Non ‑ thermal plasmas afect plant growth and DNA methylation patterns in Glycine max

Non ‑ thermal plasmas afect plant growth and DNA methylation patterns in Glycine max

Non-thermal plasmas (NTP) are partially ionized gases that represent a promising technology for seed treatment to enhance seed health while promoting germination and vigor in a fast, cost-efective, and eco-friendly way. The seed treatment with NTP generates phenotypic variations in plants that could be related to changes in DNA methylation. This work analyses the efects of two diferent NTP: nitrogen for 3 min (PMN3) and oxygen for 2 min (PMO2) applied to soybean (Glycine max) seeds. Growth parameters of plants grown from treated and untreated seeds were evaluated at two growth stages: 6 and 20 days after sowing (DAS). MSAP (Methylation Sensitive Amplifed Polymorphism) markers were assayed to evaluate epigenetic changes induced by NTP treatments. Plants obtained from PMN3 and PMO2-treated seeds were phenotypically similar to each other: exhibited a superior growth at both stages. At 6 DAS root and shoot length and fresh weight surpassed the Control, while at 20 DAS root length and fresh and dry weight were higher than Control. PMN3 and PMO2 induced DNA methylation changes with respect to the Control plants, with higher diferentiation at 20 DAS than at 6 DAS. The epigenetic variability and the phenotypic variability correlated only at 20 DAS (R2=0.5). The observed phenotypic diferences among Control and NTP-treated plants could not be explained by overall changes in the methylation levels, but both demethylation and methylation changes at specifc loci appear to be operating in response to NTP treatments.

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Main Authors: Pérez Pizá, María Cecilia, Ibañez, V. N., Varela, A., Cejas, Ezequiel, Ferreyra, Matías, Chamorro Garcés, Juan C., Zilli, Carla G., Vallecorsa, Pablo, Balestrasse, Karina Beatriz
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Language:eng
Subjects:MSAP, EPIGENETICS, SOYBEAN, NON THERMAL PLASMA, ,
Online Access:http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=55012
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spelling KOHA-OAI-AGRO:550122023-09-25T15:03:28Zhttp://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=55012http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=AAGNon ‑ thermal plasmas afect plant growth and DNA methylation patterns in Glycine maxPérez Pizá, María CeciliaIbañez, V. N.Varela, A.Cejas, EzequielFerreyra, MatíasChamorro Garcés, Juan C.Zilli, Carla G.Vallecorsa, PabloBalestrasse, Karina Beatriztextengapplication/pdfNon-thermal plasmas (NTP) are partially ionized gases that represent a promising technology for seed treatment to enhance seed health while promoting germination and vigor in a fast, cost-efective, and eco-friendly way. The seed treatment with NTP generates phenotypic variations in plants that could be related to changes in DNA methylation. This work analyses the efects of two diferent NTP: nitrogen for 3 min (PMN3) and oxygen for 2 min (PMO2) applied to soybean (Glycine max) seeds. Growth parameters of plants grown from treated and untreated seeds were evaluated at two growth stages: 6 and 20 days after sowing (DAS). MSAP (Methylation Sensitive Amplifed Polymorphism) markers were assayed to evaluate epigenetic changes induced by NTP treatments. Plants obtained from PMN3 and PMO2-treated seeds were phenotypically similar to each other: exhibited a superior growth at both stages. At 6 DAS root and shoot length and fresh weight surpassed the Control, while at 20 DAS root length and fresh and dry weight were higher than Control. PMN3 and PMO2 induced DNA methylation changes with respect to the Control plants, with higher diferentiation at 20 DAS than at 6 DAS. The epigenetic variability and the phenotypic variability correlated only at 20 DAS (R2=0.5). The observed phenotypic diferences among Control and NTP-treated plants could not be explained by overall changes in the methylation levels, but both demethylation and methylation changes at specifc loci appear to be operating in response to NTP treatments.Non-thermal plasmas (NTP) are partially ionized gases that represent a promising technology for seed treatment to enhance seed health while promoting germination and vigor in a fast, cost-efective, and eco-friendly way. The seed treatment with NTP generates phenotypic variations in plants that could be related to changes in DNA methylation. This work analyses the efects of two diferent NTP: nitrogen for 3 min (PMN3) and oxygen for 2 min (PMO2) applied to soybean (Glycine max) seeds. Growth parameters of plants grown from treated and untreated seeds were evaluated at two growth stages: 6 and 20 days after sowing (DAS). MSAP (Methylation Sensitive Amplifed Polymorphism) markers were assayed to evaluate epigenetic changes induced by NTP treatments. Plants obtained from PMN3 and PMO2-treated seeds were phenotypically similar to each other: exhibited a superior growth at both stages. At 6 DAS root and shoot length and fresh weight surpassed the Control, while at 20 DAS root length and fresh and dry weight were higher than Control. PMN3 and PMO2 induced DNA methylation changes with respect to the Control plants, with higher diferentiation at 20 DAS than at 6 DAS. The epigenetic variability and the phenotypic variability correlated only at 20 DAS (R2=0.5). The observed phenotypic diferences among Control and NTP-treated plants could not be explained by overall changes in the methylation levels, but both demethylation and methylation changes at specifc loci appear to be operating in response to NTP treatments.MSAPEPIGENETICSSOYBEANNON THERMAL PLASMAJournal of plant growth regulation
institution UBA FA
collection Koha
country Argentina
countrycode AR
component Bibliográfico
access En linea
En linea
databasecode cat-ceiba
tag biblioteca
region America del Sur
libraryname Biblioteca Central FAUBA
language eng
topic MSAP
EPIGENETICS
SOYBEAN
NON THERMAL PLASMA

MSAP
EPIGENETICS
SOYBEAN
NON THERMAL PLASMA
spellingShingle MSAP
EPIGENETICS
SOYBEAN
NON THERMAL PLASMA

MSAP
EPIGENETICS
SOYBEAN
NON THERMAL PLASMA
Pérez Pizá, María Cecilia
Ibañez, V. N.
Varela, A.
Cejas, Ezequiel
Ferreyra, Matías
Chamorro Garcés, Juan C.
Zilli, Carla G.
Vallecorsa, Pablo
Balestrasse, Karina Beatriz
Non ‑ thermal plasmas afect plant growth and DNA methylation patterns in Glycine max
description Non-thermal plasmas (NTP) are partially ionized gases that represent a promising technology for seed treatment to enhance seed health while promoting germination and vigor in a fast, cost-efective, and eco-friendly way. The seed treatment with NTP generates phenotypic variations in plants that could be related to changes in DNA methylation. This work analyses the efects of two diferent NTP: nitrogen for 3 min (PMN3) and oxygen for 2 min (PMO2) applied to soybean (Glycine max) seeds. Growth parameters of plants grown from treated and untreated seeds were evaluated at two growth stages: 6 and 20 days after sowing (DAS). MSAP (Methylation Sensitive Amplifed Polymorphism) markers were assayed to evaluate epigenetic changes induced by NTP treatments. Plants obtained from PMN3 and PMO2-treated seeds were phenotypically similar to each other: exhibited a superior growth at both stages. At 6 DAS root and shoot length and fresh weight surpassed the Control, while at 20 DAS root length and fresh and dry weight were higher than Control. PMN3 and PMO2 induced DNA methylation changes with respect to the Control plants, with higher diferentiation at 20 DAS than at 6 DAS. The epigenetic variability and the phenotypic variability correlated only at 20 DAS (R2=0.5). The observed phenotypic diferences among Control and NTP-treated plants could not be explained by overall changes in the methylation levels, but both demethylation and methylation changes at specifc loci appear to be operating in response to NTP treatments.
format Texto
topic_facet
MSAP
EPIGENETICS
SOYBEAN
NON THERMAL PLASMA
author Pérez Pizá, María Cecilia
Ibañez, V. N.
Varela, A.
Cejas, Ezequiel
Ferreyra, Matías
Chamorro Garcés, Juan C.
Zilli, Carla G.
Vallecorsa, Pablo
Balestrasse, Karina Beatriz
author_facet Pérez Pizá, María Cecilia
Ibañez, V. N.
Varela, A.
Cejas, Ezequiel
Ferreyra, Matías
Chamorro Garcés, Juan C.
Zilli, Carla G.
Vallecorsa, Pablo
Balestrasse, Karina Beatriz
author_sort Pérez Pizá, María Cecilia
title Non ‑ thermal plasmas afect plant growth and DNA methylation patterns in Glycine max
title_short Non ‑ thermal plasmas afect plant growth and DNA methylation patterns in Glycine max
title_full Non ‑ thermal plasmas afect plant growth and DNA methylation patterns in Glycine max
title_fullStr Non ‑ thermal plasmas afect plant growth and DNA methylation patterns in Glycine max
title_full_unstemmed Non ‑ thermal plasmas afect plant growth and DNA methylation patterns in Glycine max
title_sort non ‑ thermal plasmas afect plant growth and dna methylation patterns in glycine max
url http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=55012
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http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
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