Genetic diversity and spatial structure in a new distinct Theobroma cacao L. population in Bolivia
Cacao (Theobroma cacao L.) is an important economic crop in the Bolivian Amazon. Bolivian farmers both cultivate cacao, and extract fruits from wild stands in the Beni River region and in valleys of the Andes foothills. The germplasm group traditionally used is presently referred to as ''Cacao Nacional Boliviano'' (CNB). Using DNA fingerprinting technology based on microsatellite markers, we genotyped 164 Bolivian cacao accessions, including both cultivated and wild CNB accessions sampled from the Amazonian regions of La Paz and Beni, and compared their SSR profiles with 78 reference Forastero accessions from Amazonian cacao populations, including germplasm from the Ucayali region of Peru. Results of multivariate ordination and analysis of molecular variance show that CNB cacao has a unique genetic profile that is significantly different from the known cacao germplasm groups in South America. The results also show that cultivated CNB and wild CNB populations in the Beni River share a similar genetic profile, suggesting that the cultivated CNB is of indigenous origin in Bolivia. The level of genetic diversity, measured by allele richness and gene diversity in the Bolivian cacao, is moderately high, but was significantly lower than gene diversity in the other Amazonian cacao populations. Significant spatial genetic structure was detected in the wild CNB population, using analysis of autocorrelation (rc = 0.232; P\0.001) and Mantel tests (Rxy = 0.276; P\0.001). This finding is also highly valuable to support in situ conservation and sustainable use of CNB genetic diversity in Bolivia.
Main Authors: | 133374 Zhang, Dapeng, 91308 Martínez, Windson J. (autor/a), 81311 Johnnson, Elizabeth S., Somarriba, Eduardo 120490, 105027 Phillips Mora, Wilbert, 44848 Astorga Domian, Carlos, 94639 Mischke, Sue, 92883 Meinhardt, Lyndel W. |
---|---|
Format: | Texto biblioteca |
Language: | eng |
Published: |
Springer
2015
|
Online Access: | http://repositorio.bibliotecaorton.catie.ac.cr/bitstream/handle/11554/7814/Zhang_et_al_Genetic.pdf http://hdl.handle.net/11554/7814 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Genetic diversity and spatial structure in a new distinct Theobroma cacao L. population in Bolivia
by: Zhang, Dapeng, et al.
Published: (2015) -
Traditional varieties of cacao (Theobroma cacao) in Madagascar: their origin and dispersal revealed by SNP markers
by: Li, Yanmei, et al. -
Selecting a core set of nuclear SNP markers for molecular characterization of Arabica coffee (Coffea arabica L.) genetic resources
by: Zhang, Dapeng 133374, et al.
Published: (2021) -
Association mapping of seed and disease resistance traits in Theobroma cacao L.
by: Motilal, Lambert A., et al. -
Molecular charaterization of an earliest cacao (Theobroma cacao L.) collection from Upper Amazon using microsatellite DNA markers
by: Zhang, Dapeng, et al.