |
publishDate |
1982 |
work_keys_str_mv |
AT 118464shendt proceedings AT 80791jenlw proceedings AT 73229gorhamjr proceedings AT 6757dairygoatjournalpublishingcoscottsdaleazeua proceedings AT 330223internationalconferenceongoatproductionanddiseasetucsonazeua1015ene1982 proceedings |
_version_ |
1756053806076919808 |